A5047981966- Sarcoma Diagnosis and Treatment
- PARP inhibition in cancer therapy
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related molecular mechanisms research
- Lung Cancer Treatments and Mutations
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- Peptidase Inhibition and Analysis
- Pancreatic and Hepatic Oncology Research
- RNA modifications and cancer
- Cell Adhesion Molecules Research
- Cancer Immunotherapy and Biomarkers
- Molecular Biology Techniques and Applications
- Immune Cell Function and Interaction
- Ovarian cancer diagnosis and treatment
- Protein Degradation and Inhibitors
- Glycosylation and Glycoproteins Research
- Heat shock proteins research
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Virus-based gene therapy research
- Chronic Lymphocytic Leukemia Research
- Viral Infectious Diseases and Gene Expression in Insects
- HIV Research and Treatment
- Radiopharmaceutical Chemistry and Applications
Istituto Ortopedico Rizzoli
2015
The paucity of new drugs for the treatment Ewing sarcoma (EWS) limits cure these patients. CD99 has a strong membranous expression in EWS cells and, being also necessary tumor survival, is suitable target to aim at. In this article, we described novel human monospecific bivalent single-chain fragment variable diabody (dAbd C7) directed against potential clinical application.In vitro and vivo evaluation cell death molecular mechanisms triggered by anti-CD99 agents were performed alone or...
In chronic lymphocytic leukemia (CLL), natural killer (NK) cells show a dysfunctional phenotype that correlates with disease progression. Our aim was to restore NK cell functionality in CLL through specifically targeted IL15-stimulating activity; IL15 targeting could, fact, potentiate the activity of and reduce off-target effects. We designed developed cis-acting immunocytokine composed an anti-CD56 single-chain Fragment variable (scFv) IL15, labeled scFvB1IL15. scFvB1IL15 tested vitro on...
Background The mucosal tissues play a central role in the transmission of HIV-1 infection as well pathogenesis AIDS. Despite several clinical studies reported intestinal dysfunction during HIV infection, mechanisms underlying HIV-induced impairments epithelial barrier are still unclear. It has been postulated that alters enterocytic function and proteins have detected cell types mucosa. In present study, we analyzed effect accessory Nef protein on human line. Methodology/Principal Findings...
Several lines of evidence show that de novo expression carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) is strongly associated with reduced disease-free survival patients affected by metastatic melanoma. Previously published investigations report homophilic interactions between CEACAM1 expressed on natural killer (NK) cells and tumors inhibit the NK cell-mediated killing independently major histocompatibility complex class I recognition. This biological property can be...
Abstract Ewing sarcoma is the second most frequent primary tumor of bone, preferentially occurring in children and adolescents. Despite significant improvements have been achieved localized tumors thanks to dose intensification chemotherapy, outcome patients with metastasis at diagnosis remains grim. In addition, even favorable situations, must face important side effects which significantly impact their quality life. Either scientific patient communities are now very sensitive on need new...
<p>Effects of combined treatment with doxorubicin and dAbd C7.</p>
<p>p53 is necessary for CD99-induced cell-death and phosphorilated by 0662.</p>
Supplementary Methods from CD99 Triggering in Ewing Sarcoma Delivers a Lethal Signal through p53 Pathway Reactivation and Cooperates with Doxorubicin
<p>HSP70 levels are unaffected from dAbd C7 treatment.</p>
<p>Increased avidity and efficacy of dAbd over scFv C7 in 6647 EWS cell line.</p>
<p>Effects of 0662 on Gadd45-α mRNA expression and osteoblastic differentiation mesenchymal stem cells.</p>
<p>dAbd C7 activity is related to p53 status, but ineffective in h-MSC cells.</p>
<div>Abstract<p><b>Purpose:</b> The paucity of new drugs for the treatment Ewing sarcoma (EWS) limits cure these patients. CD99 has a strong membranous expression in EWS cells and, being also necessary tumor survival, is suitable target to aim at. In this article, we described novel human monospecific bivalent single-chain fragment variable diabody (dAbd C7) directed against potential clinical application.</p><p><b>Experimental...
<div>Abstract<p><b>Purpose:</b> The paucity of new drugs for the treatment Ewing sarcoma (EWS) limits cure these patients. CD99 has a strong membranous expression in EWS cells and, being also necessary tumor survival, is suitable target to aim at. In this article, we described novel human monospecific bivalent single-chain fragment variable diabody (dAbd C7) directed against potential clinical application.</p><p><b>Experimental...
<p>HSP70 levels are unaffected from dAbd C7 treatment.</p>
<p>p53 is necessary for CD99-induced cell-death and phosphorilated by 0662.</p>
<p>dAbd C7 activity is related to p53 status, but ineffective in h-MSC cells.</p>
<p>Effects of 0662 on Gadd45-α mRNA expression and osteoblastic differentiation mesenchymal stem cells.</p>
<p>Effects of combined treatment with doxorubicin and dAbd C7.</p>
Supplementary Methods from CD99 Triggering in Ewing Sarcoma Delivers a Lethal Signal through p53 Pathway Reactivation and Cooperates with Doxorubicin