A5070480124- Lung Cancer Research Studies
- Cancer Genomics and Diagnostics
- Prostate Cancer Treatment and Research
- Neuroendocrine Tumor Research Advances
- Lung Cancer Treatments and Mutations
- Molecular Biology Techniques and Applications
- Pancreatic and Hepatic Oncology Research
- Occupational and environmental lung diseases
- Nanopore and Nanochannel Transport Studies
- Cancer, Lipids, and Metabolism
- Neuroblastoma Research and Treatments
- RNA modifications and cancer
- Peptidase Inhibition and Analysis
- Renal cell carcinoma treatment
- Cancer Research and Treatments
- Ion channel regulation and function
- Cancer, Hypoxia, and Metabolism
- Advanced biosensing and bioanalysis techniques
- Genetic factors in colorectal cancer
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Ubiquitin and proteasome pathways
- Microfluidic and Bio-sensing Technologies
- Ferroptosis and cancer prognosis
- Gene expression and cancer classification
- Ion Transport and Channel Regulation
Mayo Clinic
2016-2025
Mayo Clinic in Arizona
2015-2025
WinnMed
2004-2025
Mayo Clinic in Florida
2006-2018
University of Mississippi Medical Center
2012
Purdue University West Lafayette
2004
University of Pennsylvania
1995-1999
Philadelphia VA Medical Center
1999
Single molecule sensors in which nanoscale pores within biological or artificial membranes act as mechanical gating elements are very promising devices for the rapid characterization and sequencing of nucleic acid molecules. The two terminal electrical measurements translocation polymers through single ion channels that ssDNA molecules protein have been demonstrated, sparked tremendous interest such sensors. prevailing view regarding nanopore is there exists no interaction between...
The epithelial Na<sup>+</sup> Channel (ENaC) mediates reabsorption in a variety of tissues. ENaC is composed three homologous subunits, termed α, β, and γ. All subunits participate channel formation as the absence any one subunit results significant reduction or complete abrogation current expression in<i>Xenopus</i> oocytes. To determine stoichiometry, biophysical assay was employed utilizing mutant that display differences sensitivity to blockers from wild type channel. Our indicate...
Most human tumors have abnormal numbers of chromosomes, a condition known as aneuploidy. The mitotic checkpoint is an important mechanism that prevents aneuploidy by restraining the activity anaphase-promoting complex (APC). deubiquitinase USP44 was identified key regulator APC activation; however, physiological importance and its impact on cancer biology are unknown. To clarify role in mitosis, we engineered mouse lacking Usp44. We found regulated prevented chromosome lagging. Mice Usp44...
Epigenetic modifications in eukaryotic genomes occur primarily the form of 5-methylcytosine (5 mC). These are heavily involved transcriptional repression, gene regulation, development and progression diseases including cancer. We report a new single-molecule assay for detection DNA methylation using solid-state nanopores. Methylation is detected by selectively labeling sites with MBD1 (MBD-1x) proteins, complex inducing 3 fold increase ionic blockage current relative to unmethylated DNA....
IntroductionMalignant pleural mesothelioma is a disease primarily associated with exposure to the carcinogen asbestos. Whereas other carcinogen-related tumors are high tumor mutation burden, not. We sought resolve this discrepancy.MethodsWe used mate-pair (n = 22), RNA 28), and T cell receptor sequencing along in silico predictions immunologic assays understand how structural variants of chromosomes affect transcriptome.ResultsWe observed that inter- or intrachromosomal rearrangements were...
IntroductionAlthough most patients with SCLC die within a few months of diagnosis, subgroup survive for many years. Factors determining long-term survivorship remain largely unknown. We present the first comprehensive comparative genomic and tumor microenvironment analyses between expected survivorship.MethodsWe compared surgically resected tumors 23 survivors (survival >4 years) 18 survival time ≤2 years). There were no significant differences in clinical variables, including TNM staging...
Abstract Purpose: The objective of this study was to use gene expression profiling identify novel biomarkers that are predictive aggressive behavior in clear cell renal carcinoma (CCRCC). Experimental Design: Candidate genes were discovered using Human Genome U133 Plus 2 Arrays and validated on independent samples by quantitative reverse transcription-PCR (RT-PCR). Both the discovery validation cohorts included nonaggressive primary CCRCC, metastatic nonneoplastic kidney adjacent tumor....
DNA methylation is an epigenetic modification of in which methyl groups are added at the 5-carbon position cytosine. Aberrant methylation, has been associated with carcinogenesis, can be assessed various biological fluids and potentially used as markers for detection cancer. Analytically sensitive specific assays targeting low-abundance fragmented needed optimal clinical diagnosis prognosis. We present a nanopore-based direct assay that circumvents bisulfite conversion polymerase chain...
IntroductionGenomic technologies present a promising mechanism of resolving the clinical dilemma distinguishing independent primary tumors from intrapulmonary metastases in NSCLC. We evaluated utility discordant mapping somatic junctions chromosomal rearrangements diagnosing metastatic disease compared to current standard histologic review.Material and MethodsMate-pair sequencing was performed on DNA extracted 76 distinct 37 cases multiple lung cancers. Discordant copy levels were assessed...
