A5047942695- Glycosylation and Glycoproteins Research
- Carbohydrate Chemistry and Synthesis
- Ocular Surface and Contact Lens
- Analytical Chemistry and Chromatography
- Mass Spectrometry Techniques and Applications
- Muscle Physiology and Disorders
- Genomics and Phylogenetic Studies
- Metabolomics and Mass Spectrometry Studies
- Bee Products Chemical Analysis
- Mitochondrial Function and Pathology
- Biochemical and Molecular Research
- Alzheimer's disease research and treatments
Institute of Polymers, Composites and Biomaterials
2015-2024
National Research Council
2020-2021
Istituto di Chimica Biomolecolare
2004
Abstract The spectra recorded by matrix‐assisted laser desorption/ionization time‐of‐flight/time‐of‐flight tandem mass spectrometry (MALDI‐TOF/TOF‐MS/MS) of complex carbohydrates from human milk are presented. Besides ions originating glycosidic cleavages and sugar ring fragmentations, these show intense peaks that may be assigned to produced three new fragmentation pathways involving a six‐atom rearrangement. These ions, together with the A fragments open possibility obtaining complete...
Deleterious variants in N- acetylneuraminate pyruvate lyase (NPL) cause skeletal myopathy and cardiac edema humans zebrafish, but its physiological role remains unknown. We report generation of mouse models the disease: Npl R63C , carrying human p.Arg63Cys variant, del116 with a 116-bp exonic deletion. In both strains, NPL deficiency causes drastic increase free sialic acid levels, reduction muscle force endurance, slower healing smaller size newly formed myofibers after cardiotoxin-induced...
Tear fluid N-Glycome from patients affected with vernal (VKC) and atopic keratoconjunctivitis (AKC) was investigated to identify specific changes in tears recognize possible glyco-biomarkers.The analysis of the N-glycans performed using matrix-assisted laser desorption ionization mass spectrometry on single tear samples. Tears control normal subjects (CTRL), VKC AKC were processed treated peptide N-glycosidase F (PNGase F) deglycosylate N-glycoproteins. Released purified, permethylated,...
Purpose Tear fluid N-Glycome from patients affected with vernal (VKC) and atopic keratoconjunctivitis (AKC) was investigated to identify specific changes in tears recognize possible glyco-biomarkers. Methods The analysis of N-glycans performed using matrix-assisted laser desorption ionization mass spectrometry on single tear samples. Tears control normal subjects (CTRL), VKC AKC were processed treated peptide N-glycosidase F (PNGase F) deglycosylate N-glycoproteins. Released purified,...