A5015282605- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- RNA Research and Splicing
- Chemical Synthesis and Analysis
- DNA and Nucleic Acid Chemistry
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Toxin Mechanisms and Immunotoxins
- Viral Infections and Immunology Research
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Mass Spectrometry Techniques and Applications
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Advanced biosensing and bioanalysis techniques
- Viral gastroenteritis research and epidemiology
- Origins and Evolution of Life
- bioluminescence and chemiluminescence research
- Biopolymer Synthesis and Applications
- Radiopharmaceutical Chemistry and Applications
- Transgenic Plants and Applications
- Insect and Pesticide Research
- RNA Interference and Gene Delivery
Moscow State University of Civil Engineering
2023
S. P. Korolev Rocket and Space Corporation Energia (Russia)
2022
Institute of Protein Research
2007-2018
Lomonosov Moscow State University
1974-2018
Russian Academy of Sciences
2000-2013
Engelhardt Institute of Molecular Biology
2009
California Institute of Technology
2008
Novartis (Switzerland)
2002
Institute of Bioorganic Chemistry
1995-1999
Institute of Theoretical and Experimental Biophysics
1998
A cell-free translation system has been constructed that uses a continuous flow of the feeding buffer [including amino acids, adenosine triphosphate (ATP), and guanosine (GTP)] through reaction mixture removal polypeptide product. Both prokaryotic (Escherichia coli) eukaryotic (wheat embryos, Triticum sp.) versions have tested. In both cases proven active for long times, synthesizing polypeptides at high constant rate tens hours. With use MS2 phage RNA or brome mosaic virus 4 as templates,...
Three structures containing messenger RNA can be demonstrated in the cytoplasm of early sea-urchin embryos: (i) particles that sediment more slowly than ribosomes and contain newly synthesized DNA-like RNA, (ii) light polyribosomes, which also this (iii) heavy seemingly only already existing or "maternal" account for bulk synthesis protein.
In attempting to picture the mechanism of ribosome participation in progressive growth polypeptide chain, a hypothesis was suggested (Spirin, 1968a, b) based on idea strict division labor between two ribosomal subparticles. The large subparticle (50 S) seen as "polymer carrier" retaining growing peptide process synthesis, while small (30 considered mobile "porter" new aminoacyl-tRNAs.
Globin synthesis in a wheat germ cell-free translation system was performed the presence of [3H]hemin and [35S]methionine to determine minimal length nascent ribosome-bound globin chain capable heme binding. Nascent polypeptides predetermined size were synthesized on ribosomes by truncated mRNA molecules. Analysis with use sucrose gradient centrifugation puromycin reaction revealed that N-terminal α-globin fragments 140, 100, 86 amino acid residues are an efficient binding, whereas those 75,...
Surface labeling of Escherichia coli ribosomes with the use tritium bombardment technique has revealed a minor unidentified ribosome-bound protein (spot Y) that is hidden in 70S ribosome and becomes highly labeled on dissociation into subunits. In present work, N-terminal sequence Y was determined its gene identified as yfia , an ORF located upstream phe operon E. . This 12.7-kDa isolated characterized. An affinity purified for 30S subunit, but not 50S ribosomal shown. The proved to be...
ABSTRACT The genomic RNA of tobacco mosaic virus (TMV), like that other positive-strand viruses, acts as a template for both translation and replication. highly structured 3′ untranslated region (UTR) TMV RNAs plays an important role in processes; it is not polyadenylated but ends with tRNA-like structure (TLS) preceded by conserved upstream pseudoknot domain (UPD). TLS tobamoviral can be specifically aminoacylated and, this state, interact eukaryotic elongation factor 1A (eEF1A)/GTP high...