Dadih samples from two different origins (Kamang and Gadut in West Sumatra) manufactured with methods (back-slopping or spontaneous fermentation) were evaluated for the diversity of lactic acid bacteria (LAB).

Jembrana Disease is an infectious disease in Balinese cattle, which characterized by fever and inflammation of the oral mucous membranes (stomatitis, enlarged lymph nodes preskapularis, prefemolaric, parotid, sometimes accompanied blood sweating. This reaches a mortality rate no more than 20% caused Virus (JDV). According to data from Directorate Animal Health, Jembraa cases continue increase year year, with as many 398 2016 increased 4,797 2018. The presence virus can be detected through...

In response to environmental challenges and the critical imperative for sustainable progress, rural regions including agriculture practices are important in ecological robustness. It can be achieved through a synergistic relationship between development sustainability biological sciences. Concurrently, research stewardship approaches underway amass data on robusta coffee, Coffea canephora, species. collaboration with Lampung Disease Investigation Centre local coffee farmers Pesawaran,...

Lumpy Skin Disaese, LSD, was first discovered in Indonesia Riau Province February 2022. Although LSD is not a zoonotic disease, its infection can affect the production and reproductive performance of cattle, resulting various impacts including reduced milk production, weight loss, miscarriages, infertility as well significant economic losses. It vector-borne smallpox disease cattle buffalo. Characterized by appearance pustules on skin. Identification LSDV be carried out three methods, namely...

African swine fever (ASF) is a disease that emerged in Indonesia 2019 the North Sumatra province and spread rapidly to other areas, such as South Lampung, 2020. This study aimed identify phylogenetics of ASF virus (ASFV) provinces Lampung.Nine ASFV isolates collected from Disease Investigation Center Lampung were used this study. The cases 2020-2022. sequenced compared with establish genotype. Sequencing was performed using complete E183L gene target encoding p54 protein.This showed Sumatera...

Abstract DNA extraction procedures may exhibit different levels of sensitivity. A preliminary effort to obtain from captive Sumatran elephants ( Elephas maximus sumatranus) in Elephant Training Center Way Kambas National Park, Lampung for building their molecular individual identification was done. 30 male and 23 female elephants’ blood samples extracted by QIAGEN. Qualitative analysis elephant conducted using two methods, simple electrophoresis based on PCR product. The aim this project is...

Gapoktanhut Lestari Sejahtera, a community engaged in coffee plantation practices within the forests of Forest Protection Register 31 Pematang Arahan, Tanggamus, operates under forest management unit, KPH Kotaagung Utara, Lampung. In line with its commitment to biodiversity governance and preservation conservation biodiversity, species confirmation, particularly for robusta coffee, is deemed necessary. A leaf-based molecular confirmation was proposed, method yet be conducted Therefore, prior...

Stingless bees are one of the honey-producing groups that currently in increasing demand. The significant rise honey demand necessitates research into variety stingless bee species have potential to produce high-quality large quantities. This study aims determine genetic relationship from two colonies Rajabasa, Bandar Lampung. Samples were collected different Molecular analysis was conducted, including DNA extraction, amplification, sequencing, and phylogenetic using 16S rRNA gene. assessed...

Way Kambas National Park (WKNP) is home of five protected big mammals including sumatran elephants. It shares its border with 22 37 villages surrounding the national park. Understanding their existence in wild a priority, and wildlife genetics crucially needed. Besides poaching habitat fragmentation, wildlife-human conflict one issue. Elephant Training Center (ETC) WKNP built for semi in-situ conservation effort on captive elephants that mainly have histories local people. Participative...

Sumatran elephant is a subspecies of endemic Asian elephants on the island Sumatra and included in Red List International Union for Conservation Nature (IUCN) with critically endangered status. The building Elephant Training Centre (ETC) Way Kambas National Park (WKNP) one conservation efforts elephants. Small closed population size lead to an increased risk inbreeding that triggers reduction genetic variation viability increases extinction. phylogenetic pattern Indonesia has shown low...

