We established a necrotizing enterocolitis (NEC) rat model and explored the role of bifidobacteria in intestines rats its regulation on intestinal Toll-like receptors (TLRs). Seventy-five newborn Sprague-Dawley were randomly divided into 5 groups (15 rats/group): group A, artificial feeding (formula-fed); B, NEC (LPS + formula-fed); C, bifidobacterium formula-fed microcapsules, intragastric administration); D, (formula-fed microcapsules gavage); E, breast-feeding (rat breast-feeding). After...

The aim of the present study was to investigate association neonatal necrotizing enterocolitis (NEC) with myeloid differentiation‑(MD‑2) and GM2 activator protein (GM2A) genetic polymorphisms. Gene resequencing MD‑2 GM2A gene exons performed on 42 neonates, diagnosed NEC (NEC group), as well in rs11465996 locus, located promoter region. detect polymorphisms neonates compare functional polymorphic loci 83 without (control who had been born during same period. A locus abnormal frequency...

Prader-Willi syndrome is a rare genetic abnormality that can be challenging to diagnose early, but for which early interventions improve prognosis. To understanding of Prader–Willi in neonates Asia, we retrospectively analyzed the clinical records 20 affected newborns diagnosed Department Neonatology, Guangzhou Women and Children's Medical Center, Guangzhou, China from January 2007 December 2014 performed review relevant literature. Fourteen boys six girls presented with hypotonia, poor...

We studied the demographic and clinical characteristic, risk factors, outcomes of full-term small-for-gestational-age (SGA) infants born to mothers with gestational diabetes mellitus (GDM) in China. A retrospective case-control study that included 1981 SGA was conducted; data between without GDM were compared. Of 383 GDM, 221 (57.7%) female, incidence these 1 155 live births. The siblings (RR, 1.88; 95% CI, [1.23-2.86]), low 1- 5-min Apgar scores (RR,2.04 4.21; 95%CI [1.05-4.00]...

Early alterations in glucose homeostasis increase the risk of developing insulin resistance (IR) and obesity later life. The study aimed to ascertain peripheral blood levels hormones that controlling inflammatory factors are correlated with IR fetal outcomes small-for-gestational-age (SGA) infants born mothers gestational diabetes mellitus (GDM).This cohort included a total 90 SGA GDM (n = 37) without 53). At birth, glucose, insulin, C-peptide, growth hormone (GH), IGFBP3, lipid profiles,...

We investigated the effect of inactivated Bifidobacterium on mRNA expression TRAF6, GSK-3β, and microRNA-146a in lipopolysaccharide (LPS)-stimulated rat small intestinal epithelial cells (IEC-6s). IEC-6s were randomly divided into an LPS group, a culture supernatant bacteria group. After stimulation with for 5 h, three groups treated as follows: group was cultured 24 h sterile saline; (infantis strain) h; infantis h. Reverse transcription polymerase chain reaction used to determine levels....

Objective To study the effect of bifidobacterium on intestinal tissue necrotizing enterocolitis (NEC) in newborn rats and its regulation Wnt/β-Catenin signal pathway. Methods Seventy-five SD were randomly divided into 5 groups, each group had 15 rats.Group A was artificial feeding control group; B NEC model C treatment D feeding+ E rat breast group.The localization expression Toll-like receptor 4(TLR4) ileocecal ileum detected by immunohistochemical detection, also equivalen-tileum...

Objective To discuss the possible molecular mechanisms involved in protective effects of Bifidobacterium on intestinal tissue necrotizing enterocolitis (NEC) newborn rats. Methods Seventy-five Sprague-Dawley rats (born within 2 h) were randomly divided into five groups, each group with 15 rats. Group A was NEC model group, and fed lipopolysaccharide (LPS) formula. B treatment LPS formula micro-capsule. C artificial feeding control D E breastfeeding rat breast milk by mothers. 30 mg/kg...

Objective To investigate the effects of lipopolysaccharides(LPS) in different concentrations on proliferation and interleukin(IL)-6, IL-1β tumor necrosis factor-α(TNF-α) secretion intestinal epithelial cells(IEC-6) rats vitro. Methods IEC-6 were divided into normal group(0 mg/L, group A), 0.1 mg/L group(group B), 0.5 C), 1.0 D), 5.0 E) 10.0 F). Different groups cells exposed to LPS with for 3 h, 5 h 7 h. Thiazolyl blue(MTT)was performed IEC-6.The IL-6, TNF-α culture supernatant...

Objective To detect the effects of bifidobacterium or cultured supernatant on mRNA expression tumor necrosis factor receptor-associated 6(TRAF6), glycogen synthase kinase-3β(GSK-3β) and miRNA-146a in rat small intestinal epithelial cell(IEC-6) induced by lipopolysaccharide(LPS). Methods IEC-6 logarithmic phase were randomly divided into LPS group, group inactivated bacteria group.All 3 groups exposed to 5 mg/L for hours, then 1 mL sterile saline was added culturing continued 24 hours;...

Objective To study the glycomacropeptide (GMP) content of human milk in different puerperal stages parts China, and to provide evidence optimize component artificial formula. Methods Five hundred ninety-four cases puerperas from Guangzhou, Shanghai Jingjiang regions were selected divided into 18 groups by regions, gestational age (preterm, full term) ages (colostrum, transitional mature milk). Five mL front was collected each puerpera.The processed rennet enzyme other steps, then...

Objective To investigate the relationship between myeloid differentiation (MD-2) gene polymorphisms and necrotizing enterocolitis (NEC) in neonates.Methods A gene-sequencing method was used to re-sequence exons promoter functional polymorphism region (rs11465996) of MD-2 42 NEC neonates admitted neonatal intensive care unit Women Children's Medical Center,Guangzhou University from June 1,2011 May 31,2012.These loci were compared with 83 non cases.The Chi square test for statistical...

Objective To explore the effects of glycomacropeptide (GMP) in human milk and formula on proliferation bifidobacterium infantis their dose-response relationship. Methods Casein was isolated from 30 healthy postpartum women Guangzhou Women Children's Medical Center September 2014, hydrolyzed by rennet to obtain GMP, which then purified ultrafiltration ion exchange chromatography. Human GMP cow (0, 250, 500, 1 000,1 2 000 3 mg/L) were added liquid medium, cultured under anaerobic...

Objective To investigate the effect of heparin-binding epidermal growth factor-like factor (HB-EGF) on mitochondrial pathway apoptosis in rats with neonatal necrotizing enterocolitis (NEC).Methods Sprague-Dawley were randomly divided into three groups ten each.NEC group formula fed,and hypoxia exposed by 100％ N2 for 90 s and cold stress at 4 ℃ 10 min twice a day days.Additionally,rats HB-EGF received 800μg/kg gavage four times days.Rats control given breast milk feeding days without any...

Objective To detect the glycomacropeptide(GMP) content of human breast milk, and take it as reference newborn infant formula milk powder to optimize nutritional formulas. Methods Thirty primiparas who fit following conditions were selected: healthy, no special diet habits, living stably, having adequate aged between 25 39 years old, term delivery, selected.Then they divided into 2 groups, colostrum group mature group, each had 15 cases.Each case been collected 5 mL(front milk). The...