Mutants in the structural gene of inhA-encoded NADH-dependent 2-trans enoyl-acyl carrier protein reductase were identified from isoniazid-resistant clinical isolates Mycobacterium tuberculosis. Recombinant InhA proteins with defined single amino acid replacements expressed Escherichia coli and purified to homogeneity. Steady-state kinetic parameters for wild type (WT) I16T, I21V, I47T, I95P mutants enoyl measured spectrophotometrically. NADH binding WT S94A, mutant reductases determined by...

Abstract Candidatus Izemoplasma, an intermediate in the reductive evolution from Firmicutes to Mollicutes, was proposed represent a novel class of free-living wall-less bacteria within phylum Tenericutes. Unfortunately, paucity pure cultures has limited further insights into their physiological and metabolic features as well ecological roles. Here, we report first successful isolation Izemoplasma representative deep-sea methane seep, strain zrk13, using DNA degradation-driven method given...

Mercury-mediated toxicity remains one of the greatest barriers against microbial survival, even though bacterial resistance to mercury compounds can occur. However, genetic and physiological adaptations bacteria stress still unclear. Here, we show that marine bacterium Pseudomonas stutzeri 273 is resistant 50 μM Hg2+ removes up 94% from culture. Using gene homologous recombination complementation, genes encoding Hg2+-transport proteins MerT, MerP, mercuric reductase MerA regulatory protein...

Summary Bacteroidetes are thought to be specialized for the degradation of algae‐derived ocean polysaccharides. Here, we show that predominant phylum in deep‐sea sediments and possess more genes associated with polysaccharides than other bacteria. We have isolated a novel species from by using special polysaccharide containing medium, Maribellus comscasis WC007, which possesses 82 putative utilization loci (PULs) 374 glycoside hydrolases SusC/D pairs (Sus indicates s tarch u tilization...

Wall-less bacteria are broadly distributed in diverse habitats. They evolved from a common ancestor within the Firmicutes phylum through reductive evolution. Here, we report cultivation, characterization, and polyphasic taxonomic analysis of novel free-living wall-less bacterium, Hujiaoplasma nucleasis zrk29. We demonstrated that strain zrk29 had strong ability to degrade DNA RNA both under laboratory conditions deep sea. found nucleic acids induced release chronic bacteriophages which...

Plant disease caused by pathogenic fungi is one of the most devastating diseases, which affects food safety whole world to a great extent. Biological control plant diseases microbial natural products more desirable than traditional chemical control. In this study, we discovered novel lipopeptide, iturin W, with promising prospects in biological diseases. Moreover, effects different carbon and nitrogen sources amino acids on production C 14 W 15 would provide reasonable basis for optimization...

To date, only three biotic pathways for light-independent oxygen (i.e. dark oxygen) production: chlorate dismutation, nitric oxide and methanobactin-dependent lysis of water. Oxygen has been shown to be produced consumed in apparently anoxic environments, as evidenced by its prevalence deep-sea surface sediments. However, the microbial communities driving production (DOP) remain unclear. Here we identified a novel DOP pathway driven process dissimilatory nitrate reduction ammonium (DNRA) two...

Methanogenic archaea play a critical role in methane (CH4) production and the global carbon cycle, yet accurately monitoring their gas metabolism under anaerobic conditions remains technical challenge. In this study, we developed Raman spectroscopy-based quantification model, achieving high-precision of CO2-N2-CH4 ternary mixtures over temperature range 12-52 °C. The model exhibited strong linear correlations between peak area ratios molar ratios, which were further validated against...

Viruses exert a pervasive influence on biogeochemical cycles in deep-sea ecosystems. By integrating multi-omics and viral isolation data from four distinct sediment sites, we uncover the diversity of DNA RNA communities, which vary significantly across geographically separated cold seep seamount environments. Host range predictions reveal both prokaryotic eukaryotic communities are susceptible to infection. Metatranscriptomic analyses suggest functional genes actively expressed, potentially...

<title>Abstract</title> Viruses exert a pervasive influence on biogeochemical cycles in deep-sea ecosystems. By integrating multi-omics and viral isolation data from four distinct sediment sites, we uncover the diversity of DNA RNA communities, which vary significantly across geographically separated cold seep seamount environments. Host range predictions reveal both prokaryotic eukaryotic communities are susceptible to infection. Metatranscriptomic analyses suggest functional genes actively...

ABSTRACT Several key virulence factors of Pseudomonas aeruginosa are regulated by quorum-sensing systems, small noncoding RNAs (sRNAs), and environmental stress, leading to a high mortality rate. Our previous studies indicated that the alkaline phosphatase LapA P. PAO1 biofilm formation in chronic wound model established with ex vivo porcine skin explants. Notably, one particular sRNA located upstream lapA gene was highly expressed model. Therefore, further characterized via northern...

<title>Abstract</title> Methanogenesis has traditionally been associated with a limited range of low-molecular-weight (LMW) substrates (MW &lt; 1,000 Da), including CO₂, acetate, methylated compounds (e.g., methanol, methylamines), methoxylated aromatic compounds, and alkanes. However, the direct utilization high-molecular-weight (HMW) organic matter &gt; such as polysaccharides, for methane production by methanogens not reported. Here, we show that novel deep-sea methanogenic...

