A5103160522- Immune Cell Function and Interaction
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Diabetes and associated disorders
- Immune Response and Inflammation
- Nanowire Synthesis and Applications
- Lung Cancer Research Studies
- Silicon Carbide Semiconductor Technologies
- Monoclonal and Polyclonal Antibodies Research
- Protein Degradation and Inhibitors
- Pancreatic function and diabetes
- Trypanosoma species research and implications
- Viral Infectious Diseases and Gene Expression in Insects
- Advancements in Semiconductor Devices and Circuit Design
- Immunodeficiency and Autoimmune Disorders
- Cell Adhesion Molecules Research
- Enterobacteriaceae and Cronobacter Research
- Calcium signaling and nucleotide metabolism
- Diabetes Management and Research
- Glycosylation and Glycoproteins Research
- Virus-based gene therapy research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Phagocytosis and Immune Regulation
- Liver physiology and pathology
Cellectis (United States)
2021-2024
Columbia University Irving Medical Center
2016-2023
New York Proton Center
2020
Universidad de Cantabria
2009-2016
Instituto de Investigación Marqués de Valdecilla
2015-2016
Marqués de Valdecilla University Hospital
2015
Institut de Recherche pour le Développement
2011
University of Gothenburg
2008
Universal CAR T-cell therapies are poised to revolutionize cancer treatment and improve patient outcomes. However, realizing these advantages in an allogeneic setting requires universal T-cells that can kill target tumor cells, avoid depletion by the host immune system, proliferate without attacking tissues. Here, we describe development of a novel immune-evasive scaffold using precise TALEN-mediated gene editing DNA matrices vectorized recombinant adeno-associated virus 6. We simultaneously...
Patients with nonalcoholic steatohepatitis (NASH) have increased expression of liver monocyte chemoattractant protein-1 (MCP-1), but its cellular source and contribution to various aspects NASH pathophysiology remain debated. We demonstrated CCL2 (which encodes MCP-1) in patients NASH, commensurately, a 100-fold increase hepatocyte Ccl2 mouse model accompanied by monocyte-derived macrophage (MoMF) infiltrate fibrosis. To test repercussions hepatocyte-derived MCP-1, we generated...
Adoptive cell therapy using chimeric antigen receptor (CAR) T cells has proven to be lifesaving for many cancer patients. However, its therapeutic efficacy been limited in solid tumors. One key factor this is cancer-associated fibroblasts (CAFs) that modulate the tumor microenvironment (TME) inhibit infiltration and induce "T dysfunction." Additionally, sparsity of tumor-specific antigens (TSA) expression CAR-directed tumor-associated (TAA) on normal tissues often results "on-target...
Dendritic cells (DC) express a functional NADPH oxidase and produce reactive oxygen species (ROS) upon interaction with microbes T cells. Exposure to ROS leads DC activation maturation, as evidenced by phenotypic changes. We have evaluated how endogenous production affects the cytokine secretion pattern cell-activating capacity of bone marrow-derived murine DC. treated scavengers, well from mice that lack (and thereby inherently deficient in production) produced significantly increased...
Thiazolidinediones (TZDs) are PPARγ agonists with potent insulin-sensitizing effects. However, their use has been curtailed by substantial adverse effects on weight, bone, heart, and hemodynamic balance. TZDs induce the deacetylation of K268 K293 to cause browning white adipocytes. Here, we show that targeted mutations resulting in constitutive (K268R/K293R, 2KR) increased energy expenditure protected from visceral adiposity diet-induced obesity augmenting brown remodeling adipose tissues....
CD38, a type II transmembrane glycoprotein expressed in many cells of the immune system, is involved cell signaling, migration and differentiation. Studies CD38 deficient mice (CD38 KO mice) indicate that this molecule controls inflammatory responses, although its involvement these responses depends on disease model analyzed. Here, we explored role control autoimmune using chicken collagen (col II) immunized C57BL/6-CD38 as collagen-induced arthritis (CIA). We demonstrate develop an...
Abstract Objective To explore the bidirectional relationship between development of rheumatoid arthritis (RA) and atherosclerosis using bovine type II collagen (CII)–immunized B10.RIII apoE −/− mice, a murine model spontaneous collagen‐induced (CIA). Methods Male mice wild‐type controls were immunized with 150 μg CII emulsified in Freund's complete adjuvant (CFA). The clinical, radiologic, histopathologic severity CIA, levels circulating IgG1 IgG2a anti‐CII antibodies, expression...
Summary Objective To demonstrate the feasibility of a house‐to‐house screening system used for congenital Chagas disease in rural areas based on an active search pregnant women and newborns their homes addition to passive case detection health facilities. Methods Exploratory phase conducted by research team followed operational period coordinated municipal service. A blood sample was taken serological parasitological tests Trypanosoma cruzi from who were searching antenatal care or visited...
