In order to understand the pulmonary toxicity of ultrafine titanium dioxide (UF-TiO2) particles, various biochemical and chemical parameters were assayed in rat alveolar macrophages (AMs) cell-free lavage fluid. Single intratracheal exposure UF-TiO2 (2 mg per rat) caused cytotoxicity AMs. An increase population AMs could be observed, followed by increased activities lactate dehydrogenase acid phosphatase addition, showed an adaptive response because glutathione peroxidase, reductase,...

The increased inhaled application of titanium dioxide nanoparticles (TiO(2) NPs) increases the potential pulmonary health risks. present investigations were carried out to study TiO(2) NPs-induced apoptosis, oxidative stress and genotoxicity in human lung cancer cell line, A549, a widely used system for toxicity studies. Tetrazolium bromide salt lactate dehydrogenase release assays cytotoxicity. studies using cytokinesis block micronucleus assay. Apoptosis was confirmed by formation...

Surface-treated titanium dioxide (TiO(2)) particles coated with vanadium pentoxide (V(2)O(5)) are used industrially for selective catalytic reactions such as the removal of nitrous oxide from exhaust gases combustion power plants (SCR process) and in biomaterials increasing strength implants. In present study, untreated ultrafine TiO(2) (anatase, diameter: 30-50 nm) (V(2)O(5))-treated anatase were tested their cyto- genotoxic effects V79 cells (hamster lung fibroblasts). Cytotoxic assessed...

Coat color variations in cattle are known to be influenced by the melanocortin 1 receptor (MC1R) and tyrosine kinase (KIT) genes. The presence of coat abnormalities, such as white spots albinism, Bali was focus this study. This study aimed identify single nucleotide polymorphisms (SNPs) coding region MC1R exons 2 3 KIT associated with abnormalities cattle. included analysis 48 cattle, including 20 individuals standard color, 15 spots, 13 albinism. Total DNA extracted using a Extraction Kit,...

Exposure to asbestos minerals has been associated with a wide variety of adverse health effects including lung cancer, pleural mesothelioma, and cancer other organs. It was shown previously that samples collected from local factory enhanced sister chromatid exchanges (SCEs) chromosomal aberrations in vitro using human lymphocytes. In the present study, 22 workers same 12 controls were further investigated. Controls matched for age, sex, socioeconomic state. The peripheral blood lymphocytes...

BackgroundType 2 diabetes mellitus (T2DM) is a global major health problem resulting from interaction of environmental and genetic factors, examples the latter being KCNJ11 (coding for part ATP-sensitive potassium channel) SDF-1β chemokine CXCL12). Our case-control study was conducted to assess whether recessive, dominant or additive genotype model associations (E23K, rs5219) (G801A, rs1801157) were more strongly linked type diabetes.Subjects & MethodsGenetic polymorphism analysis performed...

Lysis of erythrocytes in vitro leading to release hemoglobin has often been used as a convenient test system for predictive toxicological evaluation noxious particulates encountered pollutants occupational and environmental atmospheres. However, spite several studies with silica silicate dusts, the exact biochemical mechanism membrane damage is not clear. Therefore, detailed were conducted. Screening large number fugitive dust samples indicated that dissolution under assay conditions...

Silicic acid and silicate dusts (slate dust chrysotile asbestos) cause hemolysis of erythrocytes in vitro. The peroxidation polyunsaturated fatty acids (PUFA) erythrocyte membrane lipids is also enhanced by incubating the with silicic Hemolysis elicited inhibited significantly polyvinyl-pyrrolidone dipalmitoyl lecithin (DPL). These agents, however, have no effect on induced lipids. On other hand, lipids, dusts, almost completely adding superoxide dismutase catalase to incubation system,...

The contents of collagen, hexosamine, phospholipids, and cholesterol the activities acid alkaline phosphatases, glutamic oxalo-acetate transaminase, pyruvate transminase, aldolase, hexokinase, lactic dehydrogenase were determined in lungs rats 150 days after intratracheal injection amosite, anthophyllite, chyrsotile. Anthophyllite did not cause any significant change, while amosite chrysotile caused increases collagen mucopolysaccharides. Lactic phosphatase increased by all dusts, othe...

Several observations, including studies from this laboratory, demonstrate that asbestos generates free radicals in the biological system may play a role manifestation of asbestos-related cytotoxicity and carcinogenicity. It has also been demonstrated iron associated with plays an important asbestos-mediated generation reactive oxygen species. Exposure to leads degradation heme proteins such as cytochrome P450-releasing cytosol. Our simulation experiments presence show asbestos-released...

This article reports the effect of coexposure to Indian chrysotile asbestos (5 mg/rat) and kerosene soot on pulmonary phase I II drug-metabolizing enzymes 1, 4, 8, 16, 30, 90, 150 days after a single intratracheal inoculation. Exposure resulted in significant induction microsomal cytochrome P450 activity dependent monooxygenase, benzo(a)pyrene (B[a]P) hydroxylase, epoxide hydrase at all time intervals. On other hand, cytosolic glutathione S-transferase (GST) was induced 30 exposure, followed...

Abstract Asbestos is known to cause lung diseases in occupationally exposed workers. These properties have restricted its use. Industries been exploring the possibility of other mineral fibres replace asbestos. In this direction, wollastonite has gained great attention owing high thermal resistance. present paper, toxicity three samples Indian was compared that chrysotile. Dust suspensions were added red blood cell obtain a final dust concentration 1.0–5.0 mg ml −1 system. The varieties...

The dust generated during weaving (carpet dust) tibbati, knotted and tuffted carpets in carpet industry was studied for its toxicity vitro vivo. Carpet (0.5, 1.0, 2.5 5.0 mg/1£10 6 cells) caused cytotoxicity rat alveolar macrophages (AM) a concentration-dependent manner. cytotoxic, inflammatory oxidative responses were observed bronchoalveolar lavage fluid (BALF) of rats at 1, 4, 8 16 days after exposure. Rats intratracheally exposed 5 mg/rat individually to all three types dust. All dusts...

As part of a detailed experimental study the pathogenicity disease slate dust workers, early biochemical changes in rat lung from 1 to 90 days after intratracheal inoculation particle size below 5 micron were investigated. A severalfold increase free cell population (initially macrophages) was elicited by dust. The activity acid phosphatase tended along with break lysosomal latency increasing exposure period. However, actual release enzyme into acellular fraction low. phospholipid content...