A5110296830- Cardiac electrophysiology and arrhythmias
- Ion channel regulation and function
- Cancer Genomics and Diagnostics
- Lung Cancer Treatments and Mutations
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Pancreatic and Hepatic Oncology Research
- Cardiovascular Effects of Exercise
- Receptor Mechanisms and Signaling
- Anesthesia and Sedative Agents
- Alzheimer's disease research and treatments
- Protein Degradation and Inhibitors
- Ubiquitin and proteasome pathways
- PI3K/AKT/mTOR signaling in cancer
- Renal cell carcinoma treatment
- CAR-T cell therapy research
- Circular RNAs in diseases
- Lung Cancer Research Studies
- Nausea and vomiting management
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Cancer-related Molecular Pathways
- Intensive Care Unit Cognitive Disorders
- RNA modifications and cancer
- Cancer Mechanisms and Therapy
- Computational Drug Discovery Methods
Mayo Clinic
2006-2025
First Affiliated Hospital of Hunan University of Traditional Chinese Medicine
2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2024-2025
China Pharmaceutical University
2024
Columbia University
1997-2024
Cancer Genetics (United States)
2001-2024
Mayo Clinic in Arizona
2013-2024
WinnMed
2018-2024
Jilin Province Tumor Hospital
2024
City of Hope
2024
PTEN phosphatase acts as a tumor suppressor by negatively regulating the phosphoinositide 3-kinase (PI3K) signaling pathway. It is unclear which downstream components of this pathway are necessary for oncogenic transformation. In report we show that transformed cells +/− mice have elevated levels phosphorylated Akt and activated p70/S6 kinase associated with an increase in proliferation. Pharmacological inactivation mTOR/RAFT/FRAP reduced neoplastic proliferation, size, activity, but did not...
Abstract In the current study, we examined a panel of serially passaged glioblastoma xenografts, in context an intracranial tumor therapy response model, to identify associations between molecular characteristics and sensitivity epidermal growth factor receptor (EGFR) kinase inhibitor erlotinib. From initial evaluation 11 distinct two erlotinib-sensitive tumors were identified, each having amplified EGFR expressing wild-type PTEN. One these expressed truncated EGFRvIII, whereas other...
Voltage-dependent Ca 2+ channel function (Ca v 1.2, L-type channel) is required for cardiac excitation-contraction (E-C) coupling. 1.2 plays a key role in modulating response to classic signaling pathways, such as the renin-angiotensin system and sympathetic nervous system. Regulation of contraction by neurotransmitters hormones often correlated with current through actions cAMP cGMP. Cardiac cGMP, which activates protein kinase G (PKG), regulated nitric oxide (NO), natriuretic peptides....
Increased sodium influx via incomplete inactivation of the major cardiac channel Na(V)1.5 is correlated with an increased incidence atrial fibrillation (AF) in humans. Here, we sought to determine whether entry sufficient cause structural and electrophysiological perturbations that are required initiate sustain AF. We used mice expressing a human variant mutation anesthetic-binding site (F1759A-Na(V)1.5) demonstrated Na+ drive alterations, including ventricular enlargement, myofibril...
Abstract The essential trace element zinc (Zn) is widely required in cellular functions, and abnormal Zn homeostasis causes a variety of health problems including immunodeficiency sensory dysfunctions. Previous studies had shown that availability was also important for tumor growth progression. aim the present study to investigate potential mechanisms N,N,N,N‐Tetrakis(2‐pyridylmethyl)‐ethylenediamine (TPEN) (a membrane permeable chelator) induced pancreatic cancer cell death. text...
Fight-or-flight responses involve β-adrenergic-induced increases in heart rate and contractile force. In the present study, we uncover primary mechanism underlying heart's innate reserve. We show that four protein kinase A (PKA)-phosphorylated residues Rad, a calcium channel inhibitor, are crucial for controlling basal current essential β-adrenergic augmentation of influx cardiomyocytes. Even with intact PKA signaling to other proteins modulating handling, preventing adrenergic activation...
This study was undertaken to characterize preclinical cytotoxic interactions for human malignancies between the multikinase inhibitor sorafenib (BAY 43-9006) and proteasome inhibitors bortezomib or MG132. Multiple tumor cell lines of varying histiotypes, including A549 (lung adenocarcinoma), 786-O (renal carcinoma), HeLa (cervical MDA-MB-231 (breast), K562 (chronic myelogenous leukemia), Jurkat (acute T-cell MEC-2 (B-chronic lymphocytic U251 D37 (glioma), as well cells derived from primary...
The cardiac voltage-gated Ca2+ channel, Cav1.2, mediates excitation-contraction coupling in the heart. molecular composition of channel includes pore-forming α1 subunit and auxiliary α2/δ-1 β subunits. γ subunits, which there are 8 isoforms, consist 4 transmembrane domains with intracellular N- C-terminal ends. γ1 was initially detected skeletal muscle Cav1.1 complex, modulating current amplitude activation inactivation properties. also shifts steady-state to more negative membrane...
