A5109876796- Photosynthetic Processes and Mechanisms
- Enzyme Structure and Function
- Enzyme Catalysis and Immobilization
- Protein Structure and Dynamics
- Pancreatic function and diabetes
- Hemoglobin structure and function
- Biochemical and Molecular Research
- Metabolism and Genetic Disorders
- Chemical Synthesis and Analysis
- Healthcare Systems and Technology
- Protein Interaction Studies and Fluorescence Analysis
- Analytical Chemistry and Chromatography
- Biotin and Related Studies
- Metabolomics and Mass Spectrometry Studies
- Peptidase Inhibition and Analysis
- Glycosylation and Glycoproteins Research
- Computational Drug Discovery Methods
- Asian Industrial and Economic Development
- Mass Spectrometry Techniques and Applications
- Erythrocyte Function and Pathophysiology
- Protein purification and stability
- Cancer, Hypoxia, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- ATP Synthase and ATPases Research
- Amazonian Archaeology and Ethnohistory
University of Glasgow
2024
Adam Smith Institute
2024
Institute of Biological Chemistry, Academia Sinica
2024
National Taiwan University
2024
Institute of Biophysics
1987-1998
University of California, Davis
1995
Laboratoire des Biomolécules
1994
Academia Sinica
1955-1992
John Cochran VA Medical Center
1991
Mallinckrodt (United States)
1991
D-Glyceraldehyde-3-phosphate dehydrogenase (GAP-DH) is a protein containing no disulfide bonds; the guanidine HCl-denatured enzyme shows only limited extent of refolding and reactivation upon dilution, particularly prone to aggregation during dilution process. With increasing GAPDH concentration, decreases increases. The presence isomerase in mixture markedly increases at same time prevents as shown by light-scattering measurements. It suggested that denatured faced with two competing...
Research Article| August 01 1951 Cytochrome c modified by digestion with proteolytic enzymes: 1. Digestion C. L. Tsou 1Molteno Institute, University of Cambridge Search for other works this author on: This Site PubMed Google Scholar Biochem J (1951) 49 (3): 362–367. https://doi.org/10.1042/bj0490362 Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Facebook Twitter LinkedIn MailTo Cite Get Permissions Citation Tsou; Digestion. 1 1951; doi: Download...
Research Article| February 01 1952 Exogenous and endogenous cytochrome c C. L. Tsou 1Molteno Institute, University of Cambridge Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1952) 50 (4): 493–499. https://doi.org/10.1042/bj0500493 Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Facebook Twitter LinkedIn MailTo Cite Get Permissions Citation Tsou; c. 1 1952; doi: Download citation file: Ris (Zotero) Reference...
Research Article| February 01 1952 Reaction between hydrocyanic acid, cyanide ion and ferricytochrome c P. George; George 1Department of Colloid Science, University Cambridge Molteno Institute, Search for other works by this author on: This Site PubMed Google Scholar C. L. Tsou Author article information Publisher: Portland Press Ltd © CAMBRIDGE UNIVERSITY PRESS1952 Biochem J (1952) 50 (4): 440–448. https://doi.org/10.1042/bj0500440 Views Icon Article contents Figures & tables Video Audio...
Research Article| September 01 1951 On the cyanide inactivation of succinic dehydrogenase and relation to cytochrome b C. L. Tsou 1Molteno Institute, University Cambridge Search for other works by this author on: This Site PubMed Google Scholar Biochem J (1951) 49 (4): 512–520. https://doi.org/10.1042/bj0490512 Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Facebook Twitter LinkedIn Email Cite Get Permissions Citation Tsou; b. 1 1951; doi:...
It has been previously reported that, during denaturation of creatine kinase by guanidinium chloride (GdmCl) or urea [Tsou (1986), Trends Biochem. Sci. 11, 427-429], inactivation occurs before noticeable conformational change can be detected, and it is suggested that the conformation at active site more easily perturbed hence flexible than molecule as a whole. In this study, thiol amino groups near are labelled with o-phthalaldehyde to form fluorescent probe. Both emission intensity...
Research Article| August 01 1951 Cytochrome c modified by digestion with proteolytic enzymes: 2. Properties of pepsin-modified cytochrome C. L. Tsou 1Molteno Institute, University Cambridge Search for other works this author on: This Site PubMed Google Scholar Biochem J (1951) 49 (3): 367–374. https://doi.org/10.1042/bj0490367 Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Facebook Twitter LinkedIn Email Cite Get Permissions Citation Tsou; c. 1...
Antigenic analysis using monoclonal antibodies and genomic ribonuclease protection was done on 47 isolates of group A respiratory syncytial virus (RSV) recovered from children in St. Louis during four RSV seasons. identified subgroups; the three that included more than one member, those designated A/2 A/2v had characteristic patterns. third subgroup, A/4, exhibited extensive heterogeneity, but all were distinguishable subgroups A/2v. Individual epidemic seasons representing multiple...
