A5068013526- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Plant biochemistry and biosynthesis
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Mycorrhizal Fungi and Plant Interactions
- Chemical synthesis and alkaloids
- Phytochemical Studies and Bioactivities
- Carbohydrate Chemistry and Synthesis
- Synthetic Organic Chemistry Methods
- Plant and animal studies
- Plant tissue culture and regeneration
- Plant Disease Resistance and Genetics
- Legume Nitrogen Fixing Symbiosis
- Marine Sponges and Natural Products
- Plant Parasitism and Resistance
- Photosynthetic Processes and Mechanisms
- Microbial Metabolites in Food Biotechnology
Hokkaido University
2018-2025
Abscisic acid (ABA) is a well-known phytohormone that regulates abiotic stresses. ABA produced by fungi also proposed to be virulence factor of fungal pathogens. Although its biosynthetic pathway in was series feeding experiments, the enzyme catalyzing reaction from farnesyl diphosphate α-ionylideneethane remains identified. In this work, we identified novel type sesquiterpene synthase BcABA3 and unprecedented three-step mechanism involving two neutral intermediates, β-farnesene...
Plant-associated fungi show diverse lifestyles from pathogenic to mutualistic the host; however, principles and mechanisms through which they shift require elucidation. The root fungus Colletotrichum tofieldiae (Ct) promotes Arabidopsis thaliana growth under phosphate limiting conditions. Here we describe a Ct strain, designated Ct3, that severely inhibits plant growth. Ct3 pathogenesis occurs activation of host abscisic acid pathways via fungal secondary metabolism gene cluster related...
Abscisic acid (ABA) is one of the plant hormones that regulates physiological functions in various organisms, including plants, sponges, and humans. The biosynthetic machinery plants firmly established, while fungi still unclear. Here, we elucidated four genes, bcABA1-bcABA4, found Botrytis cinerea by performing biotransformation experiments vitro enzymatic reactions with putative intermediates. first-committed step cyclization farnesyl diphosphate to give α-ionylideneethane catalyzed a...
Abstract Highly reducing polyketide synthases (HR‐PKSs) produce structurally diverse polyketides (PKs). The PK diversity is constructed by a variety of factors, including the β‐keto processing, chain length, methylation pattern, and relative absolute configurations substituents. We examined stereochemical course processing for synthesis polyhydroxy PKs such as phialotides, phomenoic acid, ACR‐toxin. Heterologous expression HR‐PKS gene, trans‐acting enoylreductase truncated non‐ribosomal...
Eremophilanes exhibit diverse biological activities and chemical structures. This study reports the bioinformatics-guided reconstitution of biosynthetic machinery fungal eremophilanes, eremofortin C sporogen-AO1, to elucidate their pathways. Their biosyntheses include P450-catalyzed multistep oxidation enzyme-catalyzed isomerization by DUF3237 family protein. Successful characterization six P450s enabled us discuss functions eremophilane in putative gene clusters, providing opportunities...
Antihypercholesterolemic agent phomoidride (PMD) B has a highly elaborated bicyclo[4.3.1]deca-1,6-diene core scaffold derived from dimeric anhydride with nine-membered ring. This report elucidated the late stage transformation an monomer to PMD through heterologous expression of three enzyme genes, TstC, TstK, and TstE. Additional in vitro studies TstK TstE provided evidence on formation via dimerization, three-step oxidation, unusual methylation-triggered bicyclic ketal formation....
Abstract The infection strategies employed by plant endophytes are attributed to their ability overcome durable nonhost resistance and adapt the host environment. However, regulatory genetic background underlying how they determine lifestyles remains enigmatic. Here, we show that CtBOT6 , a cluster-residing transcription factor in root-associated fungus Colletotrichum tofieldiae (Ct), plays pivotal role regulating virulence-related gene expression producing metabolites both not only within...
ABSTRACT Previously, we succeeded to produce the core structure of host-selective ACR toxin (1) on brown leaf spot rough lemon when polyketide synthase ACRTS2 gene was heterologously expressed in Aspergillus oryzae (AO). To confirm production 1 AO, detection limit and suppressing decarboxylation were improved, these efforts led us conclude direct instead its product. During this examination, minor ACR-toxin-related metabolites found. Their determination enabled propose a mechanism novel...
Abstract Highly reducing polyketide synthases (HR‐PKSs) produce structurally diverse polyketides (PKs). The PK diversity is constructed by a variety of factors, including the β‐keto processing, chain length, methylation pattern, and relative absolute configurations substituents. We examined stereochemical course processing for synthesis polyhydroxy PKs such as phialotides, phomenoic acid, ACR‐toxin. Heterologous expression HR‐PKS gene, trans‐acting enoylreductase truncated non‐ribosomal...