A5070431126- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Bioenergy crop production and management
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Crop Yield and Soil Fertility
- Legume Nitrogen Fixing Symbiosis
- Ubiquitin and proteasome pathways
- Algal biology and biofuel production
- Plant Stress Responses and Tolerance
- Protein Degradation and Inhibitors
- Erythrocyte Function and Pathophysiology
- Plant Reproductive Biology
- Light effects on plants
- Calcium Carbonate Crystallization and Inhibition
- Blood properties and coagulation
- Marine Biology and Environmental Chemistry
- Fungal and yeast genetics research
- Soybean genetics and cultivation
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- RNA Research and Splicing
- Polysaccharides and Plant Cell Walls
- Lipid metabolism and biosynthesis
- Soil Carbon and Nitrogen Dynamics
University of Nebraska–Lincoln
2016-2025
Donald Danforth Plant Science Center
2015-2019
Norwich Research Park
1997-2014
John Innes Centre
1997-2014
Many species possess an endogenous circadian clock to synchronize internal physiology with oscillating external environment. In plants, the coordinates growth, metabolism and development over daily seasonal time scales. proteins in network form complexes that temporally regulate myriad processes, including signal transduction, transcription, protein degradation post-translational modification. Arabidopsis thaliana, a tripartite complex composed of EARLY FLOWERING 4 (ELF4), 3 (ELF3), LUX...
Plants react to seasonal change in day length through altering physiology and development. Factors that function harmonize growth with photoperiod are poorly understood. Here we characterize a new protein associates both circadian clock photoreceptor components, named PHOTOPERIODIC CONTROL OF HYPOCOTYL1 (PCH1). pch1 seedlings have overly elongated hypocotyls specifically under short days while constitutive expression of PCH1 shortens independent length. peaks at dusk, binds phytochrome B...
Abstract Honey has been used as a nutrient, an ointment, and medicine worldwide for many centuries. Modern research demonstrated that honey medicinal properties, reflected in its anti‐microbial, anti‐oxidant, anti‐inflammatory bioactivities. is composed of sugars, water myriad minor components, including minerals, vitamins, proteins polyphenols. Here, we report new bioactive component‒vesicle‐like nanoparticles‒in (H‐VLNs). These H‐VLNs are membrane‐bound nano‐scale particles contain lipids,...
Aberrant activation of the nucleotide-binding domain and leucine-rich repeat related (NLR) family, pyrin containing 3 (NLRP3) inflammasome drives development many complex inflammatory diseases, such as obesity, Alzheimer's disease, atherosclerosis. However, no medications specifically targeting NLRP3 have become clinically available. Therefore, we aim to identify new inhibitors in this study. Methods: Vesicle-like nanoparticles (VLNs) were extracted from garlic chives other Allium vegetables...
The soybean root system is complex. In addition to being composed of various cell types, the includes primary root, lateral roots, and nodule, an organ in which mutualistic symbiosis with N-fixing rhizobia occurs. A mature nodule characterized by a central infection zone where atmospheric nitrogen fixed assimilated symbiont, resulting from close cooperation between plant bacteria. To date, transcriptome individual cells isolated developing nodules has been established, but transcriptomic...
The role of long non-coding RNAs (lncRNAs) in regulating endothelial function through the DNA damage response (DDR) remains poorly understood. In this study, we demonstrate that lncRNA maternally expressed gene 3 (Meg3) interacts with RNA binding protein polypyrimidine tract (PTBP3) to regulate expression and p53 signaling ─ a major coordinator apoptosis cell proliferation triggered by DDR. Meg3 is induced cells (ECs) upon activation. silencing induces damage, activates signaling, increases...
A gene encoding an antifreeze protein (AFP) was isolated from carrot ( Daucus carota ) using sequence information derived the purified protein. The AFP is highly similar to polygalacturonase inhibitor (PGIP) family of apoplastic plant leucine‐rich repeat (LRR) proteins. Expression rapidly induced by low temperatures. Furthermore, expression in transgenic Arabidopsis thaliana plants leads accumulation activity. Our findings suggest that a new type has recently evolved PGIPs.
Solidified adhesive (cement) of three balanid barnacles (Crustacea: Cirripedia) was dissolved using different concentrations sodium dodecyl sulphate containing a reducing agent. The proteins were separated SDS-PAGE and blotted onto polyvinylidene difluoride membrane for sequencing. Commonly occurring bands in the cement each species identified. One particular protein 39 kD, found Balanus perforatus , has following N-terminal sequence: TYFPVLSYG?SSSLAPVI, where the? is most likely cysteine....
Rhizobium leguminosarum bv. viciae normally gains access to pea host cells through tubular cell wall ingrowths termed infection threads. Matrix glycoprotein (MGP), a major component of the thread lumen, is also secreted from tips uninoculated roots and can be released into solution under reducing conditions. Monoclonal antibody MAC265, which recognizes MGP carbohydrate epitope, was used for immunoaffinity purification roots. Following treatment with chymotrypsin, peptide fragment obtained...
