A5016157046- DNA Repair Mechanisms
- Genomics and Chromatin Dynamics
- Virus-based gene therapy research
- Modular Robots and Swarm Intelligence
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Advanced Materials and Mechanics
- HIV Research and Treatment
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Micro and Nano Robotics
- Additive Manufacturing and 3D Printing Technologies
- RNA modifications and cancer
- Pluripotent Stem Cells Research
- Immune Cell Function and Interaction
- Optimization and Search Problems
Max Planck Institute for Multidisciplinary Sciences
2024
Friedrich Miescher Laboratory
2020-2021
Max Planck Society
2020-2021
Abstract The selection of an open reading frame (ORF) for translation eukaryotic mRNA relies on remodeling the scanning 48S initiation complex into elongation-ready 80S ribosome. Using cryo-electron microscopy, we visualize key commitment steps orchestrating in humans. Kozak sequence facilitates state and stabilizes closed by organizing contacts factors (eIFs) ribosomal proteins reconfiguring structure. GTPase-triggered large-scale fluctuations 48S-bound eIF2 facilitate eIF5B recruitment,...
In meiosis, DNA double-strand break (DSB) formation by Spo11 initiates recombination and enables chromosome segregation. Numerous factors are required for activity, couple the DSB machinery to development of a meiosis-specific 'axis-tethered loop' organisation. Through in vitro reconstitution budding yeast genetics, we here provide architectural insight into focussing on foundational factor, Mer2. We characterise interaction Mer2 with histone reader Spp1, show that directly associates...
The interferon-induced host cell protein Shiftless (SFL) inhibits −1 programmed ribosomal frameshifting (−1PRF) required for the expression of HIV-1 Gal-Pol and formation infectious particles. However, specific regions in SFL antiviral activity mechanism by which −1PRF remain unclear. Employing alanine scanning mutagenesis, we found that basic amino acids predicted zinc ribbon motif are essential suppression Gag-Pol but dispensable anti-HIV-1 activity. We have shown murine leukemia virus...
In meiosis, DNA double strand break (DSB) formation by Spo11 initiates recombination and enables chromosome segregation. Numerous factors are required for activity, couple the DSB machinery to development of a meiosis-specific ?axis-tethered loop? organization. Through in vitro reconstitution budding yeast genetics we here provide architectural insight into focussing on foundational factor, Mer2. We characterise interaction Mer2 with histone reader Spp1, show that directly associates...
This paper considers the shape formation problem within 3D hybrid model, where a single agent with strictly limited viewing range and computational capacity of deterministic finite automaton manipulates passive tiles through pick-up, movement, placement actions. The goal is to reconfigure set into specific termed an icicle. icicle, identified as dense, hole-free structure, strategically chosen function intermediate for more intricate tasks. It designed easy exploration by state agent,...
Motivated by the prospect of nano-robots that assist human physiological functions at nanoscale, we investigate coating problem in three-dimensional model for hybrid programmable matter. In this model, a single agent with strictly limited viewing range and computational capability deterministic finite automaton can act on passive tiles picking up tile, moving, placing it some spot. The goal is to fill each node surface graph size $n$ tile. We first solve restricted class graphs tile type,...