Hannah Eeckhaut
A5040853997- Monoclonal and Polyclonal Antibodies Research
- RNA and protein synthesis mechanisms
- Viral Infectious Diseases and Gene Expression in Insects
- SARS-CoV-2 and COVID-19 Research
- Transgenic Plants and Applications
- Fungal and yeast genetics research
- Machine Learning in Bioinformatics
- Animal Genetics and Reproduction
- Viral gastroenteritis research and epidemiology
- vaccines and immunoinformatics approaches
- Advanced Proteomics Techniques and Applications
- CRISPR and Genetic Engineering
- Bacterial Genetics and Biotechnology
Vlaams Instituut voor Biotechnologie
2024
Ghent University
2018-2024
VIB-UGent Center for Medical Biotechnology
2018-2024
Abstract The industrial yeast Komagataella phaffii (formerly named Pichia pastoris ) is commonly used to synthesize recombinant proteins, many of which are as human therapeutics or in food. However, the basic strain, NRRL Y-11430, from all commercial hosts derived, not available without restrictions on its use. Comparative genome sequencing leaves little doubt that Y-11430 derived a K. type strain deposited UC Davis Phaff Yeast Strain Collection 1954. We analysed four equivalent strains...
Broadly neutralizing antibodies are an important treatment for individuals with coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2). Antibody-based therapeutics also essential pandemic preparedness against future Sarbecovirus outbreaks. Camelid-derived single domain (VHHs) exhibit potent antimicrobial activity and being developed as SARS-CoV-2–neutralizing antibody-like therapeutics. Here, we identified VHHs that neutralize both SARS-CoV-1...
Abstract We have identified camelid single-domain antibodies (VHHs) that cross-neutralize SARS-CoV-1 and −2, such as VHH72, which binds to a unique highly conserved epitope in the viral receptor-binding domain (RBD) is difficult access for human antibodies. Here, we establish protein engineering path how stable, long-acting drug candidate can be generated out of VHH building block. When fused IgG1-Fc, prototype VHH72 molecule prophylactically protects hamsters from SARS-CoV-2. In addition,...
Abstract While transcriptome- and proteome-wide technologies to assess processes in protein biogenesis are now widely available, we still lack global approaches assay post-ribosomal events, particular those occurring the eukaryotic secretory system. We here develop a method, SECRiFY, simultaneously secretability of >10 5 fragments by two yeast species, S. cerevisiae P. pastoris , using custom fragment libraries, surface display sequencing-based readout. Screening human proteome with...
Abstract In the standard toolkit for recombinant protein expression, yeast known in biotechnology as Pichia pastoris (formally: Komagataella phaffii ) takes up position between E. coli and HEK293 or CHO mammalian cells, is used by thousands of laboratories both academia industry. The organism eukaryotic yet microbial, grows to extremely high cell densities while secreting proteins into its fully defined growth medium, using very well established strong inducible constitutive promoters. Many...
Abstract Monoclonal antibodies are the leading drug of biopharmaceutical market because their high specificity and tolerability, but current CHO-based manufacturing platform remains expensive time-consuming to limited accessibility, especially in case diseases with incidence pandemics. Therefore, there is an urgent need for alternative production system. In this study, we present a rapid cost-effective microbial heavy chain-only (VHH-Fc) methylotrophic yeast Komagataella phaffii (aka Pichia...
Abstract While transcriptome- and proteome-wide technologies to assess processes in protein biogenesis are now widely available, we still lack global approaches assay post-ribosomal events, particular those occurring the eukaryotic secretory system. We here developed a method, SECRiFY, simultaneously secretability of >10 5 fragments by two yeast species, S. cerevisiae P. pastoris, using custom fragment libraries, surface display sequencing-based readout. Screening human proteome with...