Changes in free cytosolic calcium were measured UMR-106 cells response to parathyroid hormone (PTH) stimulation.Bovine PTH-(1-34) induced an increase [Ca"+]i with the contour of rise [Ca2+li occurring three successive phases: 1) a rapid [Ca2+jj within seconds, 2) decrement [Ca2+Ii near-resting levels 1 min, and 3) slow increment [Ca2+Ji.Phase one phase increases dependent on medium calcium.The [Caz+li was inhibitable by channel blockers lanthanum verapamil.Only blocked preincubation phorbol...

The effect of prostaglandins (PG) on free cytosolic calcium concentrations [( Ca2+]i) and cAMP levels was studied in the osteosarcoma cell line UMR-106. PGF2 alpha PGE2, but not 6-keto-PGF1 alpha, induced an increase [Ca2+]i which mainly due to Ca2+ release from intracellular stores. EC50 for approximately 7 nM, whereas that PGE2 1.8 microM. Maximal doses increased higher than PGE2. Both active PGs also stimulated phosphatidylinositol turnover UMR-106 cells. effects two were independent each...

2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid [Ca*+],, free cytosolic calcium concentration; EGTA, [ethylenebis(oxyethylenenitrilo)]tetraacetic acid.

Abstract The effects of protein kinase C stimulation on free cytosolic Ca2+ [( Ca2+]i) were studied in Fura 2-loaded UMR-106 cells. Stimulation the with tumor-promoting phorbol esters 12-O-tetradecanoylphorbol 13-acetate (TPA) and 12,13-diacetate or 1-oleoyl-2-acetylglycerol was followed by an increase [Ca2+]i. C-induced [Ca2+]i has a lag period, duration which dependent stimulant medium concentrations. With 2 microM TPA, rise peaked within 1.5 min, after returned partially toward base line....

The interaction of Na and H ions with the extracellular intracellular sites Na+/H+ exchanger osteosarcoma cell line UMR-106 was investigated. interact a single, saturable transport site. H+ amiloride appear to compete Na+ for binding this apparent affinity (Nao+) independent (Hi+), Nai+, or an outwardly directed gradient. face had sigmoidal characteristic Hill coefficient approximately 2. Hi+ Nao+ between 25 140 mM. Hi+, but not number sites, increased increase in gradient across membrane....

Measurements of cell volume changes, free cytosolic Ca2+ concentration [( Ca2+]i) with Fura 2 and membrane potential 3,3'-dipropylthiodicarbocyanine iodide were used to study the effect change on influx osteoblastic osteosarcoma line, UMR-106-01. Swelling cells by hypo-osmotic stress was followed reduction in which markedly impaired removal medium Ca2+. Accordingly, swelling resulted [Ca2+]i increase only presence The swelling-activated entry pathway active at resting potentials, through...

The sre gene (ORF469) of the R4 phage encodes a protein similar to resolvase-DNA invertase family proteins. Insertional disruption prevented lysogen from entering lytic cycle, implying that Sre is site-specific recombinase needed for excision prophage genome (M. Matsuura, T. Noguchi, Aida, M. Asayama, H. Takahashi, and Shirai, J. Gen. Appl. Microbiol. 41:53-61, 1995). To determine whether this also necessary integration reaction, we studied its function by plasmid analysis. When deletions,...

Measurements of cytosolic pH (pHi) 36Cl fluxes and free Ca2+ concentration ([Ca2+]i) were performed in the clonal osteosarcoma cell line UMR-106 to characterize kinetic properties Cl-/HCO3- (OH-) exchange its regulation by pHi [Ca2+]i. Suspending cells Cl(-)-free medium resulted rapid alkalinization from 7.05 approximately 7.42. Subsequently, cytosol acidified 7.31. Extracellular HCO3- increased rate extent prevented secondary acidification. alkalinized Cl(-)-depleted Cl(-)-containing...

N-Terminal analogs of PTH-related protein (PTHrP) and PTH bind to a common receptor exhibit similar biological properties. However, recent studies suggest that certain midregion C-terminal PTHrP peptides have activities distinct from those in the placenta osteoclasts, respectively. In this study we determined full-length recombinant PTHrP-(1-141) several synthetic N-terminal, midregion, fragments two PTHrP-producing cell types. Peptides were tested for their ability stimulate cAMP production...

SUMMARY The regulatory role of chemokines and chemokine receptors on specific lymphocyte recruitment into periodontal diseased tissue is poorly characterized. We observed that lymphocytes infiltrating inflamed gingival expressed marked levels CCR6. In tissue, the expression MIP-3α mRNA was detected by RT-PCR further, distributed in basal layer epithelial cells, microvascular endothelial cells areas inflammatory as shown immunohistochemistry. Moreover, CCR6-expressing infiltrated proportion...

The naturally occurring prostaglandins (PGs) were studied with respect to their abilities change free cytosolic Ca2+ concentrations ([Ca2+]i), adenosine 3',5'-cyclic monophosphate (cAMP) levels, and cell proliferation in the osteoblastic line, UMR-106-01, primary cultures of osteoblasts prepared from neonatal rat calvariae. All PGs tested stimulated an increase [Ca2+]i, which was mainly due release intracellular stores. Measurements 50% effective concentration for different show that potency...

