A5051004969- Single-cell and spatial transcriptomics
- Cancer Genomics and Diagnostics
- Acute Myeloid Leukemia Research
- Bacterial biofilms and quorum sensing
- Polysaccharides and Plant Cell Walls
- Cell Image Analysis Techniques
- Advanced Cellulose Research Studies
- Food composition and properties
- Biosensors and Analytical Detection
- Electrohydrodynamics and Fluid Dynamics
- Oral microbiology and periodontitis research
- Lignin and Wood Chemistry
- Biofuel production and bioconversion
- Advanced NMR Techniques and Applications
- Wood Treatment and Properties
- Fluid Dynamics and Heat Transfer
- Microbial Community Ecology and Physiology
- Surface Modification and Superhydrophobicity
- Bacteriophages and microbial interactions
Ningbo University
2024-2025
Fluent (United States)
2022-2024
Selecta Biosciences (United States)
2022-2024
State Key Laboratory of Pollution Control and Resource Reuse
2024
Nanjing University
2024
Current single-cell RNA-sequencing approaches have limitations that stem from the microfluidic devices or fluid handling steps required for sample processing. We develop a method does not require specialized devices, expertise hardware. Our approach is based on particle-templated emulsification, which allows encapsulation and barcoding of cDNA in uniform droplet emulsions with only vortexer. Particle-templated instant partition sequencing (PIP-seq) accommodates wide range emulsification...
The complex and dynamic compositions of biofilms, along with their sophisticated structural assembly mechanisms, endow them exceptional capabilities to thrive in diverse conditions that are typically unfavorable for individual cells. Characterizing biofilms native state is significantly challenging due intrinsic complexities the limited availability noninvasive techniques. Here, we utilized solid-state nuclear magnetic resonance (NMR) spectroscopy analyze Bacillus subtilis in-depth. Our data...
Dispersal plays a crucial role in the development and ecology of biofilms. While extensive studies focused on elucidating molecular mechanisms governing this process, few have characterized associated temporal changes composition structure. Here, we employed solid-state nuclear magnetic resonance (NMR) techniques to achieve time-resolved characterization Bacillus subtilis biofilms over 5-day period. The mature biofilm, established within 48 h, undergoes significant degradation following 72...
A major factor limiting bark's industrial use is its greater recalcitrance compared to wood. While lignin widely recognized as a significant contributor, precise characterization of in bark remains sparse, presenting crucial gap that impedes understanding impact. In this study, we employed advanced solid-state nuclear magnetic resonance (NMR) spectroscopy analyze samples from various species, including willow, poplar, and pine. We established verified methoxy peak at 56 ppm serves reliable...
Abstract Single-cell RNA sequencing is now a standard method used to reveal the molecular details of cellular heterogeneity, but current approaches have limitations on speed, scale, and ease use that stem from complex microfluidic devices or fluid handling steps required for sample processing. We, therefore, developed does not require specialized devices, expertise, hardware. Our approach based particle-templated emulsification, which allows single-cell encapsulation barcoding cDNA in...
Xylan, a pivotal polymer with diversified structures, is indispensable for cell wall integrity and contributes to plant growth biomass recalcitrance. Xylan synthesized by multi-enzyme complexes named xylan synthase (XSCs). However, the biochemical mechanism of XSCs functions core components within XSC remain unclear. Here, we report that rice (Oryza sativa) XYLAN O-ACETYLTRANSFERASE 6 (XOAT6) IRREGULAR XYLEM10 (IRX10) represent XSC, acting together biosynthesize acetyl-xylans....
Mixed phenotype acute leukemia (MPAL) is a whose biologic drivers are poorly understood, therapeutic strategy remains unclear, and prognosis poor. We performed multiomic single cell (SC) profiling of 14 newly diagnosed adult MPAL patients to characterize the immunophenotypic, genetic, transcriptional landscapes MPAL. show that neither genetic profile nor transcriptome reliably correlate with specific immunophenotypes. However, progressive acquisition mutations associated increased expression...