A5011435721- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Monoclonal and Polyclonal Antibodies Research
- Bacterial Genetics and Biotechnology
- CRISPR and Genetic Engineering
- SARS-CoV-2 and COVID-19 Research
- Biochemical and Molecular Research
- Diagnosis and treatment of tuberculosis
- Fungal and yeast genetics research
- Immunodeficiency and Autoimmune Disorders
- Viral Infectious Diseases and Gene Expression in Insects
- Scientific Computing and Data Management
- Viral gastroenteritis research and epidemiology
- Immune responses and vaccinations
- Transgenic Plants and Applications
- RNA and protein synthesis mechanisms
- Carbohydrate Chemistry and Synthesis
- Bacteriophages and microbial interactions
VIB-UGent Center for Medical Biotechnology
2019-2024
Ghent University
2019-2022
Abstract The industrial yeast Komagataella phaffii (formerly named Pichia pastoris ) is commonly used to synthesize recombinant proteins, many of which are as human therapeutics or in food. However, the basic strain, NRRL Y-11430, from all commercial hosts derived, not available without restrictions on its use. Comparative genome sequencing leaves little doubt that Y-11430 derived a K. type strain deposited UC Davis Phaff Yeast Strain Collection 1954. We analysed four equivalent strains...
Broadly neutralizing antibodies are an important treatment for individuals with coronavirus disease 2019 (COVID-19) caused by severe acute respiratory syndrome 2 (SARS-CoV-2). Antibody-based therapeutics also essential pandemic preparedness against future Sarbecovirus outbreaks. Camelid-derived single domain (VHHs) exhibit potent antimicrobial activity and being developed as SARS-CoV-2–neutralizing antibody-like therapeutics. Here, we identified VHHs that neutralize both SARS-CoV-1...
Mycobacterium bovis bacillus Calmette-Guérin (M. BCG) is the only vaccine available against tuberculosis (TB). In an effort to standardize production, three substrains, i.e. BCG Danish 1331, Tokyo 172-1 and Russia BCG-1 were established as WHO reference strains. Both for BCG-1, genomes exist, not Danish. this study, we set out determine completely assembled genome sequence establish a workflow characterization of engineering-derived candidate strains.By combining second (Illumina) third...
Abstract We have identified camelid single-domain antibodies (VHHs) that cross-neutralize SARS-CoV-1 and −2, such as VHH72, which binds to a unique highly conserved epitope in the viral receptor-binding domain (RBD) is difficult access for human antibodies. Here, we establish protein engineering path how stable, long-acting drug candidate can be generated out of VHH building block. When fused IgG1-Fc, prototype VHH72 molecule prophylactically protects hamsters from SARS-CoV-2. In addition,...
The major global health threat tuberculosis is caused by Mycobacterium tuberculosis. M. has a complex cell envelope-a partially covalently linked composite of polysaccharides, peptidoglycan, and lipids, including mycolic acid layer-which conveys pathogenicity but also protects against antibiotics. Given previous successes in treating Gram-positive -negative infections with wall-degrading enzymes, we investigated such an approach for In this study, aimed to (i) develop microtiter growth...
Mutant resources are essential to improve our understanding of the biology slow-growing mycobacteria, which include causative agents tuberculosis in various species, including humans. The generation deletion mutants mycobacteria a gene-by-gene approach order make genome-wide ordered mutant is still laborious and costly approach, despite recent development improved methods. On other hand, transposon mutagenesis combination with Cartesian pooling-coordinate sequencing (CP-CSeq) allows creation...
Abstract In the standard toolkit for recombinant protein expression, yeast known in biotechnology as Pichia pastoris (formally: Komagataella phaffii ) takes up position between E. coli and HEK293 or CHO mammalian cells, is used by thousands of laboratories both academia industry. The organism eukaryotic yet microbial, grows to extremely high cell densities while secreting proteins into its fully defined growth medium, using very well established strong inducible constitutive promoters. Many...
ABSTRACT Mycobacterium bovis bacillus Calmette-Guérin ( M. BCG) is the only vaccine available against tuberculosis (TB). This study reports on an integrated genome analysis workflow for BCG, resulting in completely assembled sequence of BCG Danish 1331 (07/270), one WHO reference strains vaccines. We demonstrate how this enables resolution duplications and engineered derivatives strain.
Abstract Mutant resources are essential to improve our understanding of the biology slow-growing mycobacteria, which include causative agents tuberculosis in various species, including humans. The generation deletion mutants mycobacteria a gene-by-gene approach order make genome-wide ordered mutant is still laborious and costly approach; despite recent development improved methods. On other hand, transposon mutagenesis combination with Cartesian Pooling-Coordinate Sequencing allows creation...
Synopsis Background The major global health threat tuberculosis is caused by Mycobacterium (Mtb). Mtb has a complex cell envelope – partially covalently linked composite of polysaccharides, peptidoglycan and lipids, including mycolic acid layer which conveys pathogenicity but also protects against antibiotics. Given previous successes in treating gram-positive -negative infections with wall degrading enzymes, we investigated such approach for Mtb. Objectives (i) Development an microtiter...