A5074069120- Protein purification and stability
- Bacterial Genetics and Biotechnology
- Monoclonal and Polyclonal Antibodies Research
- Enzyme Production and Characterization
- Virus-based gene therapy research
- Protein Structure and Dynamics
- Advanced biosensing and bioanalysis techniques
- Metabolomics and Mass Spectrometry Studies
- Molecular Sensors and Ion Detection
- Bacteriophages and microbial interactions
- Pancreatic function and diabetes
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cannabis and Cannabinoid Research
- Enzyme Structure and Function
- Diet, Metabolism, and Disease
- Genomics and Phylogenetic Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Microplastics and Plastic Pollution
- Electrochemical sensors and biosensors
- Enzyme Catalysis and Immobilization
- Transgenic Plants and Applications
- RNA Interference and Gene Delivery
- Effects and risks of endocrine disrupting chemicals
- Mass Spectrometry Techniques and Applications
- Dyeing and Modifying Textile Fibers
TU Wien
2021-2024
Cannabidiol (CBD) has received great scientific interest due to its numerous therapeutic applications. Degradation in the gastrointestinal (GI) tract, first-pass metabolism, and low water solubility restrain bioavailability of CBD only 6% current oral administration. Lipid-based nanocarriers are delivery systems that may enhance accessibility hydrophobic payloads, such as CBD. Conventional lecithin-derived liposomes, however, have limitations regarding stability GI tract long-term storage....
Neutral and positively charged archaeal ether lipids (AEL) have been studied for their utilization as novel delivery systems pDNA, showing efficient immune response with a strong memory effect while lacking noticeable toxicity. Recent technological advances placed mRNA lipid nanoparticles (LNPs) at the forefront of next-generation systems; however, no study has examined AELs in yet. In this study, we investigated either crude extract or purified tetraether caldarchaeol from Sulfolobus...
Abstract The heterotrophic cultivation of extremophilic archaea still heavily relies on complex media. However, media are associated with unknown composition, high batch-to-batch variability, potential inhibiting and interfering components, as well regulatory challenges, hampering advancements in genetic engineering bioprocessing. For Metallosphaera sedula , a widely studied organism for biomining bioremediation production host archaeal ether lipids, efforts to find defined conditions have...
Textile waste is mostly incinerated because few recycling processes are available to recover valuable materials. In this work, a feasible chemo-enzymatic process of wool/polyethylene terephthalate (PET)/elastane blends pure PET for the first time successfully demonstrated. Two novel enzyme formulations were selected wool hydrolysis, whereas recovered amino acids quantified using high-performance liquid chromatography and two assays (Ninhydrin Folin–Ciocalteu). Kinetic studies on acid...
A microfluidic platform with immobilized human flavin-containing monooxygenase for high-throughput screening of drugs relevance to personalised medicine.
Overflow metabolism is a well-known phenomenon that describes the seemingly wasteful and incomplete substrate oxidation by aerobic cells, such as yeasts, bacteria, mammalian even when conditions allow for total combustion via respiration. This cellular response, triggered an excess of C-source, has not yet been investigated in archaea. In this study, we conducted chemostat cultivations to compare metabolic physiological states thermoacidophilic archaeon Sulfolobus acidocaldarius under three...
Abstract BACKGROUND Downstream processing of soluble recombinant proteins from Escherichia coli is complicated by the need to access intracellular product cell disruption and separate target protein impurities, particularly host protein, DNA, endotoxins lipids. We previously demonstrated ability E. X‐press strain leak high amounts culture medium without sacrificing viability. In this case study, we assessed economic ecological benefit for downstream in direct comparison industrial standard...