A5092554007- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- RNA modifications and cancer
- Nuclear Structure and Function
Goethe University Frankfurt
2023-2024
Splicing of pre-mRNAs critically contributes to gene regulation and proteome expansion in eukaryotes, but our understanding the recognition pairing splice sites during spliceosome assembly lacks detail. Here, we identify multidomain RNA-binding protein FUBP1 as a key splicing factor that binds hitherto unknown cis-regulatory motif. By collecting NMR, structural, vivo interaction data, demonstrate stabilizes U2AF2 SF1, components at 3' site, through multivalent binding interfaces located...
Nuclear RNA binding proteins (RBPs) are difficult to study because they often belong large protein families and form extensive networks of auto- crossregulation. They highly abundant many localize condensates with a slow turnover, requiring long depletion times or knockouts that cannot distinguish between direct indirect compensatory effects. Here, we developed system is optimized for the rapid degradation nuclear RBPs, called hGRAD. It comes as “one-fits-all” plasmid, integration into any...
Abstract Nuclear speckles (NS) and paraspeckles (PS) are adjacent condensates with distinct protein composition, serine-arginine-rich splicing factors (SRSFs) concentrated in NS. Surprisingly, we find that SRSF5 is present both. Combining super-resolution imaging, proximity proteomics iCLIP, show binds PS core proteins to the PS-scaffold RNA NEAT1 locates between spheres. Acute depletion results reduced differently packaged . Under stress, SRSF5’s association increases, without SRSF5,...