A5040256984- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Microbial infections and disease research
- Bacterial Identification and Susceptibility Testing
- Antibiotic Resistance in Bacteria
- Infective Endocarditis Diagnosis and Management
- Vibrio bacteria research studies
- Ocular Infections and Treatments
- Plant Virus Research Studies
- Streptococcal Infections and Treatments
- Escherichia coli research studies
- Genomics and Phylogenetic Studies
- Nematode management and characterization studies
- Microbial Metabolism and Applications
- Soybean genetics and cultivation
- Cystic Fibrosis Research Advances
- Pneumonia and Respiratory Infections
- Legume Nitrogen Fixing Symbiosis
- Clostridium difficile and Clostridium perfringens research
- Respiratory viral infections research
University of Pittsburgh
2020-2024
Icahn School of Medicine at Mount Sinai
2023
University of Minnesota
1998
Patients with hematological malignancies or undergoing hematopoietic stem cell transplantation are vulnerable to colonization and infection multidrug-resistant organisms, including vancomycin-resistant Enterococcus faecium (VREfm). Over a 10-y period, we collected sequenced the genomes of 110 VREfm isolates from gastrointestinal blood cultures 24 pediatric patients chemotherapy for malignancy at St. Jude Children's Research Hospital. We used patient-specific reference identify variants that...
Abstract Background There is increased interest in bacteriophage (phage) therapy to treat infections caused by antibiotic‐resistant bacteria. A lung transplant recipient with cystic fibrosis and Burkholderia multivorans infection was treated inhaled phage for 7 days before she died. Methods Phages were given via nebulization through the mechanical ventilation circuit. Remnant respiratory specimens serum collected. We quantified bacterial deoxyribonucleic acid (DNA) using quantitative...
Pseudomonas aeruginosa infections can be difficult to treat and new therapeutics are needed. Bacteriophage therapy is a promising alternative traditional antibiotics, but large numbers of isolated characterized phages lacking. We collected 23 diverse P. isolates from people with cystic fibrosis (CF) clinical infections, used them screen isolate over dozen aeruginosa-targeting hospital wastewater. Phages were genome sequencing, comparative genomics, lytic activity screening against all...
Abstract Objectives To investigate the genomic diversity and β-lactam susceptibilities of Enterococcus faecalis collected from patients with infective endocarditis (IE). Methods We 60 contemporary E. isolates definite or probable IE cases identified between 2018 2021 at University Pittsburgh Medical Center. used whole-genome sequencing to study bacterial employed antibiotic checkerboard assays a one-compartment pharmacokinetic–pharmacodynamic (PK/PD) model susceptibility ampicillin...
Enterococcus faecalis are hospital-associated opportunistic pathogens and also causative agents of post-operative endophthalmitis. Patients with enterococcal endophthalmitis often have poor visual outcomes, despite appropriate antibiotic therapy. Here we investigated the genomic phenotypic characteristics E. isolates collected from 13 patients treated at University Pittsburgh Medical Center Eye over 19 years. Comparative analysis indicated that were infected belonging to diverse multi-locus...
Burkholderia spp. are often resistant to antibiotics, and infections with these organisms difficult treat. A potential alternative treatment for is bacteriophage (phage) therapy; however, it can be locate phages that target bacteria. Prophages incorporated into the bacterial genome have been identified within may represent a source of useful therapy. Here, we investigate whether prophages clinical isolates kill conspecific heterospecific isolates. Thirty-two were induced prophage release,...
Abstract Burkholderia spp. are often resistant to antibiotics, and infections with these organisms difficult treat. A potential alternative treatment for is bacteriophage (phage) therapy; however, it can be locate phages that target bacteria. Prophages incorporated into the bacterial genome have been identified within may represent a source of useful therapy. Here we investigate whether prophages clinical isolates kill conspecific heterospecific isolates. Thirty-two were induced prophage...
Escherichia coli multi-locus sequence type ST131 is a globally distributed pandemic lineage that causes multidrug-resistant extra-intestinal infections. E. frequently produce extended-spectrum β-lactamases (ESBLs), which confer resistance to many β-lactam antibiotics and make infections difficult treat. We sequenced the genomes of 154 ESBL-producing clinical isolates belonging from patients at University Pittsburgh Medical Center (UPMC) between 2004 2018. Isolates belonged well described...
Vancomycin-resistant enterococcal (VRE) bacteremia is associated with higher mortality rates and longer hospitalizations than vancomycin-sensitive (VSE) bacteremia. A 67-year-old man a right psoas abscess pacemaker-associated tricuspid valve endocarditis in September 2020 grew VSE Enterococcus faecium from blood cultures that cleared after administration of intravenous vancomycin gentamicin. Subsequently, he underwent repair, pacemaker removal, partial lead extraction. Valve postoperative...
Abstract Escherichia coli belonging to sequence type ST131 constitute a globally distributed pandemic lineage that causes multidrug-resistant extra-intestinal infections. E. frequently produce extended-spectrum β-lactamases (ESBLs), which confer resistance many β-lactam antibiotics and make infections difficult treat. We sequenced the genomes of 154 ESBL-producing clinical isolates from patients at University Pittsburgh Medical Center (UPMC) between 2004 2018. Isolates belonged well...
ABSTRACT Enterococcus faecalis are hospital-associated opportunistic pathogens and also causative agents of post-operative endophthalmitis. Patients with enterococcal endophthalmitis often have poor visual outcomes, despite appropriate antibiotic therapy. Here we investigated the genomic phenotypic characteristics E. isolates collected from 13 patients treated at University Pittsburgh Medical Center Eye over 19 years. Comparative analysis indicated that were infected diverse multi-locus...
Abstract Enterococcus faecalis is a leading cause of infective endocarditis (IE), especially among older patients with comorbidities. Here we investigated the genomic diversity and antimicrobial susceptibility 33 contemporary E. isolates from definite or probable IE cases at University Pittsburgh Medical Center (UPMC) between 2018 2020. Isolates belonging to two multi-locus sequence types (STs), ST6 ST179, were isolated nearly 40% patients. Both these dominant STs carried known beta-lactam...
Abstract Pseudomonas aeruginosa infections can be difficult to treat and new therapeutic approaches are needed. Bacteriophage therapy is a promising alternative traditional antibiotics, but large numbers of isolated characterized phages lacking. We collected 23 genetically phenotypically diverse P. isolates from people with cystic fibrosis (CF) clinical infections, their genetic, phenotypic, prophage diversity. then used these screen isolate 14 -targeting hospital wastewater. Phages were...