ADVERTISEMENT RETURN TO ISSUEPREVCommunicationNEXT2D Covalent Organic Frameworks with Alternating Triangular and Hexagonal PoresLuke A. Baldwin, Jonathan W. Crowe, Matthew D. Shannon, Christopher P. Jaroniec, Psaras L. McGrier*View Author Information Department of Chemistry & Biochemistry, The Ohio State University, Columbus, 43210, United States*P. McGrier. E-mail: [email protected]Cite this: Chem. Mater. 2015, 27, 18, 6169–6172Publication Date (Web):August 2015Publication History Received1...

Double electron resonance (DEER) is an attractive technique that utilized for gaining insight into protein structure and dynamics via nanometer-scale distance measurements. The most commonly used paramagnetic tag in these measurements a nitroxide spin label, R1. Here, we present the application of two types high-affinity Cu(2+) chelating tags, based on EDTA cyclen metal-binding motifs as alternative X-band DEER probes, using B1 immunoglobulin-binding domain G (GB1) model system. Both tags...

The interaction of positively charged N-terminal histone tails with nucleosomal DNA plays an important role in chromatin assembly and regulation, modulating their susceptibility to post-translational modifications recognition by chromatin-binding proteins. Here, we report residue-specific 15 N NMR relaxation rates for H4 reconstituted nucleosomes. These data indicate that are strongly dynamically disordered, albeit reduced conformational flexibility compared a free peptide the same sequence....

The majority of base pairs in double-stranded DNA exist the canonical Watson-Crick geometry. However, they can also adopt alternate Hoogsteen conformations various complexes with proteins and small molecules, which are key for biological function mechanism. While detection large assemblies poses considerable challenges traditional structural biology techniques, we show here that multidimensional dynamic nuclear polarization–enhanced solid-state NMR serve as a unique spectroscopic tool...

The fundamental repeat unit of chromatin, the nucleosome, consists approximately 147 base pairs double-stranded DNA and a histone protein octamer containing two copies each histones H2A, H2B, H3, H4. Each possesses dynamically disordered N-terminal tail domain, it is well-established that tails H3 H4 play key roles in chromatin compaction regulation. Here we investigate conformational ensemble interactions nucleosomes by means solution NMR measurements paramagnetic relaxation enhancements...

Chromatin is a supramolecular DNA-protein complex that compacts eukaryotic genomes and regulates their accessibility functions. Dynamically disordered histone H3 N-terminal tails are among key chromatin regulatory components. Here, we used high-resolution-magic-angle-spinning NMR measurements of backbone amide 15N spin relaxation rates to investigate, with residue-specific detail, the dynamics interactions in recombinant 13C,15N-enriched nucleosome arrays containing 15, 30, or 60 bp linker...

Abstract Iranian students amassed one of the most impressive movements 1960s. Through diligent and efficient transnational organizing in United States Western Europe, oppositionist wedded their rejection US-supported regime Shah Mohammad Reza Pahlavi with a broader, more inclusive internationalism that thrived late This essay situates student movement abroad -- both its challenges to Washington-Tehran alliance within context US international history. Rather than establish maintain systematic...

Microsecond to millisecond timescale backbone dynamics of the amyloid core residues in Y145Stop human prion protein (PrP) fibrils were investigated by using

We demonstrate rapid quantitative measurements of site-resolved paramagnetic relaxation enhancements (PREs), which are a source valuable structural restraints corresponding to electron–nucleus distances in the ∼10–20 Å regime, solid-state nuclear magnetic resonance (NMR) spectra proteins containing covalent Cu2+-binding tags. Specifically, using protein GB1 K28C-EDTA-Cu2+ mutant as model, we show determination backbone amide 15N longitudinal and 1H transverse PREs within few hours experiment...

Abstract The interaction of positively charged N‐terminal histone tails with nucleosomal DNA plays an important role in chromatin assembly and regulation, modulating their susceptibility to post‐translational modifications recognition by chromatin‐binding proteins. Here, we report residue‐specific 15 N NMR relaxation rates for H4 reconstituted nucleosomes. These data indicate that are strongly dynamically disordered, albeit reduced conformational flexibility compared a free peptide the same...

This work outlines a full-semester undergraduate biochemistry laboratory module that is mostly synchronous with the lecture. The pedagogical motivation and design of these experiments are to create experience engages students, prepares students for research, helps learn challenging biochemical topics. experiment begins bioinformatics study four proteins used: Horseradish peroxidase (HRP), human serum albumin (HSA), lysozyme, myoglobin. Initially, HSA HRP purified using anion-exchange...

Journal Article David S. Painter and Gregory Brew. The Struggle for Iran: Oil, Autocracy, the Cold War, 1951–1954. Get access Chapel Hill, NC: University of North Carolina Press, 2022. Pp. 324. Paper $32.95. Matthew Shannon Emory Henry University, US Email: [email protected] Search other works by this author on: Oxford Academic Google Scholar American Historical Review, Volume 129, Issue 4, December 2024, Pages 1778–1779, https://doi.org/10.1093/ahr/rhae374 Published: 05 2024

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The covert cold war that the Central Intelligence Agency (CIA) waged for hearts and minds of international students came to an abrupt end with Ramparts exposes early 1967. i...

Nathan Citino uses the theme of “modernization” to explain U.S.-Middle East relations between 1945 and 1967. He demonstrates how, after Second World War, modernization became manifest in “not only economic development policies, but also ideas elites used social change” (1). Those elites, Arab American, considered quantitative qualitative aspects within legacy Ottoman reforms, prospect secular nation-building, politics Cold Palestinian question. A veteran researcher American histories,...

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The advent of “modern” education in Iran and its acceptance by political cultural elites dates to the Qajar era. But elitist nature state reforms prevented modern from spreading throughout society until Pahlavi Especially during reign Mohammad Reza Shah Pahlavi, reached most segments population, including religious families middle classes. This research is based on Persian-language documents informed English-language historiography. article finds that Islamic Education Society (Jāmeʿeh-ye...