A5056467420- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Single-cell and spatial transcriptomics
- Renal and related cancers
- Genetics, Bioinformatics, and Biomedical Research
- Scientific Computing and Data Management
- Animal Genetics and Reproduction
- Hedgehog Signaling Pathway Studies
- Genetic diversity and population structure
- Biomedical Text Mining and Ontologies
- Genetic and phenotypic traits in livestock
- Wnt/β-catenin signaling in development and cancer
- Epigenetics and DNA Methylation
- Biomedical and Engineering Education
- Fish Ecology and Management Studies
- Genomics and Chromatin Dynamics
- Developmental Biology and Gene Regulation
Michigan State University
2016-2024
University of California, Santa Cruz
2013-2014
Trent University
2009
University of Wisconsin–Madison
2005
Pioneer transcription factors (TFs), such as OCT4 and SOX2, play crucial roles in pluripotency regulation. However, the master TF-governed regulatory circuitry was largely inferred from cultured cells. In this work, we investigated SOX2 binding embryonic day 3.5 (E3.5) to E7.5 mouse. E3.5 inner cell mass (ICM), regulates ICM-trophectoderm program but is dispensable for opening global enhancers. Instead, occupies preaccessible enhancers part opened by early-stage expressing TFs TFAP2C NR5A2....
The reprogramming factors OCT4, SOX2, KLF4, and MYC (OSKM) can reactivate the pluripotency network in terminally differentiated cells, but also regulate expression of non-pluripotency genes other contexts, such as mouse primitive endoderm. endoderm is an extraembryonic lineage established parallel to pluripotent epiblast blastocyst, progenitor pool for stem (XEN) cells. We show that OSKM induce endodermal genes, leading formation induced XEN (iXEN) which possess key properties...
Abstract An unintended consequence of fish stocking is genetic homogenization from interbreeding between indigenous populations and genetically distinct hatchery strains. Lake trout in southern Ontario have been extensively supplemented with strains originating Great Lakes sources, but evaluation introgressive admixture has challenging without data or samples that precede historical events. We used complementary markers (mitochondrial PCR‐RFLP 12 microsatellite DNA loci) to resolve native...
Summary During development, progenitors of embryonic stem (ES) and extraembryonic endoderm (XEN) cells are concomitantly specified within the inner cell mass (ICM) mouse blastocyst. Similarly, XEN induced (iXEN cells) alongside pluripotent (iPS) following overexpression Oct4 , Sox2 Klf4 Myc (OSKM) during somatic reprogramming. It is unclear how or why this cocktail produces both types, but OCT4 has been associated with non-pluripotent outcomes. In report, we show that, OSKM reprogramming,...
Abstract Learning to write a scientific manuscript is one of the most important and rewarding training experiences, yet young scientists only embark on this experience relatively late in graduate school, after gathering sufficient data lab. Yet, familiarity with process writing receiving peer reviews, often leads more focused driven experimental approach. To jump-start training, we developed protocol for teaching reviewing classroom, appropriate new or upper-level undergraduate students...
Learning to write a scientific manuscript is one of the most important and rewarding training experiences, yet young scientists only embark on this experience relatively late in graduate school after gathering sufficient data lab. Familiarity with process writing receiving peer reviews often leads more focused driven experimental approach as well better understanding literature. To jump-start training, we developed protocol for teaching reviewing course, appropriate new or upper-level...