A5114548698- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- Advanced biosensing and bioanalysis techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Protein Degradation and Inhibitors
- RNA and protein synthesis mechanisms
ETH Zurich
2025
University of Zurich
2024
Abstract The metazoan tRNA ligase complex (tRNA-LC) has essential roles in biogenesis and unfolded protein response. Its catalytic subunit RTCB contains a conserved active-site cysteine that is susceptible to metal ion-induced oxidative inactivation. flavin-containing oxidoreductase PYROXD1 preserves the activity of human tRNA-LC NAD(P)H-dependent manner, but its protective mechanism remains elusive. Here, we report cryogenic electron microscopic structure RTCB–PYROXD1 complex, revealing...
We have developed a method combining microinjection and automated fluorescence microscopy to continuously assess the degradation rate, subcellular localization intracellular concentration of protein analytes at single-cell level. Cells are unperturbed grown in unaltered environmental conditions show high viability. The injection defined ratios concentrations allows for clearly starting point degradation, without entanglement biosynthesis/uptake, often encountered existing methods....