A5059284802- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Capillary Electrophoresis Applications
- Single-cell and spatial transcriptomics
- Renal and related cancers
- Cancer Genomics and Diagnostics
- Monoclonal and Polyclonal Antibodies Research
- Nanofabrication and Lithography Techniques
- Reproductive System and Pregnancy
- Reproductive Biology and Fertility
- Gene expression and cancer classification
- CRISPR and Genetic Engineering
- Extracellular vesicles in disease
- Immune Cell Function and Interaction
- Microfluidic and Bio-sensing Technologies
- Genomics and Phylogenetic Studies
Ghent University Hospital
2025
Ghent University
2022-2024
Abstract Background Within the follicular fluid, extracellular vesicles (EVs) guide oocyte growth through their cargo microRNAs (miRNAs). Here, we investigated role of EVs and miRNAs by linking found in EVs, derived from fluid an individual follicle, to ability its become a blastocyst (competent) or not (non-competent). Methods Bovine antral follicles were dissected, categorized as small (2–4 mm) large (5–8 corresponding oocytes subjected maturation, fertilization embryo culture stage....
CYP2D6 is a very important pharmacogene as it responsible for the metabolization or bioactivation of 20 to 30% clinically used drugs. However, despite its relatively small length only 4.4 kb, one most challenging pharmacogenes genotype due high similarity with neighboring pseudogenes and frequent occurrence CYP2D6-CYP2D7 hybrids. Unfortunately, current genotyping methods are therefore not able correctly determine complete sequence. Therefore, we developed assay generate allele-specific...
<title>Abstract</title> While contemporary short-read single cell RNA-sequencing allows to decipher tissue composition, discrimination between transcript isoforms remains challenging. Here, we propose long-read isoform sequencing (scLIS-seq), and highlight its performance on Jurkat HEK293T cells in direct comparison Smart-seq3xpress (SS3X). scLIS-seq demonstrates sensitive gene detection with high correlation compared SS3X detects at least 10 of over 2600 genes, while 17.1–21.6% the reads...
Pharmacogenomics (PGx) studies the impact of interindividual genomic variation on drug response, allowing opportunity to tailor dosing regimen for each patient. Current targeted PGx testing platforms are mainly based microarray, polymerase chain reaction, or short-read sequencing. Despite demonstrating great value identification single nucleotide variants (SNVs) and insertion/deletions (INDELs), these assays do not permit large structural variants, nor they allow unambiguous haplotype...
Monoclonal antibodies (mAbs) hold significant potential as therapeutic agents and are invaluable tools in biomedical research. However, the lack of efficient high-throughput screening methods for single antibody-secreting cells (ASCs) has limited diversity available antibodies. Here, we introduce a novel, integrated workflow employing self-seeding microwells an automated microscope-puncher system swift, isolation ASCs. The allows individual up to 6,400 within approximately one day, with...
Abstract CYP2D6 is one of the most challenging pharmacogenes to genotype due high similarity with its neighboring pseudogenes and frequent occurrence CYP2D6-CYP2D7 hybrids. Unfortunately, current genotyping methods are therefore not able correctly determine complete sequence. Therefore, we developed a assay generate allele-specific consensus sequences complex regions by optimizing PCR-free nanopore Cas9-targeted sequencing (nCATS) method combined adaptive sequencing, developing new...