A5092424848- Advanced Fluorescence Microscopy Techniques
- Optical Coherence Tomography Applications
- Liver Disease Diagnosis and Treatment
- Cell Image Analysis Techniques
- Digital Holography and Microscopy
- Drug-Induced Hepatotoxicity and Protection
- Advanced Biosensing Techniques and Applications
- Biochemical effects in animals
- Medical Imaging Techniques and Applications
- Photodynamic Therapy Research Studies
- Eicosanoids and Hypertension Pharmacology
- Photoacoustic and Ultrasonic Imaging
- Apelin-related biomedical research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Kruppel-like factors research
- Optical Imaging and Spectroscopy Techniques
- Near-Field Optical Microscopy
- Advanced Optical Sensing Technologies
Bielefeld University
2022-2025
Super-resolved structured illumination microscopy (SR-SIM) is among the most flexible, fast, and least perturbing fluorescence techniques capable of surpassing optical diffraction limit. Current custom-built instruments are easily able to deliver two-fold resolution enhancement at video-rate frame rates, but cost still relatively high, physical size based on implementation their optics rather large. Here, we present our latest results towards realizing a new generation compact,...
Background: Reactive oxygen species (ROS) are prevalent in the liver during intoxication, infection, inflammation, and aging. Changes sinusoidal endothelial cells (LSEC) associated with various diseases. Methods: Isolated rat LSEC were studied under oxidative stress induced by H 2 O at different concentrations (0.5–1000 µM) exposure times (10–120 min). functions tested a dose-dependent time-dependent manner. Results: (1) Cell viability, reducing potential, scavenging function decreased as...
Abstract Super-resolved structured illumination microscopy (SR-SIM) is among the most flexible, fast, and least perturbing fluorescence techniques capable of surpassing optical diffraction limit. Current custom-built instruments are easily able to deliver two-fold resolution enhancement at video-rate frame rates, but cost still relatively high, physical size based on implementation their optics rather large. Here, we present our latest results towards realizing a new generation compact,...
Abstract Reactive oxygen species (ROS) are prevalent in the liver during intoxication, infection, inflammation, and ageing. Changes sinusoidal endothelial cells (LSECs) associated with various diseases. We investigated how oxidative stress induced by H 2 O affects isolated rat LSECs at different concentrations (0.5-1000µM) exposure times (10-120 min). Our findings show that several LSEC functions a dose- time-dependent manner: (1) cell viability, reducing potential, scavenging function...
Abstract Atherogenesis is associated with elevated plasma levels of oxidized low-density lipoproteins (oxLDL). In vivo, oxLDL causes liver endothelial swelling, and disrupts sinusoidal cell (LSECs) fenestrations. We mapped the nanoscale kinetics these changes in vitro isolated rat LSECs challenged monitored viability endocytosis cytotoxicity assays. OxLDL disrupted LSEC ultrastructure – increasing concentrations oxidation caused sieve plate loss, fenestration fusion, gap formation....
In super-resolution structured illumination microscopy (SR-SIM) the separation between opposing laser spots in back focal plane of objective lens affects pattern periodicity, and, thus, resulting spatial resolution. Here, we introduce a novel hexagonal prism telescope which allows us to seamlessly change parallel beams for 3 pairs beams, simultaneously. Each end is composed 6 Littrow prisms, are custom-ground so they can be grouped together form tight hexagon. By changing distance hexagons,...
In super-resolution structured illumination microscopy (SR-SIM) the separation between opposing laser spots in back focal plane of objective lens affects pattern periodicity, and, thus, resulting spatial resolution. Here, we introduce a novel hexagonal prism telescope which allows us to seamlessly change parallel beams for 3 pairs beams, simultaneously. Each end is composed 6 Littrow prisms, are custom-ground so they can be grouped together form tight hexagon. By changing distance hexagons,...
In super-resolution structured illumination microscopy (SR-SIM) the separation between opposing laser spots in back focal plane of objective lens affects pattern periodicity, and, thus, resulting spatial resolution. Here, we introduce a novel hexagonal prism telescope which allows us to seamlessly change parallel beams for 3 pairs beams, simultaneously. Each end is composed 6 Littrow prisms, are custom-ground so they can be grouped together form tight hexagon. By changing distance hexagons,...
Super-resolved structured illumination microscopy (SR-SIM) is among the most flexible, fastest and least perturbing fluorescence techniques capable of surpassing optical diffraction limit. Current custom-built instruments are easily able to deliver two-fold resolution enhancement at video-rate frame rates, but cost still relatively high physical size prohibitively large. We have developed a compact, cost-efficient high-speed fiber-based 2D-, grazing-incidence-, TIRF 3D-SIM overcome these...
Optical projection tomography (OPT) is a 3D imaging technique developed by Sharpe et al. [1] in 2002, which used for non-invasive of biological samples. Typical sample sizes lie the order several millimeters. Analogous to X-ray computed tomography, rotated, and 2D projections are obtained at different rotation angles. A reconstruction algorithm then reconstruct image from projections. Image contrast based on transmitted light, absorption, or types immunofluorescence-labeled structures...
Abstract Photonic waveguide chips offer near-field excitation of biological samples, which enables cost-effective, large field-of-view super-resolution microscopy without the need for high numerical aperture (NA) objective lenses. Single molecule localization based that requires illumination intensities is currently limited to solid state photonic composed hard-coated, NA planar waveguides deposited on opaque substrates. These platforms do not permit epi-detection fluorescence through...