A5025310673- DNA Repair Mechanisms
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Autophagy in Disease and Therapy
- Chromosomal and Genetic Variations
- Streptococcal Infections and Treatments
- Genomics and Chromatin Dynamics
- Inflammasome and immune disorders
National Institutes of Health
2023
Case Western Reserve University
2023
The Y-family DNA polymerases – Pol ι, η, κ and Rev1 are most well-known for their roles in the damage tolerance pathway of translesion synthesis (TLS). They function to overcome replication barriers by bypassing lesions that cannot be normally replicated, allowing forks continue without stalling. In this work, we demonstrate a novel interaction between each polymerase nucleotide excision repair (NER) proteins, RAD23A RAD23B. We initially focus on through series biochemical, cell-based,...
DNA polymerase eta (Pol η) is a Y-family translesion responsible for synthesizing new across UV-damaged templates. It recruited to replication forks following mono-ubiquitination of the PCNA clamp. This interaction mediated by PCNA-interacting protein (PIP) motifs within Pol η, as well its C-terminal ubiquitin-binding zinc finger (UBZ) domain. Previous work has suggested that η itself mono-ubiquitinated at four lysine residues, which dependent on prior UBZ Here, we show can be modified same...