A5056706417- PI3K/AKT/mTOR signaling in cancer
- Autophagy in Disease and Therapy
- CRISPR and Genetic Engineering
- DNA Repair Mechanisms
- Bioactive Compounds and Antitumor Agents
- Organ Transplantation Techniques and Outcomes
- RNA Research and Splicing
- Enzyme function and inhibition
- Microtubule and mitosis dynamics
- Hormonal and reproductive studies
- Sperm and Testicular Function
- Airway Management and Intubation Techniques
- Inertial Sensor and Navigation
- Gene expression and cancer classification
- Rural development and sustainability
- Cancer-related Molecular Pathways
- Social Issues and Policies
- Amino Acid Enzymes and Metabolism
- Aging, Elder Care, and Social Issues
- Nuclear Structure and Function
- Cancer Genomics and Diagnostics
- Pulsars and Gravitational Waves Research
- Nitric Oxide and Endothelin Effects
- Cancer Mechanisms and Therapy
- Social Policy and Reform Studies
GW Pharmaceuticals (United Kingdom)
2009-2023
Johns Hopkins University Applied Physics Laboratory
2023
Johns Hopkins University
2017
AstraZeneca (United Kingdom)
2009-2012
Howard Hughes Medical Institute
1996
Rockefeller University
1996
Ludwig-Maximilians-Universität München
1987-1989
The mammalian target of rapamycin (mTOR) kinase forms two multiprotein complexes, mTORC1 and mTORC2, which regulate cell growth, survival, autophagy. Allosteric inhibitors mTORC1, such as rapamycin, have been extensively used to study tumor proliferation, autophagy but shown only limited clinical utility. Here, we describe AZD8055, a novel ATP-competitive inhibitor mTOR activity, with an IC50 0.8 nmol/L. AZD8055 showed excellent selectivity (approximately 1,000-fold) against all class I...
We have isolated a major protein constituent from highly enriched fraction of yeast nuclear pore complexes (NPCs). The gene encoding this protein, Nup188p, was cloned, sequenced, and found to be nonessential upon deletion. Nup188p cofractionates with NPCs gives an immunofluorescent staining pattern typical nucleoporins. Using immunoelectron microscopy, shown localize both the cytoplasmic nucleoplasmic faces NPC core. There, interacts integral membrane domain, Pom152p, another abundant...
Abstract The DNA damage response (DDR) comprises a range of mechanisms that ensure the integrity genome. As part DDR and in to specific damage, master kinases ATM, ATR DNA-PK are activated coordinated fashion inducing cell cycle arrest repair before resuming replication. Alternatively they may induce apoptosis if is incompatible with viability. Due their genetic instability, tumour cells be more reliant on so inhibiting components lead antitumour activity while minimizing toxicity normal...
ATPase-deficient foci in rat liver, considered to be clonal origin and possible precursors of hepatocellular carcinomas, were induced by injecting male Wistar AF/Han (200-220 g) rats with N-methyl-N-nitrosourea (MNU) (25 mg/kg) 16 or 22 h after partial hepatectomy feeding for 80 days a diet containing phenobarbital. The animals killed 90 the intralobular distribution preneoplastic was analysed quantitatively. locations 48 16-h group 22-h determined from serial sections blocks liver five...
Abstract Changes in the transformation sensitivity of hepatocytes during cell cycle were relation to clonogenicity investigated partially resected rat liver. The hourly rate G 1 ‐S transit was measured a control group by means tritium‐labelled thymidine (H‐TdR). At defined periods after partial hepatectomy animals injected with single dose N‐methyl‐N‐nitrosourea (MNU) (25mg/kg) and subsequently exposed phenobarbital (0.05% diet) for 80 days. ATPase‐deficient populations which arise clonal...
<div>Abstract<p>The mammalian target of rapamycin (mTOR) kinase forms two multiprotein complexes, mTORC1 and mTORC2, which regulate cell growth, survival, autophagy. Allosteric inhibitors mTORC1, such as rapamycin, have been extensively used to study tumor proliferation, autophagy but shown only limited clinical utility. Here, we describe AZD8055, a novel ATP-competitive inhibitor mTOR activity, with an IC<sub>50</sub> 0.8 nmol/L. AZD8055 showed excellent selectivity...
<div>Abstract<p>The mammalian target of rapamycin (mTOR) kinase forms two multiprotein complexes, mTORC1 and mTORC2, which regulate cell growth, survival, autophagy. Allosteric inhibitors mTORC1, such as rapamycin, have been extensively used to study tumor proliferation, autophagy but shown only limited clinical utility. Here, we describe AZD8055, a novel ATP-competitive inhibitor mTOR activity, with an IC<sub>50</sub> 0.8 nmol/L. AZD8055 showed excellent selectivity...
The increasing number of private and public actors interested in space-based missions has driven need for greater flexibility reliability regards to navigation. Autonomous navigation space will reduce reliance on ground-based systems high operational costs due crowded communication networks. Further, there is a clear autonomous solutions GPS-denied environments, as well deep-space regions which traditional GPS methods are infeasible. One promising approach achieving the dynamic landscape...