A5076316263- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Neural dynamics and brain function
- Advanced Fluorescence Microscopy Techniques
- Genetic Neurodegenerative Diseases
- Biochemical effects in animals
- Mitochondrial Function and Pathology
- Neurogenesis and neuroplasticity mechanisms
- Stress Responses and Cortisol
- Gene Regulatory Network Analysis
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell Image Analysis Techniques
- Receptor Mechanisms and Signaling
- Anesthesia and Neurotoxicity Research
- Neuroscience and Neural Engineering
- Chemical Reactions and Isotopes
- Neuroendocrine regulation and behavior
- Photoreceptor and optogenetics research
- DNA Repair Mechanisms
- Muscle Physiology and Disorders
SickKids Foundation
2024-2025
Hospital for Sick Children
2018-2025
University of Toronto
2018-2025
McMaster University
2016
Huntington's disease (HD) is an age-dependent neurodegenerative disease. DNA repair pathways have recently been implicated as the most predominant modifiers of age onset in HD patients. We report that endogenous huntingtin protein directly participates oxidative damage repair. Using novel chromobodies to detect human live cells, we show localization sites dependent on kinase activity ataxia telangiectasia mutated (ATM) protein. Super-resolution microscopy and biochemical assays revealed...
Abstract Miniaturized fluorescence microscopes for imaging calcium transients are a promising tool investigating the relationship between behavior and population‐level neuronal activity in rodents. However, commercially available miniature may be costly and, because they closed source, not easily modified based on particular experimental requirements. Here, we describe how to build use low‐cost compact head‐mounted endoscope (CHEndoscope) system vivo imaging. The CHEndoscope uses an...
The N17 domain of the huntingtin protein is post-translationally modified and master regulator intracellular localization. In Huntington's disease (HD), mutant hypo-phosphorylated at serines 13 16 within N17, increasing phosphorylation has been shown to be protective in HD mouse models. Thus, defined as a sub-target for potential therapeutic intervention. We have previously that cellular stress can affect nuclear entry phosphorylation. Here, we demonstrate localization specifically affected...
Memories of events are linked to the contexts in which they were encoded. This contextual linking ensures enhanced access those memories that most relevant context at hand, including specific associations previously learned context. principle, referred as encoding specificity, predicts context-specific neural states should bias retrieval particular over others, potentially allowing for disambiguation cues may have multiple or meanings. Using a context-odor paired associate learning paradigm...
In vivo 1-photon calcium imaging is an increasingly prevalent method in behavioural neuroscience. Numerous analysis pipelines have been developed to improve the reliability and scalability of pre-processing ROI extraction for these large datasets. Despite advancements methods, manual curation extracted spatial footprints traces neurons remains important quality control. Here, we propose additional semi-automated step sorting from putative using popular CNMF-E algorithm. We used automated...
Abstract Miniaturized fluorescence microscopes for imaging calcium transients are a promising tool investigating the relationship between behaviour and population-level neuronal activity in rodents. However, commercially available miniature may be costly, and, because they closed-source, not easily modified based on particular experimental requirements. Here, we describe how to build use low-cost c ompact h ead-mounted e ndoscope (CHEndoscope) system vivo imaging. The CHEndoscope uses an...