A5091971303- Nuclear Structure and Function
- Research on Leishmaniasis Studies
- RNA Research and Splicing
- Malaria Research and Control
- RNA regulation and disease
- Insect symbiosis and bacterial influences
- Complement system in diseases
- Trypanosoma species research and implications
- Invertebrate Immune Response Mechanisms
Oxford Brookes University
2023-2025
The unicellular parasite Leishmania has a precisely defined cell architecture that is inherited by each subsequent generation, requiring highly coordinated pattern of duplication and segregation organelles cytoskeletal structures. A framework nuclear division morphological changes known from light microscopy, yet this limited resolution the intrinsic organisation within body their manner inheritance unknown. Using volume electron microscopy approaches, we have produced three-dimensional...
Mitosis in eukaryotes involves reorganization of the nuclear envelope (NE) and microtubule-organizing centres (MTOCs). During male gametogenesis Plasmodium, causative agent malaria, mitosis is exceptionally rapid highly divergent. Within 8 min, haploid gametocyte genome undergoes three replication cycles (1N to 8N), while maintaining an intact NE. Axonemes assemble cytoplasm connect a bipartite MTOC-containing pole (NP) cytoplasmic basal body, producing eight flagellated gametes. The...
Mitosis in eukaryotes involves reorganization of the nuclear envelope (NE) and microtubule-organizing centres (MTOCs). During male gametogenesis Plasmodium, causative agent malaria, mitosis is exceptionally rapid highly divergent. Within 8 min, haploid gametocyte genome undergoes three replication cycles (1N to 8N), while maintaining an intact NE. Axonemes assemble cytoplasm connect a bipartite MTOC-containing pole (NP) cytoplasmic basal body, producing eight flagellated gametes. The...
Mitosis in eukaryotes involves reorganization of the nuclear envelope (NE) and microtubule-organizing centres (MTOCs). During male gametogenesis Plasmodium, causative agent malaria, mitosis is exceptionally rapid highly divergent. Within 8 min, haploid gametocyte genome undergoes three replication cycles (1N to 8N), while maintaining an intact NE. Axonemes assemble cytoplasm connect a bipartite MTOC-containing pole (NP) cytoplasmic basal body, producing eight flagellated gametes. The...
Gametogenesis in Plasmodium spp. occurs within the Anopheles mosquito and is essential for sexual reproduction / differentiation onwards transmission to mammalian hosts. To better understand 3D organisation of male gametogenesis, we used serial block face scanning electron microscopy (SBF-SEM) serial-section cellular tomography (ssET) P. berghei microgametocytes examine key structures during gamete formation. Our data reveals an elaborate axonemes coiling around nucleus opposite directions...
Abstract Plasmodium spp. sexual reproduction occurs within the Anopheles mosquito and is essential for gametogenesis onwards transmission to mammalian hosts. Upon activation, male P. berghei gametocyte undergoes three rounds of inter-nuclear mitosis assembles eight basal bodies axonemes around nucleus prior ex-flagellation, resulting in 8 flagellated gametes 12-15 minutes. However there little understanding 3D organisation this rapid process gametogenesis. In study we used serial block face...
Abstract The unicellular parasite Leishmania has a precisely defined cell architecture that is inherited by each subsequent generation, requiring highly coordinated pattern of duplication and segregation organelles cytoskeletal structures. A framework nuclear division morphological changes known from light microscopy, yet this limited resolution the intrinsic organisation within body their manner inheritance unknown. Using volume electron microscopy approaches, we have produced...