A5051124744- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Heat shock proteins research
- Nuclear Structure and Function
- Angiogenesis and VEGF in Cancer
- Chromatin Remodeling and Cancer
- Hormonal and reproductive studies
- Nutrition, Genetics, and Disease
- Marine Biology and Ecology Research
- Histone Deacetylase Inhibitors Research
- Lymphatic System and Diseases
- Bee Products Chemical Analysis
- Hippo pathway signaling and YAP/TAZ
- Molecular Biology Techniques and Applications
- Effects and risks of endocrine disrupting chemicals
- Pluripotent Stem Cells Research
- Cancer-related gene regulation
- Protist diversity and phylogeny
- Microtubule and mitosis dynamics
- Environmental DNA in Biodiversity Studies
- ATP Synthase and ATPases Research
- Chemical Reactions and Isotopes
Osaka University
2023
Protein Research Foundation
2023
Nagasaki University
2015-2016
Kyushu University
2001-2006
Polydom is an extracellular matrix protein involved in lymphatic vessel development. Polydom-deficient mice die immediately after birth due to defects remodeling, but the mechanism poorly understood. Here, we report that directly binds Tie1, orphan receptor Angiopoietin–Tie axis, and facilitates migration of endothelial cells (LECs) a Tie1-dependent manner. Polydom-induced LEC diminished by PI3K inhibitors not ERK inhibitor, suggesting PI3K/Akt signaling pathway migration. In line with this...
Abstract Histone acetylation plays a pivotal role in transcriptional regulation and ATP-dependent nucleosome remodeling activity is required for optimal transcription from chromatin. While these two activities have been well characterized, how they are coordinated remains to be determined. We discovered histone H2A Drosophila nuclear extracts. This was column purified demonstrated composed of the enzymatic CREB-binding protein (CBP) SMARCAD1, which belongs Etl1 subfamily Snf2 family...
To elucidate the molecular mechanisms involved with spermiogenesis in testis, we performed differential display screening to isolate genes that are developmentally up-regulated during rat testis development. One of cDNAs isolated by was highly expressed testis. Both reverse transcription-polymerase chain reaction and Northern blot analysis showed expression level gene increased. By cDNA library, successfully clones encoding entire open-reading frame 462 base pairs coding a small protein 154...
Tektins are composed of a family filament-forming proteins associated with ciliary and flagellar microtubules. A new member the TEKTIN gene family, which was designated as rat Tektin4, obtained by PCR technique combined yeast two-hybrid screening. Rat Tektin4 cDNA consists 1,341 bp encoding 52 kDa protein 447 amino acids. contains Tektin domain including nonapeptide signature sequence (RPNVELCRD), is prominent feature Tektins. Its acid showed 29% ∼ 58% identities to that other registered in...
We examined the expression and localization of a small GTPase, rab12, in rat testis. Northern blot analysis showed that 2.3 kb transcript rab12 was expressed RT-PCR indicated constant throughout testis development. Immunohistochemical studies revealed protein highly Sertoli cells seminiferous tubules, while both spermatogenic germ interstitial exhibited faint or no immunosignal for rab12. The pattern varied between tubules: its immunostaining appeared as wheel-like at stage I approximately...
In mouse embryonic stem (mES) cells, ubiquitylation of histone H2A lysine 119 represses a large number developmental genes and maintains mES cell pluripotency. It has been suggested that ubiquitin ligases as well deubiquitylases related peptide fragments contribute to delicate balance between self-renewal multi-lineage differentiation in cells. Here, we tested whether known are involved regulation discovered Dzip3, the E3 ligase H2AK119, differentiation-inducible genes, does Ring1B. The two...
Postnatal development of mammalian seminiferous tubules can be divided into three phases: spermatogonial mitosis, spermatocyte meiosis, and a postmeiotic phase in which drastic morphological changes occur spermatids (spermiogenesis). In an attempt to elucidate the molecular mechanisms involved spermiogenesis, we have applied differential display method identify genes that are developmentally up-regulated during rat testis development. One cDNA fragments isolated by turned out iba1, ionized...
Iba1 is a 17-kDa EF-hand protein highly expressed in the cytoplasm of elongating spermatids testis. Using as bait, we performed yeast Two-hybrid screening and isolated heat-shock Hsp40, DjB1, from cDNA library mouse To characterize DjB1 that encoded by Dnajb1 gene, carried out immunoblot analyses, situ hybridization, immunohistochemistry. Immunoblot analyses showed DjB1was constitutively testis its expression level was not changed heat shock. mRNA exclusively spermatocytes round testis,...
Differential display in combination with a cDNA cloning approach were used to isolate novel gene, spergen-2, which has an open reading frame of 1500 nucleotides and encodes protein 500 amino acids that contains ankyrin repeat motifs putative nuclear localization signal. Expression spergen-2 is developmentally upregulated testis. In situ hybridization revealed mRNA expressed spermatocytes round spermatids (steps 1–6). Immunohistochemical analysis confocal laser-scanning microscopy...
Acetylation of nucleosomal histones by diverse histone acetyltransferases (HAT) plays pivotal roles in many cellular events. Discoveries novel HATs and HAT related factors have provided new insights to understand the mechanisms acetylation. In this study, we identified prominent Histone H3 acetylation activity vitro purified its activity, showing that it is composed MYST acetyltransferase Chameau Enhancer Acetyltransferase (EAChm) family. EAChm a negatively charged acidic protein retaining...
ABSTRACT: By use of differential display in combination with cDNA cloning approach, we isolated a novel rat gene designated as Spergen‐3, which has an open reading frame 2055‐length nucleotides encoding protein 685 amino acids. Spergen‐3 is composed 15 exons and mapped on chromosome 5q36, its mouse counterpart, shares 85.5% identity to at the acid level, 4E1. encodes 75‐kDa soluble bearing putative 2 EF‐hand motifs, proline‐repeat, nuclear localization signal. Of motifs second one seems...