A5089854882- Light effects on plants
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Genomics and Chromatin Dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant Gene Expression Analysis
- Plant-Microbe Interactions and Immunity
- RNA Research and Splicing
- Plant Reproductive Biology
- Epigenetics and DNA Methylation
- Plant Virus Research Studies
- Circadian rhythm and melatonin
- Plant and Biological Electrophysiology Studies
- Plant Pathogens and Fungal Diseases
- Chromosomal and Genetic Variations
- Cassava research and cyanide
- Plant Pathogenic Bacteria Studies
- Plant Pathogens and Resistance
- Greenhouse Technology and Climate Control
- Gene Regulatory Network Analysis
- Fungal and yeast genetics research
- Advanced Fluorescence Microscopy Techniques
- Genomics, phytochemicals, and oxidative stress
Donald Danforth Plant Science Center
2016-2025
Washington University in St. Louis
2021
University of California, San Diego
2010-2015
University of California System
2012
La Jolla Alcohol Research
2012
University of California, San Francisco
2002-2008
Scripps Research Institute
2007
University of California, Los Angeles
2002-2005
Precise timing of CONSTANS ( CO ) gene expression is necessary for day-length discrimination photoperiodic flowering. The FLAVIN-BINDING, KELCH REPEAT, F-BOX 1 (FKF1), and GIGANTEA (GI) proteins regulate transcription in Arabidopsis . We demonstrate that FKF1 GI form a complex blue-light–dependent manner. this interaction regulates the daytime expression. function dependent on GI, which interacts with repressor, CYCLING DOF FACTOR (CDF1), controls CDF1 stability. FKF1, associate chromatin....
Genome biology approaches have made enormous contributions to our understanding of biological rhythms, particularly in identifying outputs the clock, including RNAs, proteins, and metabolites, whose abundance oscillates throughout day. These methods hold significant promise for future discovery, when combined with computational modeling. However, genome-scale experiments are costly laborious, yielding “big data” that conceptually statistically difficult analyze. There is no obvious consensus...
CRISPR/Cas9 has become a powerful genome-editing tool for introducing genetic changes into crop species. In order to develop capacity technology in the tropical staple cassava (Manihot esculenta), Phytoene desaturase (MePDS) gene was targeted two cultivars using constructs carrying gRNAs targeting sequences within MePDS exon 13. After Agrobacterium-mediated delivery of reagents cells, both induced visible albino phenotypes cotyledon-stage somatic embryos regenerating on selection medium and...
Many species possess an endogenous circadian clock to synchronize internal physiology with oscillating external environment. In plants, the coordinates growth, metabolism and development over daily seasonal time scales. proteins in network form complexes that temporally regulate myriad processes, including signal transduction, transcription, protein degradation post-translational modification. Arabidopsis thaliana, a tripartite complex composed of EARLY FLOWERING 4 (ELF4), 3 (ELF3), LUX...
Abstract Wild and weedy relatives of domesticated crops harbor genetic variants that can advance agricultural biotechnology. Here we provide a genome resource for the wild plant green millet ( Setaria viridis ), model species studies C 4 grasses, use to probe domestication genes in close crop relative foxtail italica ). We produced platinum-quality assembly S. de novo assemblies 598 accessions exploited these identify loci underlying three traits: response climate, ‘loss shattering’ trait...
X inactivation involves the stable silencing of one two chromosomes in XX female mammals. Initiation this process occurs during early development and Xist (X-inactive-specific transcript) RNA coating recruitment Polycomb repressive complex (PRC) 2 PRC1 proteins. This results an inactive state that is initially labile but further locked by epigenetic marks such as DNA methylation, histone hypoacetylation, MACROH2A deposition. Here, we report E3 ubiquitin ligase consisting SPOP CULLIN3 able to...
Biological timekeeping is essential for proper growth and development. Organisms such as the model plant Arabidopsis use circadian clock to coordinate biological processes with environment so that changes in conditions are anticipated favorably phased. Despite identification of numerous genes, knowledge their molecular connectivity influence on output programs remains limited. We recently showed LUX encodes a sequence-specific DNA-binding protein directly regulates expression morning gene...
Premise of the Study Image‐based phenomics is a powerful approach to capture and quantify plant diversity. However, commercial platforms that make consistent image acquisition easy are often cost‐prohibitive. To high‐throughput phenotyping methods more accessible, low‐cost microcomputers cameras can be used acquire data. Methods Results We Raspberry Pi computers manage Detailed here three different applications Pi–controlled imaging for seed shoot imaging. Images obtained from each platform...
Plants react to seasonal change in day length through altering physiology and development. Factors that function harmonize growth with photoperiod are poorly understood. Here we characterize a new protein associates both circadian clock photoreceptor components, named PHOTOPERIODIC CONTROL OF HYPOCOTYL1 (PCH1). pch1 seedlings have overly elongated hypocotyls specifically under short days while constitutive expression of PCH1 shortens independent length. peaks at dusk, binds phytochrome B...
Abstract The OLIGOPEPTIDE TRANSPORTER 3 (OPT3) has recently been identified as a component of the systemic network mediating iron (Fe) deficiency responses in Arabidopsis. Reduced expression OPT3 induces an over accumulation Fe roots and leaves, due part by elevated IRON‐REGULATED 1. Here we show however, that opt3 leaves display transcriptional program consistent with overload, suggesting excess is properly sensed OPT3‐mediated shoot‐to‐root signaling critical to prevent overload. We also...
A general method to generate biosensors for user-defined molecules could provide detection tools a wide range of biological applications. Here, we describe an approach the rapid engineering using PYR1 (Pyrabactin Resistance 1), plant abscisic acid (ABA) receptor with malleable ligand-binding pocket and requirement ligand-induced heterodimerization, which facilitates construction sense-response functions. We applied this platform evolve 21 sensors nanomolar micromolar sensitivities small...
The plant hormone abscisic acid (ABA) controls growth and development regulates water status through an established signaling pathway. In the presence of ABA, pyrabactin resistance/regulatory component ABA receptor proteins inhibit type 2C protein phosphatases (PP2Cs). This, in turn, enables activation Sucrose Nonfermenting1-Related Protein Kinases2 (SnRK2). Open Stomata1 (OST1)/SnRK2.6/SRK2E is a major SnRK2-type kinase responsible for mediating responses. Arabidopsis (Arabidopsis thaliana)...
Significance In Arabidopsis , phytochrome B (phyB) perceives light and temperature signals to regulate various fundamental morphogenic processes in plants through its interconversion between active Pfr inactive Pr states. On photoconversion from Pfr, phyB forms subnuclear foci called photobodies, whose compositions molecular functions are unclear. We show here that the phyB-interacting protein PCH1 is a structural component of photobodies protects thermal reversion back Pr, thereby helping...
Abstract C 4 plants frequently experience high light and temperature conditions in the field, which reduce growth yield. However, mechanisms underlying these stress responses have been under-explored, especially coordination between mesophyll (M) bundle sheath (BS) cells. We investigated how model plant Setaria viridis responded to a four-hour or treatment at photosynthetic, transcriptomic, ultrastructural levels. Although we observed comparable reduction of photosynthetic efficiency treated...
Abstract Plants sense abscisic acid (ABA) using chemical-induced dimerization (CID) modules, including the receptor PYR1 and HAB1, a phosphatase inhibited by ligand-activated PYR1. This system is unique because of relative ease with which ligand recognition can be reprogrammed. To expand system, we designed an orthogonal ‘*’ module, harbors dimer interface salt bridge; X-ray crystallographic, biochemical in vivo analyses confirm its orthogonality. We used this module to create PYR1* MANDI...