No AccessJournal of UrologyInvestigative Urology1 Feb 2015The Cistrome and Gene Signature Androgen Receptor Splice Variants in Castration Resistant Prostate Cancer Cells Ji Lu, Peter E. Lonergan, Lucas P. Nacusi, Liguo Wang, Lucy J. Schmidt, Zhifu Sun, Travis Van der Steen, Stephen A. Boorjian, Farhad Kosari, George Vasmatzis, G. Klee, Steven Balk, Haojie Huang, Chunxi Donald Tindall LuJi Lu Department Urology, First Hospital Jilin University, Changchun, People's Republic China Mayo Clinic,...
Abstract Purpose: Pulmonary carcinoid tumors account for up to 5% of all lung malignancies in adults, comprise 30% malignancies, and are defined histologically as typical (TC) atypical (AC) tumors. The role specific genomic alterations the pathogenesis pulmonary remains poorly understood. We sought identify pathways that deregulated these find novel therapeutic targets Experimental Design: performed integrated analysis comprising whole genome exome sequencing, mRNA expression profiling SNP...
Abstract Recently, the use of a liquid biopsy has shown promise in monitoring tumor burden. While point mutations have been extensively studied, chromosomal rearrangements demonstrated greater specificity. Such can be identified and subsequently detected plasma patients using quantitative PCR (qPCR). In this study we used whole-genome mate-pair protocol to characterize landscape genomic primary tumors ten ovarian cancer patients. Individualized tumor-specific primer panels aberrant junctions...
Tiny silicon microchip–based high-throughput hanging drop arrays allow drug screening and geometric control.
In a previous study of gene array data, the authors identified survivin as candidate marker aggressiveness in clear cell renal carcinoma (ccRCC). What remained question was whether expression at protein level is an independent predictor disease progression and cancer-specific survival.Between 1990 1994, 312 patients underwent nephrectomy for ccRCC Mayo Clinic Rochester had paraffin tissue available. The performed immunohistochemistry with antisurvivin antibody, quantitated by using...
Purpose In men who are at high-risk of prostate cancer, progression and death from cancer after radical retropubic prostatectomy (RRP), limited prognostic information is provided by established features. The objective this study was to develop a model predictive outcome in group patients. Methods Candidate genes were identified microarray expression data 102 laser capture microdissected tissue samples. Candidates overexpressed tumor compared with normal more frequently Gleason patterns 4 5...
Results from several microarray-based studies have led to the identification of up-regulated expression levels DSG3 gene in pulmonary squamous cell carcinomas (SQCCs). The purpose this study was determine role diagnosis SQCCs lung and compare with p63, CK5, CK6, as markers differentiation. Expression mRNA evaluated bulk laser capture microdissection-derived microarray data by quantitative reverse transcription PCR on both adenocarcinomas. CK6 were same set. An immunohistochemical using...
Abstract Background To discover prostate cancer biomarkers, we profiled gene expression in benign and malignant cells laser capture microdissected (LCM) from tissues metastatic prostatic adenocarcinomas. Here present methods developed, optimized, validated to obtain high quality data. Results RNase inhibitor was included solutions used stain frozen tissue sections for LCM, which improved RNA significantly. Quantitative PCR assays, requiring minimal amounts of LCM RNA, were developed...
High-throughput next-generation sequencing provides a revolutionary platform to unravel the precise DNA aberrations concealed within subgroups of tumour cells. However, in many instances, limited number cells makes application this technology heterogeneity studies challenge. In order address these limitations, we present novel methodology partner laser capture microdissection (LCM) with platforms, through whole-genome amplification (WGA) protocol performed situ directly on LCM engrafted We...
Introduction . We investigated the expression of microRNAs and mRNAs in pleural tissues from patients with either malignant mesothelioma or benign asbestos-related effusion. Methods Fresh frozen a total 18 6 effusion were studied. Expression profiling mRNA microRNA was performed using standard protocols. Results discovered significant upregulation multiple compared to Hsa-miR-484, hsa-miR-320, hsa-let-7a, hsa-miR-125a-5p able discriminate disease. Dynamically regulated also identified. MET...
Abstract Purpose: This paper describes a process for the identification of genes that can report on aggressiveness prostate tumors and thereby add to information provided by current pathologic analysis. Materials Methods: Expression profiling data from over 100 laser capture microdissection derived samples nonneoplastic epithelium; Gleason patterns 3, 4, 5 node metastasis cancer were used identify at abnormally high levels in only some tumors. These variably overexpressed stratified their...
Abstract The objective of this study was to assess the relationship tumor protein levels TOP2A and MIB-1 ERG status with cancer-specific outcomes in men high-risk prostate cancer treated by radical prostatectomy (RP). A 150-pair case-control designed from RP patients who developed systemic progression (SP) within 6 years (cases) were free disease at least 8 after (controls). cases controls matched on conventional prognostic clinical parameters. assessed immunohistochemical methods,...
Gleason score 7 (GS7) prostate cancer [tumors with both patterns 3 (GP3) and 4 (GP4)] portends a significantly more aggressive tumor than 6 (GS6). It is, therefore, critical to understand the molecular relationship of adjacent GP3 GP4 cell populations relate abnormalities disease progression. To decipher relatedness, we used laser capture microdissection (LCM) whole-genome amplification (WGA) separately collect amplify DNA from 14 cases GS7 cancer. We then carried out massively parallel...