A5076108824- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Cancer Research and Treatments
- CAR-T cell therapy research
- Animal Virus Infections Studies
- Advanced biosensing and bioanalysis techniques
Chimie et Interdisciplinarité, Synthèse, Analyse, Modélisation
2023-2025
Inserm
2024-2025
Nantes Université
2023-2025
Centre National de la Recherche Scientifique
2023-2025
Gene Therapy Laboratory
2023-2024
Translational Research in Gene Therapy
2024
Centre Hospitalier Universitaire de Nantes
2024
Abstract Methods for direct covalent ligation of microorganism surfaces remain poorly reported, and mostly based on metabolic engineering bacteria cells functionalization. While effective, a faster method avoiding the bio-incorporation step would be highly complementary. Here, we used N -methylluminol (NML), fully tyrosine-selective protein anchoring group after one-electron oxidation, to label surface viruses, living cells. The functionalization was performed electrochemically in situ by...
Decades of biological and clinical research have led to important advances in recombinant adeno-associated viruses rAAV-based gene therapy therapy. However, several challenges must be overcome fully exploit the potential rAAV vectors. Innovative approaches modify viral genome capsid elements been used issues such as unwanted immune responses off-targeting. While often successful, genetic modification capsids can drastically reduce vector yield fails produce vectors with properties that...
We report the chemical conjugation of a recombinant Adeno Associated Virus (rAAV) capsid with various functionalities, including proteins, using bioorthogonal strategy. rAAVs were azido-coated or dibenzylcyclooctyne (DBCO)-coated by chemically modifying lysine tyrosine residues. Lysine residues modified phenyl isothiocyanate anchor, and either an aryl diazonium salt N-methyl luminol derivative. demonstrate anchor-dependent labeling levels, as observed biochemical assays mass spectrometry....
We report the chemical conjugation of recombinant Adeno Associated Virus (rAAV) capsid with various functionalities, including proteins, using a bioorthogonal strategy. rAAVs were azido-coated or DBCO-coated by chemically modifying lysine tyrosine residues. Lysine residues modified phenyl isothiocyanate anchor, and either an aryldiazonium salt N-methyl luminol derivative. demonstrate anchor- dependent labelling levels, as observed biochemical assays mass spectrometry. Strain-promoted...
Decades of biological and clinical research have led to important advances in recombinant adeno-associated viruses rAAV-based gene therapy therapy. However, several challenges must be overcome fully exploit the potential rAAV vectors. Innovative approaches modify viral genome capsid elements been used issues such as unwanted immune responses off-targeting. While often successful, genetic modification capsids can drastically reduce vector yield fails produce vectors with properties that...
Abstract This study investigates novel approaches to improve targeted gene delivery the liver, a crucial organ for metabolic processes that faces vulnerabilities from various pathologies. Adeno-associated virus (AAV)-based therapy has emerged as promising approach liver targeting, with numerous investigational avenues. However, administration of high doses AAV vectors present safety concerns, often requiring use corticosteroids and immunosuppression mitigate immune adverse events. To address...
The remodeling of microorganism surfaces with biomolecules is a powerful tool to study the role membrane receptors in chemical biology and develop drug delivery systems gene therapy using viral vectors cell-based therapies. Methods for direct covalent ligation these remain poorly reported, mostly based on metabolic engineering bacteria cells functionalization. In latter case, tagged precursor must first be enzymatically metabolized delivered outer cell become available chemo-selective...