A5023261593- Bacterial biofilms and quorum sensing
- Enzyme Production and Characterization
- Antibiotic Resistance in Bacteria
- Bee Products Chemical Analysis
- Protein Hydrolysis and Bioactive Peptides
- Probiotics and Fermented Foods
- Healthcare and Venom Research
- Porphyrin Metabolism and Disorders
- Microbial Metabolites in Food Biotechnology
- biodegradable polymer synthesis and properties
- Pharmaceutical and Antibiotic Environmental Impacts
- Evolution and Genetic Dynamics
- Microbial Metabolism and Applications
- Venomous Animal Envenomation and Studies
- Gut microbiota and health
- Antimicrobial Peptides and Activities
- Phytochemistry Medicinal Plant Applications
- Antimicrobial agents and applications
- Biofuel production and bioconversion
- Insect and Pesticide Research
- Essential Oils and Antimicrobial Activity
- Enzyme Catalysis and Immobilization
Cairo University
2018-2025
Agricultural Research Center
2023
Animal Health Research Institute
2023
University of Copenhagen
2018-2020
Georgia State University
2016
ABSTRACT The opportunistic Gram-negative pathogen Pseudomonas aeruginosa , known for its intrinsic and acquired antibiotic resistance, has a notorious ability to form biofilms, which often facilitate chronic infections. evolutionary paths resistance have mainly been investigated in planktonic cultures are less studied biofilms. We experimentally evolved P. PAO1 colony biofilms stationary-phase seven passages the presence of subinhibitory levels (0.1 mg/liter) ciprofloxacin (CIP) performed...
Abstract Background Polyhydroxybutyrate (PHB) has emerged as a promising eco-friendly alternative to traditional petrochemical-based plastics. In the present study, we isolated and characterized new strain of Salinicola salarius, halophilic bacterium, from New Suez Canal in Egypt exclusively potential PHB producer. Further genome analysis strain, ES021, was conducted identify elucidate genes involved production. Results Different PHB-producing marine bacteria were producers. Among 17...
Abstract Ciprofloxacin (CIP) is used to treat Pseudomonas aeruginosa biofilm infections. We showed that the pathways of CIP-resistance development during exposure biofilms and planktonic P. populations subinhibitory levels CIP depend on mode growth. In present study, we analyzed CIP-resistant isolates obtained from previous evolution experiments, report a variety evolved phenotypic genotypic changes occurred in parallel with CIP-resistance. Cross-resistance beta-lactam antibiotics was...
Lactic acid bacteria (LAB) are of major concern due to their health benefits. Fermented food products comprise variable LAB demonstrating probiotic properties. Discovering and evaluating new probiotics in fermented poses a global economic importance. Therefore, the present work aimed investigate evaluate potentials strains isolated from Egyptian food. In this study, we functionally characterized 100 bacterial different sources as probiotics. Only four amongst showed attributes considered be...
During chronic biofilm infections, Pseudomonas aeruginosa bacteria are exposed to increased oxidative stress as a result of the inflammatory response. As reactive oxygen species (ROS) mutagenic, evolution resistance ciprofloxacin (CIP) in biofilms under conditions was investigated.
Background Vitamin B 12 is a very important water-soluble vitamin, which was first isolated from the liver as an anti-pernicious anemia factor. The sole source of vitamin animal-based food. It has complicated structure and requires expensive multi-steps to be synthesized chemically. Intriguingly, can produced through microbial fermentation by microorganisms in cheap more effective manner. Objective This study aims isolate characterize that have capability produce . In addition, current work...
ABSTRACT Escapin is an l -amino acid oxidase that acts on lysine to produce hydrogen peroxide (H 2 O ), ammonia, and equilibrium mixtures of several organic acids collectively called escapin intermediate products (EIP). Previous work showed the combination synthetic EIP H functions synergistically as antimicrobial toward diverse planktonic bacteria. We initiated present study investigate how affected bacterial biofilms, using Pseudomonas aeruginosa a model. Specifically, we examined...
Vitamin B
The evolution of antimicrobial resistance (AMR) in biofilms, driven by mechanisms like oxidative stress, is a major challenge. This study investigates whether antioxidants (AOs) such as N-acetyl-cysteine (NAC) and Edaravone (ED) can reduce AMR Pseudomonas aeruginosa biofilms exposed to sub-inhibitory concentrations ciprofloxacin (CIP). In vitro experimental studies were conducted using flow cells glass beads biofilm models. Results showed that combining CIP with (CIP-AOs) effectively reduced...
Abstract Lactic acid bacteria are of major concern due to their health benefits. Fermented food products comprise variable lactic bacterial isolates demonstrating probiotic properties. In this study, we isolated and functionally characterized strains from different Egyptian fermented sources as probiotics. Only four showed attributes considered be safe for implementation feed or dietary supplements. Additionally, they were shown exert antimicrobial activities against pathogenic anticancer...
American foulbrood disease is one of the most deleterious diseases affecting honeybee colonies leading to a substantial economic loss in apiculture industry. The causative agent Gram-positive, spore-forming bacterium, Paenibacillus larvae. Honeybee larvae are more susceptible spores this particularly at onset their life cycle. Conversely, workers resistant P. spores. Many studies have focused on studying impact infection Apis mellifera L. However, few investigating effect pathogen adults...
Abstract Background PHB is of significant concern due to its potential applications as green alternatives traditional petrochemical-based plastics. In the present study, a new strain Salinicola salarius , halophilic bacterium, was isolated from New Suez Canal in Egypt and characterized exclusively producer. Further analysis ES021 genome conducted identify elucidate genes involved production. Results Different PHB-producing marine bacteria were producers. Among 17 bacterial isolates, showed...