A5054600311- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Advanced biosensing and bioanalysis techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- CRISPR and Genetic Engineering
- Neuroscience and Neuropharmacology Research
- Biosensors and Analytical Detection
- Animal Genetics and Reproduction
- Genetics, Bioinformatics, and Biomedical Research
- Plant Virus Research Studies
- Advanced Biosensing Techniques and Applications
- Bacteriophages and microbial interactions
- Ion channel regulation and function
Purdue University West Lafayette
2023
State Street (United States)
2023
University of California, Riverside
2022
The University of Texas Health Science Center at San Antonio
1993
A general method to generate biosensors for user-defined molecules could provide detection tools a wide range of biological applications. Here, we describe an approach the rapid engineering using PYR1 (Pyrabactin Resistance 1), plant abscisic acid (ABA) receptor with malleable ligand-binding pocket and requirement ligand-induced heterodimerization, which facilitates construction sense-response functions. We applied this platform evolve 21 sensors nanomolar micromolar sensitivities small...
Excitotoxic neuronal injury is known to be associated with increases in cytosolic calcium ion concentrations. However, it not if perturbations other intracellular ions are also glutamate (GLU)-induced death. Accordingly, hydrogen concentrations were measured cultured hippocampal neurons the fluorescent dye BCECF during and after toxic exposures. Five minute GLU applications produced an initial acidification. During hour removal, pH (pHi) recovered steadily, resulting a rebound...