A5016976575- Estrogen and related hormone effects
- Endometrial and Cervical Cancer Treatments
- 14-3-3 protein interactions
- Computational Drug Discovery Methods
- Ovarian cancer diagnosis and treatment
- Salivary Gland Disorders and Functions
- Proteoglycans and glycosaminoglycans research
- Protein Degradation and Inhibitors
- Laser Applications in Dentistry and Medicine
- RNA Research and Splicing
- Oral microbiology and periodontitis research
- Mesenchymal stem cell research
- S100 Proteins and Annexins
- Cancer Genomics and Diagnostics
University of Utah
2019-2023
Huntsman Cancer Institute
2022-2023
Temperature-responsive polymer grafted tissue culture dishes release cells as confluent living sheets in response to small changes temperature, with recovered cell retaining cell-cell communications, functional extracellular matrices and tissue-like behaviors. These features promote regeneration improve transplantation efficacy various tissues including cartilage, heart, kidney, liver, endometrium, cornea, middle ear, periodontium, esophageal sheet transplants. However, the effects of for...
New strategies for tissue engineering have great potential restoring and revitalizing impaired tissues organs, including the use of smart hydrogels that can be modified to enhance organization functionality salivary glands. For instance, monomers laminin-111 peptides chemically conjugated fibrin hydrogel (L1pM-FH) promote cell cluster formation in vitro gland regeneration vivo when compared with (FH) alone; however, L1pM-FH produce only weak expression acinar differentiation markers (e.g.,...
Activating estrogen receptor alpha (ER; also known as ESR1) mutations are present in primary endometrial and metastatic breast cancers, promoting estrogen-independent activation of the receptor. Functional characterizations cancer have established unique molecular phenotypic consequences receptor, yet impact ER has not been fully explored. In this study, we used CRISPR-Cas9 to model clinically prevalent ER-Y537S mutation compared results with ER-D538G discover allele-specific differences...
Our previous studies indicated that YIGSR-A99 peptides chemically conjugated to fibrin hydrogel (FH) and applied wounded submandibular gland (SMG) in vivo, formed new organized salivary tissue, whereas SMG treated with FH alone or the absence of a scaffold showed disorganized collagen formation poor tissue healing. While these damaged grow differentiate when containing L1 peptide, they were performed only female mice. However, there is well-established sexual dimorphism present mouse (e.g.,...
<p>ER mutants do not affect proliferation, but down-regulate proteins associated with movement and impact migration</p>
<p>Supplementary Materials and Methods</p>
<p>Differentially expressed genes from in vitro RNA-seq experiments</p>
<p>Identification of novel therapeutic targets in ER active and mutant endometrial cancer</p>
<p>Comparison of mutant ER regulated genes and by sustained estrogen signaling</p>
<p>Mutant ESR1 generation: gBlock used for template amplification</p>
<p>In vivo exploration of novel therapeutic targets in ER active and mutant endometrial cancer</p>
<p>ER-Y537S and ER-D538G mutants drive proliferative gene expression signatures in vivo</p>
<p>ER binding is altered by the Y537S and D538G mutations</p>
<p>Validation and gene expression analysis of ER mutant models</p>
<p>Enrichment values for ER associating proteins in endometrial and breast cancer cell lines</p>
<p>Differentially expressed genes from in vivo RNA-seq experiments</p>
<p>Overlap between prolonged E2 treatment and differentially expressed genes in vitro</p>
<p>In vivo exploration of novel therapeutic targets in ER active and mutant endometrial cancer</p>
<p>Supplementary Materials and Methods</p>
<p>Enrichment values for ER associating proteins in endometrial and breast cancer cell lines</p>
<p>Identification of novel therapeutic targets in ER active and mutant endometrial cancer</p>
<p>Mutant ESR1 generation: gBlock used for template amplification</p>
<p>Overlap between prolonged E2 treatment and differentially expressed genes in vitro</p>
<p>Differentially expressed genes from in vivo RNA-seq experiments</p>