A5004963577- Parasitic Infections and Diagnostics
- Protein Hydrolysis and Bioactive Peptides
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Catalysis and Immobilization
- Chemical Synthesis and Analysis
- CRISPR and Genetic Engineering
- Biosensors and Analytical Detection
- Parasitic infections in humans and animals
- Advanced biosensing and bioanalysis techniques
- Antimicrobial Peptides and Activities
- Congenital Anomalies and Fetal Surgery
- Silk-based biomaterials and applications
- Collagen: Extraction and Characterization
- Biofuel production and bioconversion
- Plant biochemistry and biosynthesis
Jiangnan University
2020-2025
Suzhou Institute of Biomedical Engineering and Technology
2025
Chinese Academy of Sciences
2025
Bio-Medical Science (South Korea)
2025
Jiangsu Institute of Parasitic Diseases
2020
Acetaldehyde is a toxic pollutant that can be detoxified by acetaldehyde dehydrogenases (ADAs) through its conversion to acetyl-CoA. This study developed an integrated approach combining virtual screening, rational design, and dual scoring mechanism identify engineer hyperactive ADA variants. A library of 5000 Dickeya parazeae (DpADA) homologues was created protein BLAST, deep learning tools predicted their Kcat values. The top 100 candidates were selected based on binding affinity,...
We previously identified that the collagen mimetic peptide (CMP)-E3 is sufficient to induce in vitro blood clotting. In this study, we further demonstrated its ability vivo coagulation through a rat tail bleeding assay. However, intracellular expression of CMP-E3 negatively impacted Escherichia coli growth, causing cell density fluctuate within an optical at 600 nm (OD600) range 1.8-2.2, which limited high-level expression. To address challenge, developed programmed autolysis system by...
Collagen II (COL2) is the major component of cartilage tissue and widely applied in pharmaceuticals, food, cosmetics. In this study, COL fragments were extracted from human COL2 for secretory expression
Yarrowia lipolytica has been widely engineered as a eukaryotic cell factory to produce various important compounds. However, the difficulty of gene editing and lack efficient neutral sites make rewiring Y. metabolism challenging. Herein, Cas9 system was established redesign homologous recombination system, which caused more than 56-fold increase in HR efficiency. The fusion expression hBrex27 sequence C-terminus recruited Rad51 protein, decreased NHEJ, achieving 85% single-gene positive...
To establish a nucleic acid assay for detection of Echinococcus granulosus based on recombinase-aided isothermal amplification (RAA) assay.The 12S rRNA gene E. was selected as the target gene, and specific primers fluorescent probes RAA were designed, screened synthesized to granulosus. The sensitivity evaluated using different copy numbers sequence-contained recombinant plasmids various concentrations genomic DNA templates, specificity from granulosus, multilocularis, Schistosoma japonicum,...
To establish a novel nucleic acid assay for detection of Giardia lamblia based on the recombinase-aided isothermal amplification (RAA) assay, and evaluate its sensitivity specificity G. lamblia.The specific primer sequences florescent probes were designed synthesized β-giardin gene as target gene, fluorescent RAA was established. The recombinant plasmids at various copies (containing sequence) genomic DNA concentrations used templates to assess sensitivity, from lamblia, Schistosoma...