A5103248487- Hemoglobin structure and function
- Neonatal Health and Biochemistry
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Inflammatory Bowel Disease
- Microscopic Colitis
- Erythrocyte Function and Pathophysiology
- Clostridium difficile and Clostridium perfringens research
- Heme Oxygenase-1 and Carbon Monoxide
- Identification and Quantification in Food
- Enzyme Structure and Function
- Essential Oils and Antimicrobial Activity
- Protein Degradation and Inhibitors
- Food composition and properties
- Eosinophilic Esophagitis
- Toxic Organic Pollutants Impact
- Circular RNAs in diseases
- Developmental Biology and Gene Regulation
- Free Radicals and Antioxidants
- Extracellular vesicles in disease
- Multiple Myeloma Research and Treatments
- Industrial Technology and Control Systems
- Computational Drug Discovery Methods
- Ubiquitin and proteasome pathways
- Spectroscopy and Quantum Chemical Studies
Sun Yat-sen University
2020-2025
Sixth Affiliated Hospital of Sun Yat-sen University
2020-2025
Osaka University
2020-2024
Qingdao University
2023
Xiamen University
2022
Nanyang Technological University
2020
National Institute of Metrology
2017
Hong Kong Baptist University
2011-2013
Shanghai Jiao Tong University
2010-2013
This study investigated the formation of DNA adducts polybrominated diphenyl ethers (PBDEs) and possible mechanisms. adduction was conducted by in vitro reaction deoxyguanosine (dG) with PBDE-quinone (PBDE-Q) metabolites, were characterized using electrospray ionization tandem mass spectrometry. The results suggested involved Michael Addition between exocyclic NH(2) group at N-2 position dG electron-deficient carbon quinone, followed reductive cyclization loss (bromo-)1-hydroperoxy-benzene...
Abstract Background Vedolizumab (VDZ), a gut-selective monoclonal antibody for α4β7 integrin, approved in China treating inflammatory bowel disease (IBD), including ulcerative colitis (UC) and Crohn’s (CD). Here, we assess the improvements symptomatic relief quality of life (QoL) by patient-reported outcomes (PROs) from second interim analysis VALUE study (NCT04872491). Methods is prospective, multicenter, single-arm observational study. Enrolled patients with UC/CD aged >18 years,...
Under the auspices of Protein Analysis Working Group (PAWG) Comité Consultatif pour la Quantité de Matière (CCQM) a key comparison, CCQM-K115, was coordinated by Bureau International des Poids et Mesures (BIPM) and Chinese National Institute Metrology (NIM). Eight Institutes or Designated BIPM participated. Participants were required to assign mass fraction human C-peptide (hCP) present as main component in comparison sample for CCQM-K115. The samples prepared from synthetic hCP purchased...
ABSTRACT Introduction Pogostone possesses potent anti‐bacterial and anti‐fungal activities has been used for the quality control of essential oil Pogostemon cablin . is easily absorbed after oral administration but its metabolism in mammals remains elusive. Objective To investigate metabolic profile pogostone vitro vivo Methods High‐performance liquid chromatography coupled with mass spectrometry (LC–MS) techniques were employed. Orbitrap MS ion trap tandem (MS/MS) utilised to analyse by...
Cooperativity is essential for the proper functioning of numerous proteins by allosteric interactions. Hemoglobin from Scapharca inaequivalvis (HbI) a homodimeric protein that can serve as minimal unit studying cooperativity. We investigated structural changes in HbI after carbon monoxide dissociation using time-resolved resonance Raman spectroscopy and observed rearrangements Fe–proximal histidine bond, position heme pocket, hydrogen bonds between interfacial water upon ligand dissociation....
Under the auspices of Protein Analysis Working Group (PAWG) Comité Consultatif pour la Quantité de Matière (CCQM) a pilot study, CCQM-P55.2, was coordinated by Bureau International des Poids et Mesures (BIPM) and Chinese National Institute Metrology (NIM). Four Institutes or Designated BIPM participated. Participants were required to assign mass fraction human C-peptide (hCP) present as main component in comparison sample for CCQM-P55.2. The samples prepared from synthetic hCP purchased...
Hemoglobins achieve cooperative oxygen binding by diverse strategies based on different assemblies of globin subunits. Heterotetrameric hemoglobin from Scapharca inaequivalvis (HbII) consists two AB-dimers, whose structure closely resembles that homodimeric the same organism (HbI). Herein, we investigated structural dynamics HbII following carbon monoxide (CO) dissociation using time-resolved resonance Raman (RR) spectroscopy. The observed spectra showed heme transient dissociated form was...
Objective To identify the disease causative mutation in a Chinese family with congenital aniridia and to characterize its clinical phenotypes. Methods This was case series study. Eight participants were recruited from index family, including 3 patients, 2 asymptomatic siblings, spouses. All underwent detailed ophthalmic examinations. Peripheral blood samples taken all members for DNA extraction. coding exon-intronic boundary regions of PAX6 gene amplified sequenced this...
Abstract Background Conventional treatments for inflammatory bowel disease (IBD) have multiple potential side effects. Therefore, alternative are desperately needed. Exosomes from mesenchymal stromal cells (MSC-Exos) show potent immunomodulatory activities and protective effects in several diseases. Methods MSC-Exos were isolated human bone marrow-derived cells. Experimental colitis was induced by administration of dextran sulphate sodium (DSS) C57BL/6 mice (male, 6–8 weeks), or 2, 4,...
Time-resolved anti-Stokes Raman spectroscopy is selective for vibrationally excited modes, and it therefore a powerful method measuring vibrational energy relaxation. 1-2The intensity ratio of Stokes scattering expected to provide the most direct information about relative populations.Here, we applied this technique explore relaxation heme in globin matrix upon ligand photodissociation Scapharca inaequivalvis dimeric hemoglobin (HbI).Anti-Stokes time-resolved resonance spectra carbon...