The alpha phenol-soluble modulins (αPSMs) are among the most potent toxins produced by Staphylococcus aureus . Their biological role during infection has been studied in detail; however, way they bacterial cell is not well understood. In this work, we identify a small RNA molecule called Teg41 that plays an important αPSM production S. positively influences production. importance of highlighted fact strain containing deletion 3′ end produces significantly less αPSMs and attenuated for...
Indol-3-yl acetic acid (IAA, auxin) is a plant hormone whose degradation key determinant of growth and development. The first evidence for skatolyl hydroperoxide formation during the peroxidase-catalysed IAA has been obtained by electrospray MS. Skatolyl degrades predominantly non-enzymically to oxindol-3-yl carbinol but in part enzymically into indol-3-yl methanol via peroxidase cycle which acts as an electron donor. degradable catalase. Horseradish isoenzyme C (HRP-C) anionic tobacco (TOP)...
During the transition from seed to seedling, emerging embryos strategically balance available resources between building up defenses against environmental threats and initiating developmental program that promotes switch autotrophy. We present evidence of a critical role for phenylalanine (Phe) biosynthetic activity AROGENATE DEHYDRATASE3 (ADT3) in coordinating reactive oxygen species (ROS) homeostasis cotyledon development etiolated Arabidopsis (Arabidopsis thaliana) seedlings. show ADT3 is...
Hibernating mammals such as the thirteen-lined ground squirrel (Ictidomys tridecemlineatus) experience significant reductions in oxidative metabolism and body temperature when entering a state known torpor. Animals or exiting torpor do not permanent loss of brain function other injuries, processes that enable neuroprotection are well understood. To gain insight into changes protein occur dramatically different physiological states hibernation, we performed quantitative phosphoproteomics...
Lignin is a universal waste product of the agricultural industry and currently seen as potential feedstock for more sustainable manufacturing. While it second most abundant biopolymer in world, burned very recalcitrant material. Many recent studies, however, have demonstrated viability biocatalysis to improve value this convert into useful chemicals, such polyhydroxybutyrate, clean fuels like hydrogen n-butanol. Rhodopseudomonas palustris gram-negative bacterium which demonstrates plethora...
Plant responses to the environment are shaped by external stimuli and internal signaling pathways. In both model plant Arabidopsis thaliana (Arabidopsis) crop species, circadian clock factors critical for growth, flowering, rhythms. Outside of Arabidopsis, however, little is known about molecular function gene products. Therefore, we sought compare Brachypodium distachyon (Brachypodium) Setaria viridis (Setaria) orthologs EARLY FLOWERING 3, a key in Arabidopsis. To identify cycling genes...
Normal mammalian brain aging is characterized by the selective loss of discrete populations dendritic spines and synapses, particularly affecting neuroanatomical regions such as hippocampus. Although previous investigations have quantified this morphologically, molecular pathways orchestrating preferential synaptic vulnerability remain to be elucidated. Using quantitative proteomics healthy rhesus macaque human patient regional tissues, we comprehensively profiled temporal expression...
Despite ongoing efforts to employ structure-based methods discover targeted protein degraders (TPD), the prevailing strategy remains synthesizing a focused set of heterobifunctional compounds and screening them for target degradation.
The study aimed to analyze pH and heat treatment's effect in modulating the release of peptides with antioxidant activity after simulated gastrointestinal (GI) digestion Egg white powder (EWP). EWP samples neutral (EWPN) alkaline (EWPA) were heat-treated at 20, 60, 90 °C analyzed for protein aggregation, solubility, GI digestibility. Heat treatment decreased solubility induced which was higher EWPN as compared EWPA. unfolding EWPA proteins 60 exhibited a digestibility via Oxygen Radical...
Grapevine is a large source of healthy polyphenols for human diet, and red table‐grapes wines are the main stilbenes. These compounds important both in plant defence system health. In present study, Vitis vinifera cv. Barbera cell cultures were treated with 50 μg/mL chitosan proteomic analyses on soluble membrane subcellular fractions performed against suitable controls. Three stilbene synthase protein spots, four spots microsomal fraction ATPase subunits identified, accumulation which was...
Lipid droplets (LDs) are composed of neutral lipids enclosed in a phospholipid monolayer, which harbors membrane-associated proteins that regulate LD functions. Despite the crucial role LDs lipid metabolism, remodeling protein composition disease contexts, such as steatosis, remains poorly understood. We hypothesized chronic ethanol consumption, subsequent abstinence from ethanol, or fasting differentially affects membrane proteome content and these changes influence how interact with other...
Fusarium head blight (FHB) caused by graminearum is one of the most devastating diseases wheat and barley worldwide. Effectors suppress host immunity promote disease development. The genome F. contains hundreds effectors with unknown function. Therefore, investigations functions these will facilitate developing novel strategies to enhance resistance FHB. We characterized a effector, FgNls1, containing signal peptide multiple eukaryotic nuclear localization signals. A fusion protein green...
Spaceflight presents a unique environment with complex stressors, including microgravity and radiation, that can influence plant physiology at molecular levels. Combining transcriptomics proteomics approaches, this research gives insights into the coordination of transcriptome proteome in Arabidopsis’ physiological responses to environmental stress. Arabidopsis seedlings were germinated grown (µ g ) aboard International Space Station (ISS) NASA Biological Research Canisters – Light Emitting...