A5072029578- Genomics and Phylogenetic Studies
- Molecular Biology Techniques and Applications
- MicroRNA in disease regulation
- Monoclonal and Polyclonal Antibodies Research
- Platelet Disorders and Treatments
- Bacteriophages and microbial interactions
- Dermatology and Skin Diseases
- Protease and Inhibitor Mechanisms
- Circular RNAs in diseases
- Evolution and Genetic Dynamics
- Reproductive Biology and Fertility
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Nanopore and Nanochannel Transport Studies
- Immune Response and Inflammation
- RNA and protein synthesis mechanisms
- Insect and Arachnid Ecology and Behavior
- Blood Coagulation and Thrombosis Mechanisms
- Neurobiology and Insect Physiology Research
- Insect Resistance and Genetics
- Cell Adhesion Molecules Research
- Environmental DNA in Biodiversity Studies
- Veterinary Equine Medical Research
- Invertebrate Immune Response Mechanisms
- Metabolism and Genetic Disorders
Ghent University
2016-2025
Pharmaceutical Biotechnology (Czechia)
2020
D2S International (Belgium)
1992-1997
Vlaams Instituut voor Biotechnologie
1997
KU Leuven
1993
Whole genome sequence analysis (digital DNA-DNA hybridization and average nucleotide identity) was carried out for 81 sequenced full genomes of the genus Gardnerella, including ten determined in this study, indicated existence 13 genomic species, which five consist only one strain contain more than four genomes. Furthermore, a collection Gardnerella strains, representing emended species G. vaginalis newly described leopoldii, piotii swidsinskii, studied. Matrix-assisted laser desorption...
Hookworms are hematophagous nematodes that infect a wide range of mammalian hosts, including humans. There has been speculation for nearly century as to the identity anticoagulant substances) used by these organisms subvert host hemostasis. Using molecular cloning, we describe family potent small protein (75-84 amino acids) anticoagulants from hookworm Ancylostoma caninum termed AcAP (A. protein). Two recombinant members (AcAP5 and AcAP6) directly inhibited catalytic activity blood...
ABSTRACT The enediyne family of natural products has had a profound impact on modern chemistry, biology, and medicine, yet only 11 enediynes have been structurally characterized to date. Here we report genome survey 3,400 actinomycetes, identifying 81 strains that harbor genes encoding the polyketide synthase cassettes could be grouped into 28 distinct clades based phylogenetic analysis. Genome sequencing 31 representative confirmed each clade harbors biosynthetic gene cluster. A...
Bacteriophages are a promising therapeutic strategy among cystic fibrosis and lung-transplanted patients, considering the high frequency of colonization/infection caused by pandrug-resistant bacteria. However, little clinical data available regarding use phages for infections with Achromobacter xylosoxidans. A 12-year-old patient received two rounds phage therapy because persistent lung infection A. Clinical tolerance was perfect, but initial bronchoalveolar lavage (BAL) still grew The...
Significance Leinamycin (LNM) is a promising anticancer drug lead, yet no analog has been isolated since its discovery nearly 30 y ago. By mining bacterial genomes, we discovered 49 potential producers of LNM-type natural products, the structural diversity which was predicted based on bioinformatics and confirmed by in vitro characterization selected enzymes elucidation guangnanmycins weishanmycins. These findings demonstrate power discovery-based approach to combinatorial biosynthesis for...
We describe a comparative sequencing strategy that is based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analyses of complete base-specific cleavage reactions target sequence. The converted to DNA/RNA mosaic structure after PCR amplification using in vitro transcription. Cleavage with defined specificity achieved by ribonucleases. set products subjected without prior fractionation. presented resequencing assay particularly useful for...
One of the latest developments in next generation sequencing is Oxford Nanopore Technologies' (ONT) MinION nanopore sequencer. We studied applicability this system to perform forensic genotyping female DNA standard 9947 A using 52 SNP-plex assay developed by SNPforID consortium. All but one loci were correctly genotyped. Several SNP identified as problematic for correct and robust sequencing. these contained homopolymers sequence flanking most them already reported studies other...
Double-stranded RNA (dsRNA) molecules of viral origin trigger a post-transcriptional gene-silencing mechanism called interference (RNAi). Specifically, virally derived dsRNA is recognized and cleaved by the enzyme Dicer2 into short interfering RNAs (siRNAs), which further direct sequence-specific silencing, ultimately silencing replication virus. Notably, RNAi can also be artificially triggered delivery gene-specific dsRNA, thereby leading to endogenous gene silencing. This widely used...
Most therapeutic proteins in clinical trials or on the market are, to a variable extent, immunogenic. Formation of antidrug antibodies poses risk that should be assessed during drug development, as it possibly compromises safety and alters pharmacokinetics. The generation these is critically dependent presence T helper cell epitopes, which have classically been identified by vitro methods using blood cells from human donors. As novel silico identify epitopes coming line. These are relatively...
Nanopore sequencing for forensic short tandem repeats (STR) genotyping comes with the advantages associated massively parallel (MPS) without need a high up-front device cost, but is inaccurate, partially due to occurrence of homopolymers in STR loci. The goal this study was apply latest progress nanopore by Oxford Technologies field genotyping. experiments were performed using state art R9.4 flow cell and most recent R10 cell, which specifically designed improve consensus accuracy...