The 16-kD proteolipid subunit is the principal integral membrane protein of vaculor H+-ATPase (V-ATPase) complex that forms proton channel responsible for translocating protons across lipid bilayers. Two degenerate synthetic oligonucleotides, COT11 and COT12, corresponding to highly conserved transmembrane domains in all subunits sequenced so far, were used amplify a partial cDNA V-ATPase from cotton (Gossypium hirsutum L.) by polymerase chain reaction (PCR). These PCR products isolate two...
The inactivation and unfolding of lactate dehydrogenase (LDH) during denaturation by guanidinium chloride (GuHCl) under diverse conditions have been compared. Unfolding the native conformation, as monitored fluorescence c.d. measurements, occurs in two stages with increasing GuHCl concentrations, approximately coincides with, but slightly precedes, first stage unfolding. enzyme is inhibited to about 60-70% its original activity cross-linking glutaraldehyde or presence 1 M-(NH4)2SO4,...
The formation of native insulin either from scrambled or the separated A chain and B S-sulphonates by protein disulphide-isomerase was demonstrated with yields 20-30% as measured h.p.l.c. analysis, receptor binding stimulation lipogenesis. profile reaction products shows that, among all possible isomers containing both chains, hormone is far predominating product consequently most stable under certain conditions.
It has been shown previously [Tang, Wang & Tsou (1988) Biochem. J. 255, 451-455] that, under appropriate conditions, native insulin can be obtained from scrambled or the S-sulphonates of chains with a yield 25-30%, together reaction products containing separated A and B chains. The hormone is by far predominant product among isomers both now that presence added C peptide no appreciable effect on insulin. At higher temperatures content decreases whereas those increase, in case was absence...
The kinetics of reconstitution apoacylase with either Zn(II) or Co(II) and the inactivation reconstituted enzyme by 1,10-phenanthroline (OP) has been studied following substrate reaction continuously in presence metal ion OP respectively. Although native Zn(II)-containing Co(II)-reconstituted enzymes have closely similar Michaelis constants maximal velocities, for both apoenzyme ions differs considerably. For Co(II), show simple kinetics, but Zn(II), inhibition is a multi-phasic process...
The kinetic theory of the substrate reaction during modification enzyme activity previously described [Tsou (1988) Adv. Enzymol. Relat. Areas Mol. Biol. 61, 381-436] has been applied to a study on kinetics course inactivation aminoacylase by 1,10-phenanthroline. Upon dilution that had incubated with 1,10-phenanthroline into mixture, inhibited gradually increased, indicating dissociation reversible enzyme–1,10-phenanthroline complex. different concentrations chloroacetyl-L-alanine and...
Abstract This article investigates the rapid expansion of China’s new energy vehicle market in challenging context global economic uncertainties and pandemic, highlighting its significant growth since 2020. By delving deeper into sectoral geographical scope multi-level perspective concept, this study explores interactions among various sociotechnical systems that have accelerated development automotive electrification. The research offers a deep dive factors driving sector’s using...
Inactivation of pancreatic RNAase A occurs in guanidinium chloride (GdmCl) at low concentrations before the unfolding molecule as a whole can be detected [Liu and Tsou (1987) Biochim. Biophys. Acta 916, 455-464]. We have now shown that rate digestion by either trypsin or proteinase K increases significantly GdmCl where enzyme is largely inactivated, but fluorescence absorption measurements reveal no conformational changes. N-Terminal sequence analysis peptide fragments generated shows...
This study investigates how operational and dynamic capabilities have influenced the adaptation performance of Chinese automakers in response to industry shifts toward electrification intelligentisation from mid-2010s onward. Focusing on XPeng Motors, Jeep China, BYD, research examines varying levels, categorised as baseline, standard, VRIN (Valuable, Rare, Inimitable, Non-substitutable) resources, interact with shape strategic responses a rapidly evolving market. Using mixed-methods...
The following manuscript was published as a Fast Forward article on February 29, 2012: Sullivan TJ, Dairaghi DJ, Krasinski A, Miao Z, Wang Y, Zhao BN, Baumgart T, Berahovich R, Ertl LS, Pennell Seitz L, S, Ungashe Wei Johnson D, Boring Tsou C-L, Charo IF, Bekker P, Schall and Jaen JC, Characterization of CCX140-B, an orally bioavailable antagonist the CCR2 chemokine receptor, for treatment type 2 diabetes associated complications. J Pharmacol Exp Ther jpet.111.190918;...
Research Article| October 01 1951 The cytochrome system of adrenal medulla C. L. Tsou 1Molteno Institute, University Cambridge Search for other works by this author on: This Site PubMed Google Scholar Author and article information Publisher: Portland Press Ltd © CAMBRIDGE UNIVERSITY PRESS1951 Biochem J (1951) 49 (5): 658–662. https://doi.org/10.1042/bj0490658 Views Icon Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Facebook Twitter LinkedIn Email Cite...