A5062376285- Phytoplasmas and Hemiptera pathogens
- Plant Pathogenic Bacteria Studies
- Insect symbiosis and bacterial influences
- Genomics and Phylogenetic Studies
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
- Cocoa and Sweet Potato Agronomy
- Escherichia coli research studies
- Studies on Chitinases and Chitosanases
- Plant Virus Research Studies
- Bacterial Identification and Susceptibility Testing
- Microbial Community Ecology and Physiology
- Antimicrobial Resistance in Staphylococcus
- Plant tissue culture and regeneration
- Plant Taxonomy and Phylogenetics
- Mycorrhizal Fungi and Plant Interactions
- Seed Germination and Physiology
- Infections and bacterial resistance
- Plant and Fungal Interactions Research
- Yersinia bacterium, plague, ectoparasites research
- RNA modifications and cancer
- Turfgrass Adaptation and Management
- Plant Parasitism and Resistance
- Plant-Microbe Interactions and Immunity
- Entomopathogenic Microorganisms in Pest Control
University of Pittsburgh
2021-2024
Institute of Plant and Microbial Biology, Academia Sinica
2015-2023
National Taiwan University
2023
Certain obligate parasites induce complex and substantial phenotypic changes in their hosts ways that favor transmission to other trophic levels. However, the mechanisms underlying these remain largely unknown. Here we demonstrate how SAP05 protein effectors from insect-vectored plant pathogenic phytoplasmas take control of several developmental processes. These simultaneously prolong host lifespan witches' broom-like proliferations leaf sterile shoots, organs colonized by vectors. acts...
Pseudomonas fluorescens PCL1751 is a rod-shaped Gram-negative bacterium isolated from the rhizosphere of greenhouse-grown tomato plant in Uzbekistan. It controls several root diseases caused by Fusarium fungi through mechanism competition for nutrients and niches (CNN). This does not rely on production antibiotics, so it avoids concerns resistance development environmentally safe. Additionally, this promotes growth alleviating salt stress its host. To investigate genetic mechanisms that may...
Most hospitals use traditional infection prevention (IP) methods for outbreak detection. We developed the Enhanced Detection System Healthcare-Associated Transmission (EDS-HAT), which combines whole-genome sequencing (WGS) surveillance and machine learning (ML) of electronic health record (EHR) to identify undetected outbreaks responsible transmission routes, respectively.We performed WGS healthcare-associated bacterial pathogens from November 2016 2018. EHR ML was used routes WGS-detected...
Abstract Background There is increased interest in bacteriophage (phage) therapy to treat infections caused by antibiotic‐resistant bacteria. A lung transplant recipient with cystic fibrosis and Burkholderia multivorans infection was treated inhaled phage for 7 days before she died. Methods Phages were given via nebulization through the mechanical ventilation circuit. Remnant respiratory specimens serum collected. We quantified bacterial deoxyribonucleic acid (DNA) using quantitative...
Bacteria of the genus 'Candidatus Phytoplasma' are uncultivated intracellular plant pathogens transmitted by phloem-feeding insects. They have small genomes lacking genes for essential metabolites, which they acquire from either or insect hosts. Nonetheless, some phytoplasmas, such as 'Ca. P. solani', broad host range and several polyphagous species. To understand better how these obligate symbionts can colonize a wide hosts, genome solani' strain SA-1 was sequenced infected periwinkle via...
Phytoplasmas are plant-pathogenic bacteria that impact agriculture worldwide. The commonly adopted classification system for phytoplasmas is based on the restriction fragment length polymorphism (RFLP) analysis of their 16S rRNA genes. With increased availability phytoplasma genome sequences, can now be refined. This work examined 11 strains in 16SrI group within genus 'Candidatus Phytoplasma' and investigated possible species boundaries. We confirmed RFLP method problematic due to...
Abstract Background Many named species as defined in current bacterial taxonomy correspond to complexes. Uncertainties regarding the organization of their genetic diversity challenge research efforts. We utilized Agrobacterium tumefaciens complex (a.k.a. biovar 1), a taxon known for its phytopathogenicity and applications transformation, study system devised strategies investigating genome evolution Results 35 assemblies, including 14 newly generated ones, achieve phylogenetically balanced...
Hospitalized patients are at increased risk of becoming infected with antibiotic-resistant organisms. We used whole-genome sequencing to survey and compare over 3,000 clinical bacterial isolates collected from hospitalized a large medical center 2-year period.
Phytoplasmas are insect-transmitted plant pathogens that cause substantial losses in agriculture. In addition to economic impact, phytoplasmas induce distinct disease symptoms infected plants, thus attracting attention for research on molecular plant-microbe interactions and developmental processes. Due the difficulty of establishing an axenic culture these bacteria, culture-independent genome characterization is a crucial tool phytoplasma research. However, genomes have strong nucleotide...
