A5095932210- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- Advanced Nanomaterials in Catalysis
- Nanocluster Synthesis and Applications
Albany State University
2024-2025
University at Albany, State University of New York
2024-2025
Salmonella, the most prevalent food-borne pathogen, poses significant medical and economic threats. Swift accurate on-site identification serotyping of Salmonella is crucial to curb its spread contamination. Here, a synthetic biology cascade reaction presented on paper substrate using CRISPR-Cas12a recombinase polymerase amplification (RPA), enabling programming standard toehold RNA switch for genome choice. This approach employs just one design differentiate between two different serotypes,...
Abstract The development of a paper‐based genome detection assay using target‐responsive DNA‐templated silver nanoclusters (DFN‐1) is presented. reported exhibit intrinsic fluorescence, which regulated by the cleavage DNA template surrounding core. To enable to respond specific genome, CRISPR‐Cas12a employed for highly and programmable digestion nanoclusters. Upon target, cleaved complex, leading reduction in fluorescence. This successfully demonstrates Salmonella liquid phase on 2 mm solid...