CD5 is a lymphoid-specific transmembrane glycoprotein constitutively expressed on thymocytes and mature T B1a lymphocytes. Current data support the view that negative regulator of antigen-specific receptor-mediated signaling in these cells, this would likely be achieved through interaction with ligand/s (CD5L) still undefined nature immune or accessory cells. To determine functional consequence loss CD5/CD5L vivo, new transgenic mouse line was generated (shCD5EμTg), expressing circulating...
Objective Transforming growth factor β (TGFβ) plays a prominent role in the establishment of immunologic tolerance, and mice lacking TGFβ1 die multiorgan inflammation early life. TGFβ controls differentiation CD4+ lymphocytes into Treg cells or proinflammatory Th17 cells. Although this dual capacity is modulated by presence additional cytokines around activated cells, also dissociates Th17/Treg cell dose‐dependent manner mechanisms still unknown. The purpose study was to explore contribution...
Abstract Despite the remarkable success of autologous chimeric antigen receptor (CAR) T cells, some patients relapse due to tumor escape and low or uneven expression, among other mechanisms. Therapeutic options after are limited, emphasizing need optimize current approaches. In addition, there is a develop allogeneic “off-the-shelf” therapies from healthy donors that readily available at time treatment decision can overcome limitations To address both challenges simultaneously, we generated...
Abstract Escherichia coli heat‐labile enterotoxin (LT) exhibits a broad range of immunomodulatory activities, including the induction lymphocyte‐programmed cell death. In previous studies, we have demonstrated that in vivo LT promotes apoptosis immature T and B cells through stimulation endogenous glucocorticoids. present study, show extrinsic cell‐death pathway as well apoptosis‐inducing factor do not participate LT‐induced elimination thymocytes. contrast to developing lymphocytes, death...
Despite the importance of Treg cells in maintenance immunologic tolerance, mechanisms that control their generation and activity are unknown. Since cell cycle inhibitor p27(Kip1) (p27) was involved T anergy, we undertook this study to explore its role both processes.The development type II collagen-induced arthritis (CIA) lupus-like abnormalities compared between transgenic mice overexpressing human Bcl-2 (BCL2-TgT mice) nontransgenic were deficient or not p27. The contribution disease...
The inhibition of apoptotic cell death in T cells through the dysregulated expression BCL2 family members has been associated with protection against development different autoimmune diseases. However, multiple mechanisms were proposed to be responsible for such protective effect. purpose this study was explore effect T-cell overexpression BCL2A1, an anti-apoptotic member without on cycle progression, collagen-induced arthritis. Our results demonstrated attenuated arthritis these transgenic...
Antigen-specific immunotherapy of type 1 diabetes, typically via delivery a single native β cell antigen, has had little clinical benefit to date. With increasing evidence that diabetogenic T cells react against multiple antigens, including previously unappreciated neo-antigens can be emulated by mimotopes, shift from protein- epitope-based therapy is warranted. To this end, we aimed achieve efficient co-presentation major epitopes targeting both CD4+ and CD8+ cells. We have compared versus...
Significance Antigen-specific immunotherapy may be improved by focusing on epitopes that are disease-relevant and known to presented an individual’s human leukocyte antigen (HLA) haplotype, while targeting T cells across multiple antigens including specific neoepitopes not present in protein and/or produced beyond inflamed sites. Here, we provide proof of principle such a strategy applied tolerogenic DNA vaccination is effective preclinical model autoimmune diabetes, paving the way for...
To determine the role of pregnancy on Trypanosoma cruzi parasitemia, a matched cohort study was carried out in rural Bolivian community comparing parasite rates gravidae, puerperae, and non-pregnant infected women. A selection 67 chronically women, who delivered between March 2004 May 2005, were initially evaluated during third trimester again after delivery. They for age, parity, location with 104 seropositive likewise had submitted blood microscopic examination T. parasites June 2005....
Collagen‐type‐II‐induced arthritis (CIA) is an autoimmune disease, which involves a complex host systemic response including inflammatory and reactions. CIA milder in CD38 −/− than wild‐type (WT) mice. ProteoMiner‐equalized serum samples were subjected to 2D‐DiGE MS‐MALDI‐TOF/TOF analyses identify proteins that changed their relative abundances versus WT mice either with (CIA + ), no − or inflammation (complete Freund's adjuvant (CFA)‐treated mice). Multivariate revealed multiprotein...
The Escherichia coli heat-labile enterotoxin (LT) possesses a powerful mucosal and systemic adjuvant effect. However, little is known about the cellular molecular basis of immunostimulatory activity LT at level, even less information available on mechanisms underlying its activity. In this study, we show that distinct are responsible for parenteral adjuvanticity LT. Indeed, administration upregulates expression glucocorticoid-induced TNFR-related protein (GITR), but not other activation...
This data article presents the results of all statistical analyses applied to relative intensities detected 2D-DiGE protein spots for each 3 performed DiGE experiments. The reveals specific subsets with significant differences between WT and CD38-deficient mice either Collagen-induced arthritis (CIA), or chronic inflammation induced by CFA, under steady-state conditions. also shows MS that allowed identification species which serve discriminate different experimental groups used in this...