ABSTRACT The level of kynurenine hydroxylase was measured throughout the development wild type and eye color mutants v, cn, st, ltd, cd, kar, w, ca, bri pP Drosophila melanogaster. In all cases except cn a bimodal distribution enzyme activity during observed. Activity is initially detectable in second instar. A maximum reached early third declines prior to puparium formation. Shortly after pupation, rises dramatically reach about five times peak larval level. Maximum persists for short time,...

Abstract The subcellular distribution of the first three enzymes in ommochrome eye pigment pathway were studied by means differential centrifugation. Tryptophan pyrrolase and kynurenine formamidase are found soluble fraction homogenate. A relatively small amount tryptophan pyrrolase, 10 to 15% total, is sedimentable. head region pupae contains approximately total activity animal. not appreciably different from rest Therefore no evidence suggests that enzyme exists a particulate form...

The study of genetic regulatory mechanisms operating in plants and animals is paramount importance contemporary biology. A precise understanding the that underlie normal cellular differentiation a prerequisite for neoplastic transformation disease. At present, we are not aware single assay system can give answers to all questions already able pose. Studies RNA synthesis valuable because they provide direct measurement transcriptional activity. But these studies remain incomplete until...

In Drosophila, multiple isoforms of alpha-glycerol-3-phosphate dehydrogenase (sn-glycerol-3-phosphate: NAD+ 2-oxidoreductase, EC 1.1.1.8) are produced in a tissue- and stage-specific manner. To understand the underlying molecular basis these isoforms, we have sequenced 5.8-kilobase region Drosophila genome that contains entire Gpdh locus. Primer-extension RNase protection assays show gene consists eight exons has single transcription-start point. mapping comparison genomic sequence from...

A laboratory strain of Drosophila virilis was genetically transformed with a hobo vector carrying the miniwhite cassette using helper plasmid an hsp70-driven transposase-coding sequence. The rate transformation 0.5% per fertile G0 animal. Three transgenic insertions were cloned and characterized found to be authentic insertions. These results, together known widespread distribution in diverse insect species, suggest that related transposable elements may considerable utility germline insects...

Abstract We have studied the Mos1 transposase encoded by transposable element mariner. This is a member of “D,D(35)E” superfamily proteins exhibiting motif D,D(34)D. It not known whether this transposase, or other eukaryote transposases manifesting D,D(35)E domain, functions in multimeric form. Evidence for oligomerization was found negative complementation an EMS-induced mutation catalytic domain. The produced has glycine-to-arginine replacement at position 292. G292R strongly interferes...

The Adh locus in Drosophila species which are members of the repleta group contains products one or two duplication events. In all examined to date genes is now a pseudogene, since mutations have rendered these incapable being translated into functional alcohol dehydrogenase. These pseudogenes contain introns standard gene position; hence, their origin not by retrotransposition. Comparison sequences Adh-psi from representatives each subgroups reveal that pseudogene present subgroup and at...

Abstract The nucleotide sequence of the Adh region Drosophila mojavensis has been completed and found to contain a pseudogene, Adh-2 Adh-1 arranged in that order. Comparison divergence these genes one another mulleri other species allowed development model for evolution duplication genes. There have two major events. An initial an gene whose dual promoter structure was similar melanogaster, resulted with genes, which may had only proximal promoter. A second this generated containing three It...

Abstract We report here the isolation and characterization of genes from Drosophila that encode glycolytic enzyme phosphoglyceromutase (PGLYM). Two genomic regions have been isolated potential to PGLYM. Their cytogenetic localizations determined by in situ hybridization salivary gland chromosomes. One gene, Pglym78, is found at 78A/B other, Pglym87, 87B4,5 polytene map. Pglym78 transcription follows a developmental pattern similar other Drosophila, i.e., substantial maternal transcript...

ABSTRACT Drosophila mojavensis and other species of the mulleri subgroup contain a duplicate gene encoding enzyme alcohol dehydrogenase (ADH). Studies on genetic relationship two genes using electrophoretic variants show them to be closely linked. We have cloned 13.5-kb fragment D. DNA into lambda vector, Charon 30. This contains both Adh separated by approximately 2 kb DNA. The clone hybridized single position chromosome 3 in following situ hybridization. It is likely that are tandemly...

The enzyme glyceraldehyde-3-phosphate dehydrogenase from Drosophila melanogwter has been purified, and these preparations contain two subunits forms which have molecular weights of 37,000 35,500, respectively.Each subunit is found in crude extracts, activity bands are seen nondenaturing acrylamide gels.Translation poly(A)-containing RNA results products precipitable with ~ti-glyceraldehyde-3-phosphate serum.Two recombinant DNA clones isolated a genomic library DNA.Each the ability to hybrid...