ABSTRACT Chloroflexota bacteria are abundant and globally distributed in various deep-sea ecosystems. It has been reported based on metagenomics data that two lineages (the SAR202 group Dehalococcoidia class) have the potential to drive sulfur cycling. However, absence of cultured representatives is a significant bottleneck toward understanding their contribution In this study, we find Phototrophicus methaneseepsis ZRK33 isolated from sediment heterotrophic lifestyle can assimilate sulfate...

The deep ocean microbiota represents the unexplored majority of global waters. phylum Chloroflexi is abundant and broadly distributed in various deep-sea ecosystems.

Under multiple stresses of deep sea, many microorganisms have evolved potentials to produce different metabolites cope with the they face. In this study, we isolated a bacterial strain Bacillus sp. YJ17 from deep-sea cold seep. Compared commercial food preservative nisin, it showed broad and strong antibacterial activities against foodborne pathogens, including resistant bacteria Pseudomonas aeruginosa PAO1 methicillin-resistant Staphylococcus aureus (MRSA). The active agents were purified...

Planctomycetes bacteria are ubiquitously distributed across various biospheres and play key roles in global element cycles. However, few deep-sea members have been cultivated, limiting our understanding of the deep biosphere. Here, we successfully cultured a novel strain (strain ZRK32) from cold seep sediment. Our genomic, physiological, phylogenetic analyses indicate that ZRK32 is species, which propose be named: Poriferisphaera heterotrophicis . We show replicates using budding mode...

Sulfur cycling is primarily driven by sulfate reduction mediated sulfate-reducing bacteria (SRB) in marine sediments. The dissimilatory drives the production of enormous quantities reduced sulfide and thereby formation highly insoluble metal sulfides Here, a novel bacterium designated

Summary Cadmium (Cd) is a common toxic heavy metal in the environment, and bacteria have evolved different strategies against Cd‐toxicity. Here, we found that marine bacterium Bacillus sp. 98 could significantly alleviate Cd‐toxicity by recruiting calcium (Ca) for reducing excessive intracellular nitric oxide (NO) enhancing iron acquisition. To investigate underlying mechanisms, mass spectrometry‐based proteomic analysis was applied to after treated with Cd supplemented or without Ca....

Light is a ubiquitous energy source and environmental signal that broadly impacts the lifestyle of large number photosynthetic/nonphotosynthetic microorganisms living in euphotic layer. However, responses deep-sea microbes to light are largely unknown, even though blue proposed be distributed deep ocean. Here, we successfully cultured novel bacterial species, named Spongiibacter nanhainus CSC3.9, from cold seep samples by induction approach. The growth strain CSC3.9 was obviously promoted...

MerF, a proposed bacterial mercury transporter, was surprisingly found to play key roles in the flagellum biogenesis and motility but not mercuric resistance of deep-sea bacterium Pseudomonas stutzeri 273 our previous study. However, mechanism behind this interesting discovery has been elucidated. Here, we firstly applied combined transcriptomic proteomic analysis P. wild type merF deletion mutant. The results showed that expressions extracellular flagellar components FliS, factor...

Pseudomonas stutzeri 273 is a marine bacterium producing exopolysaccharide (EPS273) with high anti-biofilm activity against P. aeruginosa PAO1. Here, the complete genome of P.stutzeri was sequenced and contained circular 5.03 Mb chromosome. With extensive analysis genome, genetic locus containing 18 genes predicted to be involved in biosynthesis EPS273. In order confirm this prediction, two adjacent (eps273-H eps273-I) encoding glycosyltransferases one gene (eps273-O) tyrosine protein kinase...

The deep ocean microbiota has unexplored potential to provide enzymes with unique characteristics. In order obtain cold-active lipases, bacterial strains isolated from the sediment of deep-sea cold seep were screened, and a novel strain gcc21 exhibited high lipase catalytic activity, even at low temperature 4 °C. was identified proposed represent new species Pseudomonas according its physiological, biochemical, genomic characteristics; it named marinensis. Two encoding genes for lipases...

As a member of Planctomycetes – Verrucomicrobia Chlamydia (PVC) superphylum, the phylum Lentisphaerae is broadly distributed in various environments. members have been recognized as being typically difficult to isolate pure culture—only five strains belonging this were cultured up now—and therefore their major physiology and ecology are largely unknown. Here, using polysaccharide degradation-driven strategy, we successfully representative bacteria ( Candidatus Coldseepensis marina zth2) from...

A Gram-stain-negative, facultatively anaerobic, yellow-pigmented, non-motile, rod-shaped bacterium, designated zrk23T, was isolated from a deep-sea cold seep. The strain characterized by polyphasic approach to clarify its taxonomic position. Phylogenetic analysis based on 16S rRNA gene sequences placed zrk23T within the genus Sphingosinithalassobacter and showed highest similarity portus FM6T (97.93 %). Growth occurs at temperatures 16 45 °C (optimum, 30 °C), pH values between 6.0 8.5 7.0)...

Relatively, few anti‐biofilm polysaccharides against Pseudomonas aeruginosa were done to investigate the underlying molecular mechanism. Exopolysaccharide EPS273 can clearly reduce biofilm formation and infection of P. aeruginosa. This study aims its anti‐infection mechanism on transcriptional level. Herein, we used an RNA‐Seq transcriptomic approach EPS273. The expression levels a large number genes changed after PAO1 was treated with Especially, related formation, such as gene involved in...