A5094319616- Animal Genetics and Reproduction
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Retinal Development and Disorders
Ludwig-Maximilians-Universität München
2022-2025
Genomics (United Kingdom)
2025
Comparisons of molecular phenotypes across primates provide unique information to understand human biology and evolution, single-cell RNA-seq CRISPR interference (CRISPRi) screens are a powerful approach analyze them. Here, we generate validate three human, gorilla, two cynomolgus iPS cell lines that carry dox-inducible KRAB-dCas9 construct at the AAVS1 locus. We show despite variable expression levels among lines, comparable downregulation target genes phenotypic effects observed in CRISPRi...
The identification of cell types remains a major challenge. Even after decade single-cell RNA sequencing (scRNA-seq), reasonable type annotations almost always include manual non-automated steps. orthologous across species complicates matters even more, but at the same time strengthens confidence in assignment. Here, we generate and analyze dataset consisting embryoid bodies (EBs) derived from induced pluripotent stem cells (iPSCs) four primate species: humans, orangutans, cynomolgus, rhesus...
The identification of cell types remains a major challenge. Even after decade single-cell RNA sequencing (scRNA-seq), reasonable type annotations almost always include manual non-automated steps. orthologous across species complicates matters even more, but at the same time strengthens confidence in assignment. Here, we generate and analyze dataset consisting embryoid bodies (EBs) derived from induced pluripotent stem cells (iPSCs) four primate species: humans, orangutans, cynomolgus, rhesus...
Abstract The identification of cell types remains a major challenge. Even after decade single-cell RNA sequencing (scRNA-seq), reasonable type annotations almost always include manual non-automated steps. orthologous across species complicates matters even more, but at the same time strengthens confidence in assignment. Here, we generate and analyze dataset consisting embryoid bodies (EBs) derived from induced pluripotent stem cells (iPSCs) four primate species: humans, orangutans,...