A5088267443- Cellular transport and secretion
- Photosynthetic Processes and Mechanisms
- Connective tissue disorders research
- Analytical chemistry methods development
- Plant Molecular Biology Research
- Trace Elements in Health
- Viral Infectious Diseases and Gene Expression in Insects
- Fungal and yeast genetics research
- Ion-surface interactions and analysis
- CRISPR and Genetic Engineering
- Death, Funerary Practices, and Mourning
- Immune responses and vaccinations
- Hypertrophic osteoarthropathy and related conditions
- Skin Diseases and Diabetes
- Electron and X-Ray Spectroscopy Techniques
- Lignin and Wood Chemistry
- Meta-analysis and systematic reviews
- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Inflammasome and immune disorders
- Plant tissue culture and regeneration
- Medical and Biological Sciences
- Mitochondrial Function and Pathology
- Digital Imaging in Medicine
- MicroRNA in disease regulation
Ghent University
2016-2024
VIB-UGent Center for Inflammation Research
2017-2023
Ablynx (Belgium)
2023
VIB-UGent Center for Plant Systems Biology
2017-2018
Ghent University Hospital
2017-2018
Vlaams Instituut voor Biotechnologie
2016
Polyploidization has played a key role in plant breeding and crop improvement. Although its potential to increase biomass yield is well described, the effect of polyploidization on composition largely remained unexplored. Here, we generated series Arabidopsis (Arabidopsis thaliana) plants with different somatic ploidy levels (2n, 4n, 6n, 8n) performed rigorous phenotypic characterization. Kinematic analysis showed that polyploids developed slower compared diploids; however, tetra-...
Abstract The industrial yeast Komagataella phaffii (formerly named Pichia pastoris ) is commonly used to synthesize recombinant proteins, many of which are as human therapeutics or in food. However, the basic strain, NRRL Y-11430, from all commercial hosts derived, not available without restrictions on its use. Comparative genome sequencing leaves little doubt that Y-11430 derived a K. type strain deposited UC Davis Phaff Yeast Strain Collection 1954. We analysed four equivalent strains...
Lignocellulosic biomass is recalcitrant toward deconstruction into simple sugars due to the presence of lignin. To render lignocellulosic a suitable feedstock for bio-based economy, plants can be engineered have decreased amounts However, with lowest lignin exhibit collapsed vessels and yield penalties. Previous efforts were not able fully overcome this phenotype without settling in sugar upon saccharification. Here, we reintroduced CINNAMOYL-COENZYME A REDUCTASE1 (CCR1) expression...
Leaves are the plant's powerhouses, providing energy for all organs through sugar production during photosynthesis. However, sugars serve not only as a metabolic source sink tissues but also signaling molecules, affecting gene expression conserved pathways to regulate plant growth and development. Here, we describe an in vitro experimental assay, allowing one alter sucrose (Suc) availability early Arabidopsis (Arabidopsis thaliana) leaf development, with aim identify affected cellular...
Synchrotron radiation based nanoscopic X-ray fluorescence (SR nano-XRF) analysis can visualize trace level elemental distribution in a fully quantitative manner within single cells. However, in-air XRF requires chemical fixation modifying the cell's composition. Here, we describe first upon cryogenically frozen (-150°C) fibroblasts at ID16A-NI 'Nano-imaging' end-station located European Radiation Facility (ESRF) Grenoble (France). Fibroblast cells were obtained from skin biopsies control and...
In this work, the three-dimensional elemental distribution profile within freshwater crustacean Ceriodaphnia dubia was constructed at a spatial resolution down to 5 μm via data fusion approach employing state-of-the-art laser ablation-inductively coupled plasma-time-of-flight mass spectrometry (LA-ICP-TOFMS) and laboratory-based absorption microcomputed tomography (μ-CT). C. exposed elevated Cu, Ni, Zn concentrations, chemically fixed, dehydrated, stained, embedded, prior μ-CT analysis....
Streptococcus anginosus, Pseudomonas aeruginosa and Staphylococcus aureus are often co-isolated from the sputum of cystic fibrosis patients. It was recently shown that S. anginosus is protected activity vancomycin when it grows in a multispecies biofilm with P. aureus. Elucidating underlying cause reduced susceptibility to growing The transcriptome compared monospecies biofilm. Subsequently, transmission electron microscopy performed investigate changes cell wall morphology response growth...
Article11 September 2020Open Access Transparent process Zinc inhibits lethal inflammatory shock by preventing microbe-induced interferon signature in intestinal epithelium Jolien Souffriau Center for Inflammation Research, VIB, Ghent, Belgium Department of Biomedical Molecular Biology, Ghent University, Search more papers this author Steven Timmermans Tineke Vanderhaeghen Charlotte Wallaeys Kelly Van Looveren Lindsy Aelbrecht Sylviane Dewaele Vandewalle orcid.org/0000-0003-1844-6476 Evy...
In addition to the nucleus, mitochondria and chloroplasts in plant cells also contain genomes. Efficient DNA repair pathways are crucial these organelles fix damage resulting from endogenous exogenous factors. Plant organellar genomes complex compared with their animal counterparts, although several plant-specific mediators of organelle have been reported, many regulators remain be identified. Here, we show that a mitochondrial SWI/SNF (nucleosome remodeling) B protein, SWIB5, is capable...
Buckminsterfullerene (C60) has a large potential for biomedical applications. However, the main challenge realization of its application is to overcome extremely low water solubility. One approach coformulation with biocompatible water-soluble polymers, such as poly(2-oxazoline)s (PAOx), form C60 nanoparticles (NPs). uniform and defined NPs have only been obtained via thin film hydration method or using cyclodextrin-functionalized PAOx. Here, we report mechanochemical preparation stable...
The molecular divergence, morphology and pathology of a cryptic gregarine that is related to the bee parasite Apicystis bombi Lipa Triggiani, 1996 described. 18S ribosomal DNA gene sequence new was equally dissimilar A. closest genus Mattesia Naville, 1930, although phylogenetic analysis supported closer relation bombi. Pronounced divergence with found in ITS1 (69.6% similarity) seven protein-coding genes (nucleotide 78.05% protein 90.2% similarity). isolated from Bombus pascuorum Scopoli,...
In this study, transmission electron microscopy (TEM) and cryo-scanning (cryo-SEM) were evaluated for their ability to detect lipid bodies in microalgae. To do so, Phaeodactylum tricornutum Nannochloropsis oculata cells harvested both the mid-exponential early stationary growth phase. Two different cryo-SEM cutting methods compared: cryo-planing freeze-fracturing. The results showed that, despite longer preparation time, TEM visualisation preceded by cryo-immobilisation allows a clear...
Abstract The structural organization of the Golgi stacks in mammalian cells is intrinsically linked to function, including glycosylation, but role morphology less clear lower eukaryotes. Here we investigated link between and secretory pathway function using Pichia pastoris as a model system. To unstack cisternae, disrupted 18 genes encoding proteins without loss viability. Using biosensors, confocal microscopy transmission electron identified three strains with irreversible perturbations...
Abstract In the standard toolkit for recombinant protein expression, yeast known in biotechnology as Pichia pastoris (formally: Komagataella phaffii ) takes up position between E. coli and HEK293 or CHO mammalian cells, is used by thousands of laboratories both academia industry. The organism eukaryotic yet microbial, grows to extremely high cell densities while secreting proteins into its fully defined growth medium, using very well established strong inducible constitutive promoters. Many...