A5047421381- Protein Kinase Regulation and GTPase Signaling
- Protein Structure and Dynamics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Bio-sensing Technologies
- Advanced Biosensing Techniques and Applications
- 3D Printing in Biomedical Research
- Enzyme Structure and Function
- Protein purification and stability
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Biotin and Related Studies
- Click Chemistry and Applications
SciKon Innovation (United States)
2021
Duke University Hospital
1997-1999
Duke Medical Center
1997-1999
Duke University
1997
Abstract The contribution to the free energy of binding each residues forming site for a human IgG Fc fragment on surface B1 domain protein G was determined by alanine‐scanning mutagenesis. interface between these two proteins is atypical in that it smaller than usual, polar character, and involves well‐defined “knobs‐into‐holes” interactions. bulk contributed three central residues, which make hydrogen bonds across interface. Of these, most critical interaction formed Glu27, acts as charged...
Single, extrinsic, environmentally sensitive fluorophores can be used to quantitate formation of protein-protein complexes. These prepared semi-synthetically by covalent coupling single cysteine mutations introduced at positions where the fluorophore is predicted respond complex without adversely affecting interaction. The three-dimensional structure a interface select such locations identifying residues that are located edge buried interfacial region, and in partial steric contact with both...
To test the relevance of calmodulin-peptide crystal structures to their respective calmodulin-enzyme interactions, amino acid side chains in calmodulin were altered at positions that interact with calmodulin-binding peptide smooth muscle myosin light chain kinase but not IIα peptide. Since shortening Trp-800, Arg-812, and Leu-813 abrogated calmodulin-dependent activation (Bagchi, I. C., Huang, Q., Means, A. R. (1992) J. Biol. Chem. 267, 3024-3029), substitutions introduced which contact...
Introduction: Because of the importance to create in vitro screening tools that better mimic vivo models, for exposure responses drugs or toxicants, reproducible and adaptable culture platforms must evolve as approaches replicate functions are native human organ systems. The Stairstep Waterfall (SsWaterfall) Fluidic Culture System is a unidirectional, multiwell, gravity-driven, cell system with micro-channels connecting 12 wells each row (8-row replicates). Materials Methods: construct...