Common bermudagrass (Cynodon dactylon (L.) Pers.) belongs to the subfamily Chloridoideae of Poaceae family, one most important plant families ecologically and economically. This grass has a long connection with human culture but its systematics is relatively understudied. In this study, we sequenced investigated chloroplast genome common bermudagrass, which 134,297 bp in length two single copy regions (LSC: 79,732 bp; SSC: 12,521 bp) pair inverted repeat (IR) (21,022 bp). The annotation...
Type VI secretion system (T6SS) is a contractile nanoweapon employed by many Proteobacteria to deliver effectors kill or inhibit their competitors. One T6SS gene, vgrG, encodes spike protein for effector translocation and often present as multiple copies in bacterial genomes. Our phylogenomic analyses sampled 48 genomes across diverse lineages found ∼70% of them encode VgrGs, yet only four have nearly identical paralogs. Among these four, Agrobacterium tumefaciens 1D1609 has the highest vgrG...
Abstract Background Salmonella infection poses significant public health threat globally, especially in resource-limited countries. Emergence and spread of antibiotic resistant strains to fluoroquinolones have led treatment failures increased mortality infection. However, there is dearth information regarding mechanisms resistance Ghana. This study therefore sought identify chromosomal mutations plasmid-mediated as possible fluoroquinolone from clinical isolates Methods was a retrospective...
The type VI secretion system (T6SS) is used by gram-negative bacteria to translocate effectors that can antagonize other bacterial cells. Models predict the variation in collections of effector and cognate immunity genes determine competitiveness affect dynamics populations communities bacteria. However, outcomes competition cannot be entirely explained compatibility effector-immunity (EI) pairs. Here, we characterized diversity T6SS loci plant-pathogenic Agrobacterium tumefaciens showed...
Agrobacterium tumefaciens is a phytopathogenic bacterium that causes crown gall disease. The strain Ach5 was isolated from yarrow (Achillea ptarmica L.) and the wild-type progenitor of other derived strains widely used for plant transformation. Here, we report complete genome sequence this bacterium.
The bacterial genus Xylella contains plant pathogens that are major threats to agriculture in America and Europe. Although extensive research was conducted characterize different subspecies of fastidiosa ( Xf ), comparative analysis at above-species levels lacking due the unavailability appropriate data sets. Recently, a bacterium causes pear leaf scorch (PLS) Taiwan described as second species (i.e., taiwanensis ; Xt ). In this work, we report complete genome sequence type strain PLS229 T ....
The bacterial genus "Candidatus Phytoplasma" contains a group of insect-transmitted plant pathogens in the class Mollicutes. Here, we report draft genome assembly and annotation strain NCHU2014, which belongs to 16SrII-A subgroup within this is associated with purple coneflower witches' broom disease Taiwan.
"Candidatus Sulcia muelleri" is a symbiont of sap-feeding insects in the suborder Auchenorrhyncha. The strain "Ca. ML associated with maize leafhopper (Dalbulus maidis), collected Brazil, which disease vector that affects corn production. Here, we report complete genome sequence this bacterium.
The periwinkle leaf yellowing (PLY) disease was first reported in Taiwan 2005. This caused by an uncultivated bacterium the genus "Candidatus phytoplasma." In subsequent years, this linked to other plant diseases and losses agriculture. For genomic investigation of its relatives, we conducted whole genome sequencing a PLY phytoplasma from infected collected Taoyuan. de novo assembly produced eight contigs with total length 824,596 bp. annotation contains 775 protein-coding genes, 63...
The complete genome sequence of "Candidatus Phytoplasma asteris" RP166, which consists one 829,546-bp circular chromosome, is presented in this work. This bacterium associated with rapeseed phyllody disease Poland and belongs to the 16SrI-B (i.e., aster yellows) group.
Agrobacterium tumefaciens is important in biotechnology due to its ability transform eukaryotic cells. Although the molecular mechanisms have been studied extensively, previous studies were focused on model strain C58. Consequently, nearly all of commonly used strains for application derived from C58 and share similar host ranges. To overcome this limitation, better understanding natural genetic variation could provide valuable insights. In study, we conducted comparative analysis between...
Escherichia coli multi-locus sequence type ST131 is a globally distributed pandemic lineage that causes multidrug-resistant extra-intestinal infections. E. frequently produce extended-spectrum β-lactamases (ESBLs), which confer resistance to many β-lactam antibiotics and make infections difficult treat. We sequenced the genomes of 154 ESBL-producing clinical isolates belonging from patients at University Pittsburgh Medical Center (UPMC) between 2004 2018. Isolates belonged well described...
1D1609 is a highly virulent strain isolated from crown gall tumor of alfalfa (