A5042945411- SARS-CoV-2 detection and testing
- Advanced Chemical Sensor Technologies
- Gene expression and cancer classification
- COVID-19 diagnosis using AI
- Molecular Biology Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Bacteriophages and microbial interactions
- Protist diversity and phylogeny
- Viral gastroenteritis research and epidemiology
- Microfluidic and Capillary Electrophoresis Applications
- Biosensors and Analytical Detection
- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Plant Pathogens and Resistance
- Advanced Proteomics Techniques and Applications
- Open Education and E-Learning
- DNA and Biological Computing
- Microbial Community Ecology and Physiology
- Modular Robots and Swarm Intelligence
- Mycobacterium research and diagnosis
- Potato Plant Research
- Bacterial Genetics and Biotechnology
- Plant nutrient uptake and metabolism
Millennium Institute for Integrative Biology
2018-2024
Pontificia Universidad Católica de Chile
2018-2024
Millennium Science Initiative
2021-2024
Universidad Andrés Bello
2023-2024
University of Buenos Aires
2020
Summary High‐efficiency methods for DNA assembly have enabled the routine of synthetic s increased size and complexity. However, these techniques require customization, elaborate vector sets or serial manipulations different stages assembly. We developed Loop based on a recursive approach to fabrication. The system makes use two Type IIS restriction endonucleases corresponding efficient parallel large circuits. Standardized level 0 parts can be assembled into circuits containing 1, 4, 16...
Abstract The technique RT-qPCR for viral RNA detection is the current worldwide strategy used early of novel coronavirus SARS-CoV-2. extraction a key pre-analytical step in RT-qPCR, often achieved using commercial kits. However, magnitude COVID-19 pandemic causing disruptions to global supply chains by many diagnostic laboratories procure kits required extraction. Shortage these essential reagents even more acute developing countries with no means produce locally. We sought find an...
Abstract Standardized type IIS DNA assembly methods are becoming essential for biological engineering and research. These widespread more accessible due to the proposition of a ‘common syntax’ that enables higher interoperability between libraries. Currently, Golden Gate (GG)-based systems, originally implemented in host-specific vectors, being made compatible with multiple organisms. We have recently developed GG-based Loop system plants, which uses small library an intuitive strategy...
The COVID-19 pandemic has resulted in millions of deaths globally, and while several diagnostic systems were proposed, real-time reverse transcription polymerase chain reaction (RT-PCR) remains the gold standard. However, reagents, including enzymes used RT-PCR, are subject to centralized production models intellectual property restrictions, which present a challenge for less developed countries. With aim generating standardized One-Step open RT-qPCR protocol detect SARS-CoV-2 RNA clinical...
Tomato (Solanum lycopersicum) is a globally important crop, yet the gene regulatory networks (GRNs) controlling expression remain poorly understood. In this study, we constructed GRNs for roots, leaves, flowers, fruits, and seeds by inferring transcription factor (TF)–target interactions from over 10,000 RNA-seq libraries using GENIE3 algorithm. We refined these with co-expression data computational predictions of TF binding sites. Our confirmed key regulators, including TOMATO AGAMOUS LIKE...
Abstract Open Educational Resources (OER), freely accessible learning, teaching and research materials, have been proposed as key enabling tools to achieve inclusive knowledge societies equitable access education. Here, we describe novel OER consisting of low cost locally produced public domain biological reagents, open source hardware free software collaborative notebooks teach LAMP DNA amplification, RT-PCR RNA detection, enzyme kinetics fluorescence imaging. These resources distributed...
ABSTRACT Standardised Type IIS DNA assembly methods are becoming essential for biological engineering and research. Although a ‘common syntax’ has been proposed to enable higher interoperability between libraries, Golden Gate (GG)-based systems remain specific target organisms. Furthermore, these GG become laborious unnecessarily complicated beyond the of 4 transcriptional units. Here, we describe “universal Loop” (uLoop) assembly, simple system based on Loop that enables hierarchical...
Abstract High efficiency methods for DNA assembly are based on sequence overlap between fragments or Type IIS restriction endonuclease cleavage and ligation. These have enabled routine of synthetic DNAs increased size complexity. However, these techniques require customisation, elaborate vector sets serial manipulations the different stages assembly. We present Loop assembly, a recursive approach to fabrication. Alternate use two endonucleases corresponding allows efficient parallel large...
Abstract The technique RT-qPCR for viral RNA detection is the current worldwide strategy used early of novel coronavirus SARS-CoV-2. extraction a key pre-analytical step in RT-qPCR, often achieved using commercial kits. However, magnitude COVID-19 pandemic causing disruptions to global supply chains by many diagnostic laboratories procure kits required extraction. Shortage these essential reagents even more acute developing countries with no means produce locally. We sought find an...
This protocol has been optimized for the recombinant expression of a codon-optimized Bst-LF polymerase. The goal this was to eliminate use large volumes dyalisis through concentrators buffer exchange before storage conditions. plasmid encoding enzyme used here can be found on reclone.org
The COVID-19 pandemic has resulted in millions of deaths globally, and while several diagnostic systems were proposed, real-time reverse transcription polymerase chain reaction (RT-PCR) remains the gold standard. However, reagents, including enzymes used RT-PCR, are subject to centralized production models intellectual property restrictions, which present a challenge for less developed countries. With aim generating standardized One-Step open RT-qPCR protocol detect SARS-CoV-2 RNA clinical...