<ns3:p><ns3:bold>Background</ns3:bold>: At the time of publication, most devastating desert locust crisis in decades is affecting East Africa, Arabian Peninsula and South-West Asia. The situation extremely alarming where Kenya, Ethiopia Somalia face an unprecedented threat to food security livelihoods. Most time, however, locusts do not occur swarms, but live as relatively harmless solitary insects. phenotypically distinct solitarious gregarious phases differ markedly many aspects behaviour,...
<ns3:p><ns3:bold>Background</ns3:bold>: At the time of publication, most devastating desert locust crisis in decades is affecting East Africa, Arabian Peninsula and South-West Asia. The situation extremely alarming where Kenya, Ethiopia Somalia face an unprecedented threat to food security livelihoods. Most time, however, locusts do not occur swarms, but live as relatively harmless solitary insects. phenotypically distinct solitarious gregarious phases differ markedly many aspects behaviour,...
Abstract Background Within the follicular fluid, extracellular vesicles (EVs) guide oocyte growth through their cargo microRNAs (miRNAs). Here, we investigated role of EVs and miRNAs by linking found in EVs, derived from fluid an individual follicle, to ability its become a blastocyst (competent) or not (non-competent). Methods Bovine antral follicles were dissected, categorized as small (2–4 mm) large (5–8 corresponding oocytes subjected maturation, fertilization embryo culture stage....
The potential and current state-of-the-art of forensic SNP genotyping using nanopore sequencing was investigated with a panel 16 tri-allelic single nucleotide polymorphisms (SNPs), multiplexing five samples per run. sample set consisted three single-source human genomic reference control DNA two GEDNAP samples, simulating casework samples. primers for the multiplex SNP-loci PCR were taken from study which researched their value in setting conventional single-base extension technology....
Recently, secreted microRNAs (miRNAs) have received a lot of attention since they may act as autocrine factors. However, how miRNAs influence embryonic development is still poorly understood. We identified 294 miRNAs, 114 known, and 180 novel, in the conditioned medium individually cultured bovine embryos. Of these miR-30c miR-10b were much more abundant slow cleaving embryos compared to intermediate ones. MiR-10b, miR-novel-44, miR-novel-45 higher expressed degenerate blastocysts, while...
Diet-induced obesity can alter ovarian functions after long-term high-fat high-sugar (HFHS) diet feeding. However, the acute impact of obesogenic diets on follicular cells, and how this evolves over time when intake is continued develops, not known. Our aim was to determine onset progression changes in granulosa cell (GC) transcriptomic profile starting a HFHS-diet feeding mice. We also examined oocyte lipid droplet content (LDC) mitochondrial ultrastructural abnormalities. Swiss (outbred)...
Aleurone, derived from the bran layer of grains like wheat and barley, has demonstrated positive effects on energy metabolism in pigs, mice, untrained horses, influencing glucose-insulin dynamics gut microbiome composition. Training itself enhances insulin sensitivity similar to improvements performance capacity observed human athletes. This study aimed investigate whether aleurone supplementation provides additional benefits training by modulating microbiota Standardbred mares. Sixteen...
Understanding genetic responses to forest dynamics is essential for predicting the long-term viability of understory plant populations and developing effective conservation strategies. This study investigates extinction debt colonization credit in Circaea lutetiana, a clonal species, across its European range. Using pooled genotype-by-sequencing data from 40 edge core populations, we examined what extent population size, latitude historical changes configuration predict diversity. Our...
A recombinant single-chain molecule, scFv-K12G0, containing the variable domains of monoclonal antibody MA-15C5, specific for fragment D-dimer human cross-linked fibrin, was constructed and expressed in Spodoptera frugiperda, Sf9, insect cells. The Arg108 carboxyl-terminal amino acid domain light-chain connected through a synthetic Ala-Gly-Gln-Gly-Ser-Ser-Val peptide linker with Gln1 amino-terminal its heavy chain. scFv-K12G0 secreted by infected Sf9 cells at rate 10 micrograms/10(6) within...
Enchytraeids (Annelida) are soil invertebrates with worldwide distribution that have served as ecotoxicology models for over 20 years. We present the first high-quality reference genome of Enchytraeus crypticus, assembled from a combination Pacific Bioscience single-molecule real-time and Illumina sequencing platforms 525.2 Mbp (910 gapless scaffolds 18,452 genes). highlight isopenicillin, acquired by horizontal gene transfer conferring antibiotic function. Significant family expansions...
There is a great need for objective external training load prescription and performance capacity evaluation in equestrian disciplines. Therefore, reliable standardised exercise tests (SETs) are needed. Classic SETs require maximum intensities with associated risks to deduce loads from pre-described cut-off values. The lactate minimum speed (LMS) test could be valuable alternative. Our aim was compare new parameters of modified LMS-test those an incremental SET, assess the effect on...
Loss-of-function mutations in the deubiquitinase OTULIN result an inflammatory pathology termed “OTULIN-related autoinflammatory syndrome” (ORAS). Genetic mouse models revealed essential roles for and cell death signaling, but mechanisms by which deficiency connects to inflammation remain unclear. Here, we identify as a cellular condition that licenses RIPK3-mediated murine macrophages, leading Nlrp3 inflammasome activation subsequent IL-1β secretion. uncoupled from gasdermin D–mediated...