A5101030091- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Endoplasmic Reticulum Stress and Disease
- Ion channel regulation and function
- Neuropeptides and Animal Physiology
- Heat shock proteins research
- Pain Mechanisms and Treatments
- Fungal and yeast genetics research
- Neurotransmitter Receptor Influence on Behavior
- Nerve injury and regeneration
- Redox biology and oxidative stress
- Genetic and Environmental Crop Studies
- Pancreatic function and diabetes
- RNA regulation and disease
- Satellite Communication Systems
- Ferroelectric and Piezoelectric Materials
- Agricultural pest management studies
- Adenosine and Purinergic Signaling
- Metallurgical and Alloy Processes
- Galectins and Cancer Biology
- Grouting, Rheology, and Soil Mechanics
- Software-Defined Networks and 5G
- Learning Styles and Cognitive Differences
- Microwave Dielectric Ceramics Synthesis
- Cassava research and cyanide
Xi'an Jiaotong University
2020-2024
Binzhou University
2024
Binzhou Medical University
2024
Sun Yat-sen University
2024
Chongqing Academy of Agricultural Sciences
2023
Hunan University
2022-2023
Wake Forest University
2000-2020
Karolinska Institutet
2001-2005
Baylor College of Medicine
2004-2005
Nobel Foundation
2004-2005
Recent reports have identified an endogenous peptide ligand for the opioid receptor-like (ORL1) receptor. In present study, ORL1 peptide-stimulated [35S]GTPγS binding was assessed in rat cortical membranes and brain sections to localize receptor-activated G-proteins. membrane assays, with 20 γM GDP, stimulated by approximately twofold ED50 value of nM. peptide- unaffected or other G-protein-coupled receptor antagonists. sections, regions including cortex, amygdala, hypothalamus, thalamus...
Abstract Although biochemical and physiological evidence suggests a strong interaction between striatal CB 1 cannabinoid (CB R) D 2 dopamine (D receptors, the mechanisms are poorly understood. We targeted medium spiny neurons of indirect pathway using shRNA to knockdown either R or R. Chronic reduction in receptor resulted deficits gene protein expression for alternative concomitantly increased interacting 1a (CRIP1a), suggesting novel role CRIP1a dopaminergic systems. Both reduced...
SHP1 has been documented as a tumor suppressor and it was thought to play an antagonistic role in the pathogenesis of Helicobacter pylori infection. In this study, exact mechanism action studied.
In vitro, protein disulfide isomerase (Pdi1p) introduces disulfides into proteins (oxidase activity) and provides quality control by catalyzing the rearrangement of incorrect (isomerase activity). Protein (PDI) is an essential in Saccharomyces cerevisiae, but contributions catalytic activities PDI to oxidative folding endoplasmic reticulum (ER) are unclear. Using variants Pdi1p with impaired oxidase or activity, we show that isomerase-deficient mutants support wild-type growth even a strain...
Glutaredoxin (Grx) and protein-disulfide isomerase (PDI) are members of the thioredoxin superfamily thiol/disulfide exchange catalysts. Thermodynamically, rat PDI is a 600-fold better oxidizing agent than Grx1 from Escherichia coli. Despite that, surprisingly good protein oxidase. It catalyzes disulfide formation in redox buffer with an initial velocity that 30-fold faster PDI. Catalysis peptide oxidation by individual catalytic domains Grx1-PDI chimera show differences active site chemistry...
Protein disulfide isomerase (PDI) is an essential protein folding assistant of the eukaryotic endoplasmic reticulum that catalyzes both formation disulfides during (oxidase activity) and isomerization may form incorrectly (isomerase activity). Catalysis thiol-disulfide exchange by PDI required for cell viability in Saccharomyces cerevisiae, but there has been some uncertainty as to whether role oxidase or isomerase. We have studied ability constructs with high activity very low complement...
Abstract 2‐Amino‐4,5,6,7‐tetrahydrobenzo(β)thiophen‐3‐yl 4‐chlorophenylmethanone (T62) is a member of group allosteric modulators adenosine A 1 receptors tested in animal models neuropathic pain to increase the efficacy adenosine. To determine its mechanisms at level receptor‐G‐protein activation, present studies examined effect T62 on ‐stimulated [ 35 S]guanosine‐5′‐O‐(γ‐thio)‐triphosphate ([ S]GTPγS) binding brain membranes, and by S]GTPγS autoradiography using agonist,...
Cholinesterase inhibitors which reach the central nervous system produce pain relief but are poorly tolerated because of gastrointestinal side effects. Here, authors tested whether donepezil, a penetrant cholinesterase inhibitor with low incidence effects, would relieve hypersensitivity in an animal model neuropathic pain.Male rats were anesthetized, and L5 L6 spinal nerves ligated unilaterally. Hypersensitivity was measured by withdrawal threshold to von Frey filament application hind paw...
Background Neuropathic pain alters opioid self-administration in rats. The brain regions altered the presence of neuropathic mediating these differences have not been identified, but likely involve ascending pathways interacting with limbic system. amygdala is a region that integrates noxious stimulation activity. Methods μ-Opioid receptors were blocked using irreversible antagonist, β-funaltrexamine, and antiallodynic reinforcing effects heroin determined spinal nerve-ligated In addition,...
Protein-disulfide isomerase (PDI) has five domains: a, b, b', a' and c, all of which except c have a thioredoxin fold. A single catalytic domain (a or a') is effective in catalyzing oxidation reduced protein but not isomerization disulfides (Darby, N. J., Creighton, T. E. (1995) Biochemistry 34, 11725-11735). To examine the structural basis for this oxidase activity PDI, shuffled mutants were generated using method that should be generally applicable to multidomain proteins. Domains along...
Pro-C creativity refers to professional creativity, which plays a key role in designing novel and practical works for designers work. In order verify the significance of critical thinking explore how improve through training, we carried out two studies. Study 1, recruited 58 using longitudinal research approach employed California Critical Thinking Skills Test (CCTST) Disposition Inventory (CCTDI) measure designers' thinking. Designers were tasked with completing assignments related level A,...
High-performance relaxor ferroelectric single crystals, such as Pb(Mg1/3Nb2/3)O3–PbTiO3 (PMN–PT), play a crucial role in the applications of medical ultrasonic imaging due to their high piezoelectric properties. In this study, we successfully grew 2-in. Eu-doped PMN–PT crystal whose composition variation and electrical properties were systematically investigated. It was found that PT content boule increased gradually along growth direction, while change Eu3+ opposite. The property results...
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Dynamic compaction is a cost-effective foundation treatment method which can improve the bearing capacity of foundation, reduce compressibility, eliminate collapsibility and ameliorate seismicity. With development along depth breadth, energy level dynamic also improves constantly. From 8000kN.m in 1991 to 18000kN.m 2008, has increased 10000kN.m 18 years. This project first experimental research using 20000 kN.m high process China. The shallow plate loading test, penetration laboratory soil...
Metabotropic glutamate (mGlu) receptors are regulators of release and targets for development therapies hyperactive glutamatergic signaling. However, the effects long-term stimulation mGlu on cellular signaling in brain have not been described. This study investigated 2-day 14-day osmotic mini-pump administration mGlu2,3 agonist LY379268 (3.0 mg kg