All-optical cardiac electrophysiology enables the visualization and control of key parameters relevant to detection arrhythmias. Mapping such responses in human induced pluripotent stem-cell-derived cardiomyocytes (hiPSC-CMs) is great interest for cardiotoxicity personalized medicine applications.

Uncovering gene-phenotype relationships can be enabled by precise gene modulation in human induced pluripotent stem-cell-derived cardiomyocytes (iPSC-CMs) and follow up phenotyping using scalable all-optical electrophysiology platforms. Such efforts towards functional genomics aided recent CRISPR-derived technologies for reversible inhibition or activation (CRISPRi/a). We set out to characterize the performance of CRISPRi post-differentiated iPSC-CMs, targeting key cardiac ion channel genes,...

The Halbach array, originally developed for particle accelerators, is a compact arrangement of permanent magnets that creates well-defined magnetic fields without heating. Here, we demonstrate its use modulating the speed electromechanical waves in cardiac syncytia human stem cell-derived cardiomyocytes. At 40-50 mT field strength, cylindrical dipolar array boosted conduction velocity, CV, by up to 25% when was co-aligned with wave (but not perpendicular it). To observe effects, short-term...

We present a simple low-cost system for comprehensive functional characterization of cardiac function under spontaneous and paced conditions, in standard 96 384-well plates. This full-plate actuator/imager, OptoDyCE-plate, uses optogenetic stimulation optical readouts voltage calcium (parallel recordings from up to 100 wells plates are demonstrated). The is validated with syncytia human induced pluripotent stem cell derived cardiomyocytes, iPSC-CMs, grown as monolayers, or quasi-3D isotropic...

Coarse mode atmospheric aerosol particles are abundant in agricultural, desert, and urban environments. Accurate characterisation of these particles' morphology is an important need scientific applied contexts, especially to advance our understanding for how such aerosols influence solar radiative forcing the atmosphere. Elastic light scattering a standard method study contact-free manner, wherein measured patterns interpreted infer particle morphology. Due part absence wave-phase...

Q-space analysis is applied to the light scattering phase function of a wide variety non-spherical and irregularly shaped particles including great many types dusts, fractal aggregates, spheroids, irregular spheres, Gaussian random thickened clusters nine ice crystals. The functions were either experimental data or calculations. This method uncovers specific quantitative similarities differences between by various shapes also when compared spheres. From this general description for particle...

Abstract We report Q‐space analysis of light scattering phase function data for irregularly shaped dust particles and theoretical model output to describe them. This involves plotting the scattered intensity versus magnitude wave vector q = (4 π / λ )sin( θ /2), where is optical wavelength angle, on a double‐logarithmic plot. In q‐space all particle shapes studied display pattern which includes q‐independent forward regime; crossover, Guinier regime when near inverse size; power law an...

In this work, approximate three-dimensional structures of microparticles are generated with digital holography using an automated focus method. This is done by stacking a collection silhouette-like images particle reconstructed from single in-line hologram. The method enables estimation the size in longitudinal and transverse dimensions. Using discrete dipole approximation, tested computationally simulating holograms for variety particles attempting to reconstruct known structure. It found...

The similarity between the light-scattering pattern of a particle in near-forward direction and diffraction from particle's silhouette is investigated. Images irregularly shaped free-flowing aerosol particles are obtained digital hologram measurements, which then binarized to yield silhouette. Application Huygens's principle generates an approximate scattering pattern, when compared true measured shows good agreement for much larger than wavelength light.

Aerosol gels are volume-spanning, semi-rigid networks of solid nanoparticles possessing ultralow density and high surface area (Dhaubhadel et al. 2007; Chakrabarty 2014). The current consens...

Abstract We show that zinc finger imprinted 3 (Zim3), when used as Zim3-KRAB-dCas9 effector in interference CRISPR, without any guide RNAs, paradoxically up-regulates key cardiac ion channel genes human-induced pluripotent stem-cell-derived cardiomyocytes (iPSC-CMs), responsible for healthy resting membrane potential, repolarization of the action and electrical transmission signals. These were found to yield expected functional enhancements consistent with a more mature iPSC-CM phenotype,...

An apparatus was designed and assembled to measure scattered light in the range of 180°±6° where enhanced backscattering, cause a glory, occurs. The calibrated tested using Fraunhofer circular aperture diffraction, angle incidence correction, diffuse reflector. Theory indicates that backscattering is strongly dependent on particle size, refractive index, shape. Experimental measurements from polystyrene latex spheres two sizes water droplets showed good agreement with Mie theory, but also...

We present a simple low-cost system for comprehensive functional characterization of cardiac function under spontaneous and paced conditions, in standard 96 384-well plates. This full-plate actuator/imager, OptoDyCE-plate, uses optogenetic stimulation optical readouts voltage calcium from all wells parallel. The is validated with syncytia human induced pluripotent stem cell derived cardiomyocytes, iPSC-CMs, grown as monolayers, or quasi-3D isotropic anisotropic constructs using electrospun...

Uncovering gene-phenotype relationships can be enabled by precise gene modulation in human induced pluripotent stem-cell-derived cardiomyocytes (iPSC-CMs) and follow up phenotyping using scalable all-optical electrophysiology platforms. Such efforts towards functional genomics aided recent CRISPR-derived technologies for reversible inhibition or activation (CRISPRi/a). We set out to characterize the performance of CRISPRi post-differentiated iPSC-CMs, targeting key cardiac ion channel genes,...

Abstract The Halbach array, originally developed for particle accelerators, is a compact arrangement of permanent magnets to create well-defined magnetic fields without heating. Here, we demonstrate its use modulating the speed electromechanical waves in cardiac syncytia human stem cell-derived cardiomyocytes. At 40-50 mT field strength, cylindrical dipolar array boosted conduction velocity, CV, excitation directional manner by up 25% when was co-aligned with wave (but not perpendicular it)....

Non-spherical aerosols, particularly aggregates and those comprised of rough surfaces, produce complex light scattering patterns that deviate considerably from their spherical counterparts. Consequently, discerning particle morphology the pattern, i.e., inverse problem, is difficult at best. Additional information required to associate uniquely interference pattern resulting scattered particle's (size, shape, etc.). This uniqueness challenge problem may be overcome by incorporating digital...

Abstract We show that Zim3, when used as Zim3-KRAB-dCas9 effector in interference CRISPR, without any guide RNAs, paradoxically upregulates key cardiac ion channel genes human induced pluripotent stem-cell-derived cardiomyocytes, iPSC-CMs, responsible for healthy resting membrane potential, repolarization of the action potential and electrical transmission signals. These were found to yield expected functional enhancements consistent with a more mature iPSC-CM phenotype, potentially...

Accurate characterization of the asymmetry parameter g is crucial importance for radiative transfer calculations. Here, we present a portable light scattering (PLS) device designed in situ, real-time, and contact-free measurements particle phase function. The integration time 20ms each measurement. Using this device, measured values laboratory-generated brown (organic) carbon aerosol soot, commercially available Arizona Road Dust. For three types aerosol, were 0.664 ± 0.002, 0.506 0.004,...