Elephant Training Center (ETC) Way Kambas National Park (WKNP) was built to support human-elephantmitigation conflict. The small population of captive sumatran elephant in ETC WKNP need a comprehensivestrategy order maintain the genetic variation each individual and avoid inbreeding drive. Currently, geneticstudies have opened new field studies ecology, included conservation ecology. Patterns populationhas been investigated by molecular method supporting species effort. elephant’sID Card is...

Considering the increasing incidence of infectious bovine rhinotracheitis (IBR) in Indonesia, it was necessary to conduct a more in-depth study herpesvirus-1 (BHV-1) as causative agent IBR disease. Previous research reports indicate that BHV-1 subtypes found Indonesia are subtype 1.1. Currently, field case detection still uses serological method (ELISA), which has potential give false positive results and cannot explain virus subtype. Other methods, such viral isolation, take longer require...

Penelitian ini bertujuan melakukan karakterisasi molekuler antigenic site terhadap isolat virus avian influenza (AI) Balai Penyidikan dan Pengujian Veteriner (BPPV) Regional III Lampung dari tahun 2008-2013. Amplifikasi RNA dilakukan dengan teknik reverse transcription polymerase chain reaction (RT-PCR) menggunakan 4 pasang primer referens Australian Animal Health Laboratory (AAHL) Geelong Australia (HA10, HA20, HA30) dilanjutkan proses pengurutan. Analisis hasil pengurutan perangkat lunak...

Introduction: Newcastle Disease (ND) is an infectious disease in various types of poultry caused by the Virus (NDV). Cases ND Indonesia have been reported commercial and backyard chickens, pigeons, ducks geese, even eagles peacocks. Peacock a wild bird protected Indonesia's Government Regulation No. 7 1999. This study aims to identify, isolate characterize NDV molecularly cases diagnosed as Method: Samples were obtained from organs (lungs spleen) peacocks which showed neurological symptoms,...

Pesawaran is one of the areas that has potential to cultivate stingless bees as producers honey and propolis. This study was aimed at qualitatively analysing partial amplicons 16S rRNA gene from individuals found research site. The performed April September 2023 in an explorative approach. Based on exploration been done Harapan Jaya Village, Way Ratai District, Regency, individual were obtained 5 different colonies. Individual samples further extracted amplified by commercial kits. Each...

DNA detection of male sumatran elephants' (Elephas maximus sumatranus) blood samples using simple and molecular methods are ways to find out the quality from extracted samples. Molecular biology analysis was done in Biotechnology Laboratory, Lampung Disease Investigation Center, under Institution Nation Strategy Grant, Directorate General Research Empowerment Development, Ministri Research, Technology Higher Education 2018 Number 393/UN26.21/PN/2018. Sensitivity test on 12 conducted methods....

Farming is one sector that has an important role in building Indonesia's economic growth, especially for daily food. Farm production facing challenges, include the low yield, traditional management, and problems related to animal health. Infectious Bovine Rhinotracheitis (IBR) Malignant Catarrhal Fever (MCF) are diseases caused by Herpesviridae. These high mortality rate disease cause huge loss. Farms located nearby deer captivity increases risk of be infected same diseases. Surveillance...

Farming is one sector that has an important role in building Indonesia's economic growth, especially for daily food. Farm production facing challenges, include the low yield, traditional management, and problems related to animal health. Infectious Bovine Rhinotracheitis (IBR) Malignant Catarrhal Fever (MCF) are diseases caused by Herpesviridae. These high mortality rate disease cause huge loss. Farms located nearby deer captivity increases risk of be infected same diseases. Surveillance...

Sumatran elephant is declared as critically endangered by IUCN since 2011. In supporting conservation efforts on sumatran in its natural habitat, molecular data collection of their population needs to be done. Genetic source material mostly obtained habitat dung/feces. Study cytochrome B gene genetic marker from fecal samples can done get additional a form effort. Primer optimization based has been carried out an early study genetics. program was under Higher Education Applied Research...