Skeletal myogenesis is a highly coordinated process that involves cell proliferation, differentiation and fusion controlled by complex gene regulatory network. The microRNA cluster miR-17-92 has been shown to be related this process; however, the exact role of each member remains unclear. Here, we show miR-17 miR-20a could effectively promote both C2C12 myoblasts primary bovine satellite cells. In contrast, miR-18a might play negative in differentiation, while miR-19 miR-92a had little...
Rationale: Changing activity of cardiac Ca V 1.2 channels under basal conditions, during sympathetic activation, and in heart failure is a major determinant physiology pathophysiology. Although are prominently upregulated via activation PKA (protein kinase A), essential molecular details remained stubbornly enigmatic. Objective: The primary goal this study was to determine how various factors converging at the I-II loop interact regulate channel β-adrenergic stimulation, failure. Methods...
Circular RNAs (circRNAs) are a novel class of widespread noncoding that regulate gene expression in mammals. Recent studies demonstrate functional peptides can be encoded by short open reading frames RNAs, including circRNAs. However, the role circRNAs various physiological and pathological states, such as cancer, is not well understood. In this study, through deep RNA sequencing on human endometrial cancer (EC) samples their paired adjacent normal tissues, we uncovered circRNA...
In a wide variety of cell types, including neurons and smooth muscle cells, activation the large-conductance voltage- Ca 2+ -activated K + (BK) channels causes transient membrane hyperpolarization, thereby regulating cellular excitability. Similar to other voltage-gated ion channels, BK tetramer α-subunits, associate with auxiliary β-subunits in tissue-specific manner, modifying channel's gating properties. The β1-subunit, which is expressed muscle, increases apparent sensitivity (marked by...
Abstract Purpose: Hyperactivation of the phosphatidylinositol 3-kinase/Akt signaling through disruption PTEN function is common in glioblastoma multiforme, and these genetic changes are predicted to enhance sensitivity mammalian target rapamycin (mTOR) inhibitors such as RAD001 (everolimus). Experimental Design: To test whether loss could be used a predictive marker for mTOR inhibitor sensitivity, response 17 serially transplantable multiforme xenografts was evaluated an orthotopic therapy...
Cytosolic guanylyl cyclases (GTP pyrophosphate-lyase [cyclizing; EC 4.6.1.2]), primary receptors for nitric oxide (NO) generated by NO synthases, are obligate heterodimers consisting of an alpha and a beta subunit. The alpha1/beta1 form cyclase has the greatest activity is considered universal form. An isomer beta1 subunit, i.e., beta2, been detected in liver kidney, however, its role not known. In this study, we investigated function beta2. Immunoprecipitation experiments showed that beta2...
The regulation of Ca(2+) influx through the phosphorylation L-type channel, Ca(v)1.2, is important for modulation excitation-contraction (E-C) coupling in heart. Ca(v)1.2 thought to be target multiple kinases that mediate signals both renin-angiotensin and sympathetic nervous systems. Detailed biochemical information regarding protein reactions involved limited. kinase C (PKC) family can modulate cardiac contractility a complex manner, such either enhanced or depressed relaxation accelerated...
Advances in oral SERDs development so far have been confined to nonsteroidal molecules such as those containing a cinnamic acid moiety, which are earlystage clinical evaluation. ZB716 was previously reported an orally bioavailable SERD structurally analogous fulvestrant. In this study, we examined the binding details of estrogen receptor alpha (ERα) by computer modeling reveal its interactions with ligand domain steroidal molecule. We also found that modulates ERα-coregulator nearly...
Ovarian cancer is the most lethal gynecologic malignancy. About 75% of ovarian patients relapse and/or develop chemo-resistant disease after initial response to standard-of-care treatment with platinum-based therapies. HER2 amplifications and overexpression in are reported vary, responses inhibitors have been poor. Next generation sequencing technologies conjunction testing using patient-derived xenografts (PDX) allow validation personalized treatments. Using a whole-genome mate-pair next...
SLC7A11 is a key regulator of ferroptosis, which mediates cysteine uptake for glutathione biosynthesis and maintains redox homeostasis. Emerging evidence has shown that upregulated in many human tumors. Nevertheless, the prognosis posttranslational regulatory mechanism renal cell carcinoma (RCC) remains obscure.The Oncomine, Gene Expression Profiling Interactive Analysis (GEPIA), The Cancer Genome Atlas (TCGA) databases were used to analyze difference expression between malignant normal...
Durvalumab plus gemcitabine and cisplatin has a significant clinical benefit for advanced biliary tract cancer (BTC). However, the high price of durvalumab warrants an exploration economics.To investigate cost-effectiveness adding to compared with in first-line therapy BTC from perspective Chinese healthcare system.According TOPAZ-1 trial, three-state Markov model was built by TreeAge Pro 2022 software. The total costs quality-adjusted life years (QALYs) were estimated, incremental ratio...