- Particle physics theoretical and experimental studies
- Quantum Chromodynamics and Particle Interactions
- Reproductive Biology and Fertility
- Pluripotent Stem Cells Research
- Renal and related cancers
- High-Energy Particle Collisions Research
- Animal Genetics and Reproduction
- Nuclear physics research studies
- CRISPR and Genetic Engineering
- Medical Imaging Techniques and Applications
- Atomic and Subatomic Physics Research
- Particle accelerators and beam dynamics
- Superconducting Materials and Applications
- Neutrino Physics Research
- Particle Accelerators and Free-Electron Lasers
- Anaerobic Digestion and Biogas Production
- Telomeres, Telomerase, and Senescence
- Dark Matter and Cosmic Phenomena
- Microtubule and mitosis dynamics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Advanced machining processes and optimization
- Biofuel production and bioconversion
- Muon and positron interactions and applications
- Soft Robotics and Applications
- Prenatal Screening and Diagnostics
China University of Mining and Technology
2023
University of Connecticut
2002-2013
Reproductive Biology Associates
2006-2011
Peking University
2005-2007
Abstract The effects of the electrical field strengths, number pulses, and post‐activation media on chromatin conformation parthenogenetic development were studied to optimize activation protocol for porcine nuclear transfer. In experiment 1, strengths examined. Oocytes subjected square direct current pulses at output voltages 1.2, 1.7, 2.2, 2.7 kV/cm 1 × 30 μsec. voltage resulting from was 2.2 kV/cm, in which 50.0% activated oocytes developed blastocysts vitro. 2, influence 3 blastocyst...
Swamp buffalos are becoming endangered due to reproductive inefficiencies. This is of concern because many countries depend heavily on their products. Somatic cell nuclear transfer (SCNT) a potential strategy for preserving species. To date, SCNT in swamp buffalo has succeeded the creation blastocyst embryos. However, development term extremely limited, and only 1 live birth been reported. An abnormal epigenetic mechanism suspected be cause developmental failure, as also seen other The DNA...
To determine if the development of somatotropic axis in somatic clones (clones) is similar to that heifers produced by artificial insemination (controls), serum samples were collected every 30 min for 6 h, once per month, 7 mo from 4 generated a 13-yr-old cow and age-matched controls. Average concentrations growth hormone (GH) not different between controls, GH declined over time insulin-like factor I (IGF-I) less than IGF-I increased both groups. Concentrations IGF-binding protein 3...
Multiple methods exist that can reprogram differentiated cells to a pluripotent state similar of embryonic stem (ESCs). These include somatic cell nuclear transfer (SCNT), fusion-mediated reprogramming (FMR) with ESCs, and the production induced (iPSCs). All these yield in which endogenous Oct4 gene is reactivated. We were interested comparing activity promoter three different classes cells, including normal FMR (FMRCs), iPSCs. prepared all types harbor transgene composed mouse driving green...
Although leukemia inhibitory factor (LIF) maintains the ground state pluripotency of mouse embryonic stem cells and induced pluripotent (iPSC) by activating Janus kinase/signal transducer activator transcription 3 (Jak/Stat3) pathway, mechanism remains unclear. Stat3 has only been shown to promote complete reprogramming epiblast neural cells, partially reprogrammed (pre-iPSC). We investigated if how Jak/Stat3 activation promotes terminally differentiated fibroblasts (MEF). demonstrated that...
Abstract To solve the constant contact force control problem between end tool of a 5 degrees freedom hybrid optical mirror processing robot and workpiece, an adaptive impedance method for pneumatic servo-polishing system is designed. Firstly, at set up. Secondly, investigated based on mathematical model system. Additionally, causes steady-state error are analyzed theoretically, calculation deduced. Finally, indirect controller Lyapunov Stability Principle developed to estimate environmental...
Phenotypic abnormalities in somatic cell nuclear transfer (SCNT)-derived animals may be caused by incomplete epigenetic reprogramming of donor cells. We previously observed an association between delayed alveologenesis and dysregulated gene expression the lungs SCNT-derived pigs. Since DNA methylation is one major modifications genome that regulate expression, we compared global loci-specific cytosine deceased neonatal cloned pigs (n=5) one-month old live to those age-matched controls (n=5...