A5006568175- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Mesenchymal stem cell research
- Monoclonal and Polyclonal Antibodies Research
- Cancer Cells and Metastasis
- interferon and immune responses
- Cell Adhesion Molecules Research
- Virology and Viral Diseases
- Animal health and immunology
- COVID-19 Clinical Research Studies
- Tissue Engineering and Regenerative Medicine
- Microfluidic and Bio-sensing Technologies
- Viral Infections and Immunology Research
- Hematopoietic Stem Cell Transplantation
- COVID-19 epidemiological studies
- vaccines and immunoinformatics approaches
- Nanofabrication and Lithography Techniques
- HIV Research and Treatment
Ghent University
2014-2024
Ghent University Hospital
2022
Porcine epidemic diarrhea virus (PEDV) is a member of the family Coronaviridae and can cause severe outbreaks in piglets from different age groups. Here, we report complete genome sequence (28,028 nt) PEDV strain isolated during novel outbreak Belgium.
Feline infectious peritonitis (FIP) results from mutations in the viral genome during a common feline enteric coronavirus (FECV) infection. Since many virological and immunological data on FECV infections are lacking, present study investigated these missing links experimental infection of three SPF cats with strain UCD. Two showed mild clinical signs, faecal shedding virus 4 dpi, cell-associated viraemia at inconsistent time points 5 highly neutralising antibody response 9 no major...
Despite the high efficacy of BNT162b2 vaccine in general population, data on its immunogenicity among frail elderly individuals are limited. Recently, levels anti-SARS-CoV-2 spike IgG antibodies and serum neutralization titers were confirmed as good immune markers protection against virus, with evidence showing a reverse correlation between these two parameters susceptibility to infection. Here we analyzed sera from 138 nursing home residents (median age 88.9 years) 312 staff 50.7 determine...
To initiate infections, many coronaviruses use sialic acids, either as receptor determinants or attachment factors helping the virus find its underneath heavily glycosylated mucus layer. In present study, role of acids in serotype I feline enteric coronavirus (FECV) infections was studied intestinal epithelial cell cultures. Treatment cells with neuraminidase (NA) enhanced infection efficiency, showing that terminal acid residues on surface were not and even hampered efficient virus–receptor...
One of the most characteristic pathological changes in cats that have succumbed to feline infectious peritonitis (FIP) is a multifocal granulomatous phlebitis. Although it now well established leukocyte extravasation elicits inflammation typically associated with FIP lesions, relatively few studies aimed at elucidating this key pathogenic event. The upregulation adhesion molecules on endothelium prerequisite for stable leukocyte–endothelial cell (EC) necessarily precedes diapedesis....
Mesenchymal stromal cells have been isolated from different sources. They are multipotent capable of differentiating into many cell types, including osteocytes, chondrocytes and adipocytes. possess a therapeutic potential in the management immune disorders repair damaged tissues. Previous work our laboratory showed an increase percentages CD172a+, CD14+, CD163+, Siglec-1+, CD4+ CD8+ hematopoietic cells, when co-cultured with immortalized mesenchymal derived bone marrow. The present aimed to...
Feline coronavirus (FCoV) has been identified as the aetiological agent of feline infectious peritonitis (FIP), a highly fatal systemic disease in cats. FCoV open reading frame 3 (ORF3) encodes accessory proteins 3a, 3b and c. The protein consists 72 amino acid residues localizes to nucleoli mitochondria. present work focused on peptide domains within that drive its intracellular trafficking. Transfection different cell types with fused enhanced green fluorescent (EGFP) or 3×FLAG confirmed...
Feline infectious peritonitis (FIP) is a fatal disease caused by feline virus (FIPV). At present, neither licensed treatment nor an accurate ante-mortem diagnosis are available. In the present study, three available tests were evaluated for their diagnostic power on effusion samples. High coronavirus antibody titers, measured with immunoperoxidase monolayer assay (IPMA), correlated FIP but its low specificity precluded reliable diagnosis. The in-house 5’ reverse transcriptase-quantitative...
Monoclonal antibodies (mAbs) hold significant potential as therapeutic agents and are invaluable tools in biomedical research. However, the lack of efficient high-throughput screening methods for single antibody-secreting cells (ASCs) has limited diversity available antibodies. Here, we introduce a novel, integrated workflow employing self-seeding microwells an automated microscope-puncher system swift, isolation ASCs. The allows individual up to 6,400 within approximately one day, with...