The effect of volume perturbation on the interaction Na+ and H+ with intracellular extracellular faces Na+/H+ exchanger was studied in UMR-106 cells, a rat osteosarcoma cell line. Osmotic shrinkage cells stimulated activity exchanger. Kinetic analysis this stimulation demonstrated that hyperosmotically stressed apparent affinities for are modified opposite directions. While there is an increased affinity protons from 0.275 +/- 0.03 to 0.107 0.025 microM isotonic hypertonic conditions,...

Abstract. Generally, fibroblast‐like cells and other types of human have been used to demonstrate the principles replicative senescence in vitro vivo . These go through three stages proliferation, including vigorous declining proliferation quiescence or no proliferation. Any variation this process occurring osteoprogenitor may offer insight into mechanism age‐related osteopaenia that predisposes individuals osteoporosis bone fractures. We selected MC3T3‐E1 derived from mouse calvaria study...

Abstract The target‐cell limited model, which is one of the mathematical modeling approaches providing a quantitative understanding viral dynamics, has been applied to describe RNA profiles severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) in previous studies. However, these models have developed mainly using patient data from early phase pandemic. Furthermore, no reports focused on titer. In this study, dynamics both and titer were characterized reflecting current clinical...

Summary The regulatory role of chemokines and chemokine receptors on specific leucocyte recruitment into periodontal diseased tissue is poorly characterized. We observed that leucocytes infiltrating inflamed gingival expressed marked levels CX3CR1. In tissue, the expression fractalkine CX3CR1 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) further, distributed mainly endothelial cells, as shown immunohistochemistry. Moreover, we can detect CX3CR1-expressing...

A wide spectrum of prostaglandins (PG) stimulate both the production cyclic AMP and an increase in free cytosolic Ca2+ concentration [(Ca2+]i) osteogenic osteosarcoma cell line, UMR-106-01, which has characteristics compatible with osteoblasts. Using PG-stimulated determinations second messengers [Ca2+]i, a method for classification PG receptors is presented. UMR-106-01 cells demonstrate three subclasses receptors. One receptor interacts PGF2 alpha, PGD2, thromboxane B2 (TxB2) to [Ca2+]i....

Abstract The potential role of ELR + CXC chemokines in early events bone repair was studied using human mesenchymal stem cells (hMSCs). Inflammation, which occurs the initial phase tissue healing general, is critical to repair. Release cytokines from infiltrating immune and injured can lead recruitment MSCs region bearing Glu‐Leu‐Arg (ELR) motif are also released by inflammatory serve as angiogenic factors stimulating chemotaxis proliferation endothelial cells. hMSCs, induced differentiate...

Tumor necrosis factor (TNF-alpha) has been shown to play an important role in local control of bone remodeling. The interaction TNF-alpha and PTH was evaluated UMR-106-01 cells, a phenotypic osteoblastic osteosarcoma cell line. We examined the influence on two signal transduction systems triggered by adenylate cyclase free cytosolic calcium ([Ca2+]i). cAMP generation inhibited TNF-alpha-pretreated cells 69, 61, 34, 21% at concentrations 0.1, 1, 10, 100 nM, respectively. Inhibition seen doses...

Activation of sodium/proton (Na+/H+) antiport activity has been shown to occur as an early event in mitogenesis. Because amiloride inhibits Na+/H+ activity, it is hypothesized that mitogenesis may be inhibited by amiloride. In this work, we examined the effect on DNA synthesis measured [3H]thymidine uptake and immunoglobulin (Ig) production ELISA system human peripheral blood mononuclear cells (PBM). Amiloride at 100 microM concentration irradiated Raji cell (*R)-activated...

Abstract When planning pediatric clinical trials, optimizing the sample size of neonates/infants is essential because it difficult to enroll these subjects. In this simulation study, we evaluated using a model‐based optimal approach for identifying their pharmacokinetics cefiderocol. We assessed usefulness data estimation performance (accuracy and variance parameter estimation) from adults impact very young subjects, including preterm neonates. Stochastic were utilized assess allocation age...

Mathematical modeling can provide quantitative understanding of the viral dynamics and reduction effects drugs enable simulations in various scenarios. In this study, a drug effect model ensitrelvir was developed to describe effect. Using model, we also estimated impact treatment with on number infected patients at population level Japan. The based dynamic for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pharmacokinetic using 10,477 samples load from 1447 disease 2019...

Abstract. The classical models of in vitro cell culture comprise fibroblasts and epithelial cells. Osteogenic cells represent another interesting model; however, it is not known whether during osteogenesis density regulates growth as seen cultures We selected MC3T3‐E1 for study because they are an osteogenic line that, when subcultured, grow to confluence form multilayers conventional by continued proliferation, do fibroblasts. Once maximum obtained, proliferation down regulated resulting a...